FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5813
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT290.49888181661792536No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC260.44727335282986413No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC250.430070531567177No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA180.30965078272836744No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG140.2408394976776191No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT130.22363667641493207No Hit
GGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGG120.206433855152245No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG120.206433855152245No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT110.1892310338895579No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA110.1892310338895579No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA110.1892310338895579No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA100.1720282126268708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.1720282126268708No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT100.1720282126268708No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC100.1720282126268708No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT100.1720282126268708No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA90.15482539136418372No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG90.15482539136418372No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC90.15482539136418372No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG90.15482539136418372No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA90.15482539136418372No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG90.15482539136418372No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG90.15482539136418372No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG80.13762257010149664No Hit
ATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCA80.13762257010149664No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG80.13762257010149664No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC80.13762257010149664No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG80.13762257010149664No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA80.13762257010149664No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT80.13762257010149664No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT80.13762257010149664No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT80.13762257010149664No Hit
GAGTACATGGGAGCGTCATGCTAACTGATACACGCTGCTCGAACAGTGAT80.13762257010149664No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT80.13762257010149664No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA80.13762257010149664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA80.13762257010149664No Hit
GGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGA70.12041974883880956No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA70.12041974883880956No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC70.12041974883880956No Hit
GTGTTAAAGTGCTTAACCTCCATGGCAAGGGTGGTGGTGTCTCCATTTTA70.12041974883880956No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC70.12041974883880956No Hit
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT70.12041974883880956No Hit
GTACATGGGAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACC70.12041974883880956No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC70.12041974883880956No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC70.12041974883880956No Hit
ACTACAACCCTCGTAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTT70.12041974883880956No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT70.12041974883880956No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC70.12041974883880956No Hit
GTACATGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG70.12041974883880956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.12041974883880956No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA60.1032169275761225No Hit
GATGTGTACTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG60.1032169275761225No Hit
GGGTAACGAAGCGAACGTGGCGAACTGAAACATCTAAGTAGCTACAGGAA60.1032169275761225No Hit
GTCTAAGCCTAATTTGGAATGCTTTCAGTCTTACCCAGTCCAATAAAATT60.1032169275761225No Hit
CTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATT60.1032169275761225No Hit
GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT60.1032169275761225No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60.1032169275761225No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT60.1032169275761225No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA60.1032169275761225No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA60.1032169275761225No Hit
GCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACCAACATCGTTA60.1032169275761225No Hit
GTTTTATTGAGCAGAGTGACAGTACAAAGCTTACACAGCATGGAAGGGGT60.1032169275761225No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG60.1032169275761225No Hit
GTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCT60.1032169275761225No Hit
ATATTCCACCCTTTGAGTTACAATCGGGACAACTGGGAGAGGGGGAAGAG60.1032169275761225No Hit
GTATCAACGCAGAGTACATGGGAGTAGGATTTATTCAGGATCTTCCGACG60.1032169275761225No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC60.1032169275761225No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT60.1032169275761225No Hit
GGCTGTGAGTGTGGTTCCCATGCATGGACAGCTTAGGAACCTGGACAGCT60.1032169275761225No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG60.1032169275761225No Hit
CTTCATAGCTGTCAGAACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT60.1032169275761225No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG60.1032169275761225No Hit
GATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCA60.1032169275761225No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT60.1032169275761225No Hit
GGCTCGTACAAAGCAGACTGCCCGCAAATCGACCGGTGGTAAAGCACCCA60.1032169275761225No Hit
GGCAGAGAGAGGCCTCTTCCATGTCCTGGAGTTGAATGAGTTCAAAGATT60.1032169275761225No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC60.1032169275761225No Hit
CACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGA60.1032169275761225No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG60.1032169275761225No Hit
GAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACC60.1032169275761225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)