FastQCFastQC Report
Wed 8 May 2024
EGAF00003664617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110923
Total Bases11 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATG27312.4620682816007498No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATG18411.659709888841809No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCCG16771.5118595782660043No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15761.4208054235821246No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATGCCG11591.0448689631546209No Hit
ACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCCGTC10270.9258674936667778Illumina PCR Primer Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.8925110211588219No Hit
ACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATGCCGTC8020.7230240797670456No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA7460.6725386078631123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.6391821353551563No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6080.5481279806712764No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA5090.45887687855539433No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA4530.4083914066514609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.3669211975875157No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3850.34708761933954185No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3530.3182387782515799No Hit
CATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCCGTCTTC3180.28668535831162156Illumina PCR Primer Index 1 (96% over 25bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2980.2686548326316454No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2970.26775330634764655No Hit
CACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCCGTCT2790.25152583323566796Illumina PCR Primer Index 1 (95% over 23bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2720.2452151492476763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2710.2443136229636775No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2580.232593781271693No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.2253815709997025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2490.2244800447157037No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2450.22087393957970844No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2410.2172678344437132No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2310.20825257160372512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2310.20825257160372512No Hit
CACATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATGCCGTCT2280.20554799275172866No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2250.20284341389973223No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.1983357824797382No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2070.18661594078775368No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2060.18571441450375487No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1970.17760067794776557No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1940.17489609909576911No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1940.17489609909576911No Hit
CATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATGCCGTCTTC1870.16858541510777747RNA PCR Primer, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1800.1622747311197858No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1790.161373204835787No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1760.15866862598379056No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1700.1532594682797977No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGC1680.15145641571180007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1650.14875183685980364No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1650.14875183685980364No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1640.14785031057580483Clontech SMART CDS Primer II A (100% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1640.14785031057580483No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1630.14694878429180602No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1610.1451457317238084No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG1600.1442442054398096No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA1600.1442442054398096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1580.14244115287181197No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1510.1361304688838203Clontech SMART CDS Primer II A (100% over 26bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1500.1352289425998215No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1500.1352289425998215No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA1460.13162283746382625No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1460.13162283746382625No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.13072131117982744No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1410.1271152060438322No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1380.1244106271918358No Hit
ACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCCGTCTT1350.12170604833983935Illumina PCR Primer Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCTCGTATGCC1330.11990299577184173No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCCCACCACGATCGCGTATGC1320.11900146948784292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1320.11900146948784292No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1300.11719841691984531No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC1280.11539536435184769No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA1280.11539536435184769No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA1250.11269078549985125No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA1230.11088773293185362No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1230.11088773293185362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1220.10998620664785481No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1200.10818315407985721No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.10818315407985721No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1190.1072816277958584No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT1170.10547857522786076No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC1160.10457704894386195No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1140.10277399637586433No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1120.10097094380786673No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1120.10097094380786673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1120.10097094380786673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)