FastQCFastQC Report
Tue 10 May 2022
EGAF00003664651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46736
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATG11002.3536460116398494No Hit
ATACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCG8661.8529613146182813No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCGCGTATG6531.3972098596371105No Hit
ACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCGTC5691.217476891475522No Hit
ATACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCGCGTATGCCG4330.9264806573091406No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4290.9179219445395412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.8922458062307429No Hit
ACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCGCGTATGCCGTC3280.7018144471071551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.49426566244436837No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1840.39370078740157477Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1750.37444368366997605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1730.3701643272851763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1730.3701643272851763No Hit
CATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCGTCTTC1670.3573262581307771RNA PCR Primer, Index 2 (95% over 22bp)
CACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCGTCT1660.35518657993837727No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1570.3359294762067785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.33378979801437864No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1420.30383430332078054No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1160.2482026703183841Clontech SMART CDS Primer II A (100% over 26bp)
CACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCGCGTATGCCGTCT1140.2439233139335844No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1110.2375042793563848No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1080.2310852447791852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1060.2268058883943855Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1040.22252653200958575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1030.2203868538171859No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1020.21824717562478604Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC990.21182814104758643No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.20540910647038688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG920.19685039370078738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG910.19471071550838753No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA890.1904313591235878No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA870.18615200273878807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA860.18401232454638822No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT830.17759328996918863No Hit
CATCTCCGAGCCCACGAGACCCACTGAAAGGTATCGCGTATGCCGTCTTC830.17759328996918863No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA820.17545361177678878No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA810.1733139335843889No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC770.16475522081478947No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA740.15833618623758988No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA740.15833618623758988No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.15405682985279015No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG720.15405682985279015Clontech Universal Primer Mix Long (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGC660.14121876069839095No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC650.1390790825059911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT610.13052036973639164No Hit
ACATCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCGTCTT610.13052036973639164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG600.1283806915439918No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA590.12624101335159194No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA590.12624101335159194No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA580.12410133515919206No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG580.12410133515919206Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA530.11340294419719275No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT530.11340294419719275No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT520.11126326600479287No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.11126326600479287No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG520.11126326600479287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG510.10912358781239302No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT510.10912358781239302Clontech SMART CDS Primer II A (100% over 26bp)
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA510.10912358781239302No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA500.10698390961999314No Hit
TCTCCGAGCCCACGAGACCCACTGAAAGGTATCTCGTATGCCGTCTTCTG500.10698390961999314Illumina PCR Primer Index 2 (100% over 22bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG500.10698390961999314No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA480.10270455323519344No Hit
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT480.10270455323519344No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG480.10270455323519344No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC470.10056487504279356No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC470.10056487504279356No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)