FastQCFastQC Report
Tue 10 May 2022
EGAF00003664615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55247
Sequences flagged as poor quality0
Sequence length100
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATG881115.948377287454523No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATG51269.278331855123355No Hit
ATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCG50259.09551649863341No Hit
ATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCG31505.701667058844824No Hit
ACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTC30665.549622603942296No Hit
ACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTC23594.269915108512679No Hit
CATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTCTTC19913.60381559179684Illumina PCR Primer Index 9 (95% over 22bp)
CATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTCTTC10051.819103299726682RNA PCR Primer, Index 9 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTCT7651.3846905714337432No Hit
CACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTCT6511.178344525494597No Hit
TCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTCTTCTG6381.1548138360453961Illumina PCR Primer Index 9 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGC5591.011819646315637No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCCCACGA4910.8887360399659711No Hit
TCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTCTTCTG4870.8814958278277554RNA PCR Primer, Index 17 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTCTT4700.8507249262403389Illumina PCR Primer Index 9 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCC4180.7566021684435353No Hit
TTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGC3640.6588593045776242No Hit
ACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTCTT2900.5249153800206346No Hit
TATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCC2750.4977645845023259No Hit
CTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGCCGTCTTCTGC1630.29503864463228774Illumina PCR Primer Index 9 (96% over 25bp)
CTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTATGCCGTCTTCTGC1040.1882455155936069TruSeq Adapter, Index 9 (95% over 24bp)
GCTTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTAT1010.18281535648994515No Hit
CGGAGATGTGTATAAGAGACACATCTCCGAGCCCACGAGACCCCACGACC950.17195503828262168No Hit
GCTTATACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCGCGTAT740.1339439245569895No Hit
CGGAGATGTGTATAAGAGACACACATCTCCGAGCCCACGAGACCCCACGA700.12670371241877387No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCCCACGA580.10498307600412692No Hit
GGAGATGTGTATAAGAGACACACATCTCCGAGCCCACGAGACCCCACGAC580.10498307600412692No Hit
CTTTACACATCTCCGAGCCCACGAGACCCCACGACCCAGATCTCGTATGC560.10136296993501909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)