FastQCFastQC Report
Tue 10 May 2022
EGAF00003664621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198486
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATG41622.0968733311165524No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCGCGTATG29131.4676098062331853No Hit
ATACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGCCG27611.3910300978406536No Hit
ATACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCGCGTATGCCG23561.1869854800842377No Hit
ACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGCCGTC17840.8988039458702376No Hit
ACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCGCGTATGCCGTC17140.863536974899993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.7350644377941014No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10450.5264854951986538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.4957528490674405No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9310.4690507139042552No Hit
CATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGCCGTCTTC6210.3128684138931713RNA PCR Primer, Index 28 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA5960.30027306711808394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.281128140019951No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5270.2655099100188426No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC5170.26047177130880766No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4870.24535735517870277No Hit
CACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGCCGTCT4730.23830396098465384No Hit
CACATCTCCGAGCCCACGAGACCCCGACCCAAACATCGCGTATGCCGTCT4380.22067047549953145No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4120.20757131485344055No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA4050.20404461775641608No Hit
CATCTCCGAGCCCACGAGACCCCGACCCAAACATCGCGTATGCCGTCTTC3940.1985026651753776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3680.18540350452928672No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG3650.1838920629162762No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3500.17633485485122377No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3460.17431959936720978No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3430.1728081577541993No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA3370.1697852745281783No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.1692814606571748No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA3330.16777001904416433No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA3170.15970899710810837No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA3050.15366323065606644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3020.15215178904305593No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2700.13602974517094404No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA2600.13099160646090907No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA2580.12998397871890208No Hit
TTATACACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGC2570.1294801648478986No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2510.1264572816218776No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2460.12393821226686012No Hit
ACATCTCCGAGCCCACGAGACCCCGACCCAAACATCTCGTATGCCGTCTT2430.12242677065384963No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2410.12141914291184264No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA2380.11990770129883216No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT2360.11890007355682516No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC2310.11638100420180769No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2300.1158771903308042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2290.1153733764598007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2240.11285430710478322No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2230.1123504932337797Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2190.11033523774976572No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA2180.10983142387876223Illumina PCR Primer Index 1 (96% over 28bp)
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT2120.10680854065274126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2090.10529709903973077No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2040.10277802968471329No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA2030.1022742158137098No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT2020.1017704019427063No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2020.1017704019427063No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA2000.10076277420069929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)