FastQCFastQC Report
Tue 10 May 2022
EGAF00003664671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69740
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCTCGTATG7871.1284772010324062No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCGCGTATG7081.0151993117292801No Hit
ATACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCTCGTATGCCG6020.8632061944364784No Hit
ATACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCGCGTATGCCG5850.8388299397763119No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4640.6653283624892458No Hit
ACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCGCGTATGCCGTC4580.65672497849154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.5592199598508747No Hit
ACACATCTCCGAGCCCACGAGACCCTCATAGCACTATCTCGTATGCCGTC3660.5248064238600516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.47031832520791517No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2390.3427014625752796No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2320.3326641812446229No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1920.27530828792658446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.25379982793232003No Hit
CATCTCCGAGCCCACGAGACCCTCATAGCACTATCTCGTATGCCGTCTTC1730.2480642386005162Illumina PCR Primer Index 1 (95% over 22bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1650.23659305993690852No Hit
CATCTCCGAGCCCACGAGACCCTCATAGCACTATCGCGTATGCCGTCTTC1540.22082018927444794No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1380.19787783194723257No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1380.19787783194723257No Hit
CACATCTCCGAGCCCACGAGACCCTCATAGCACTATCGCGTATGCCGTCT1350.1935761399483797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1300.1864066532836249No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1210.17350157728706625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1120.16059650129050762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1100.15772870662460567No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1050.1505592199598509No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1050.1505592199598509No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1010.14482363062804704No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1000.14338973329509608No Hit
CACATCTCCGAGCCCACGAGACCCTCATAGCACTATCTCGTATGCCGTCT980.14052193862919415No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.13191855463148838No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA920.13191855463148838No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA910.13048465729853742No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA910.13048465729853742No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT900.12905075996558646Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.12331517063378261No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT790.1132778893031259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT760.10897619730427302No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA740.10610840263837108No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC740.10610840263837108No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT730.10467450530542013Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA730.10467450530542013No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT730.10467450530542013No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA720.10324060797246917No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT710.10180671063951821No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA700.10037281330656726No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)