FastQCFastQC Report
Tue 10 May 2022
EGAF00003664603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63826
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATG33895.3097483784037856No Hit
ATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCCG17712.7747313007238428No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATG16952.6556575690157618No Hit
ACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCCGTC10931.7124682731175382No Hit
ATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGCCG10701.6764328016795664No Hit
ACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGCCGTC7311.145301287876414No Hit
CATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCCGTCTTC3520.5514993889637452Illumina PCR Primer Index 8 (95% over 22bp)
CACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCCGTCT2950.4621940901826842No Hit
CACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGCCGTCT2110.3305862814526995No Hit
TTATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGC2000.3133519255475825No Hit
CATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGCCGTCTTC1840.28828377150377593No Hit
TATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCC1510.2365807037884248No Hit
TTATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGC1170.18331087644533575No Hit
ACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCTCGTATGCCGTCTT1030.16137624165700498Illumina PCR Primer Index 8 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGGGTGCCGTTTAATCGCGTATGCC940.14727540500736377No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGGTGCC830.13004104910224673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)