FastQCFastQC Report
Tue 10 May 2022
EGAF00003664683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157578
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.6288948964957037No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8660.5495691022858521No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCTCGTATG6810.4321669268552717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.369975504194748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.25447714782520403No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCGCGTATG3880.24622726522737945No Hit
ATACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCTCGTATGCCG3630.23036210638540913No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3460.21957379837286933No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3390.21513155389711763No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3350.21259312848240236No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3110.19736257599411083No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2540.16119001383441853No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2530.1605554074807397No Hit
ACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCTCGTATGCCGTC2230.1415172168703753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2220.14088261051669648No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2040.12945969615047787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1990.12628666438208377No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1880.11930599449161686No Hit
ATACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCGCGTATGCCG1860.11803678178425922No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1830.11613296272322278No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1830.11613296272322278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1770.1123253246011499No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1700.10788308012539823No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1680.10661386741804059No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1670.10597926106436178No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1650.10471004835700415No Hit
ACACATCTCCGAGCCCACGAGACGGGTATTGGTCCATCGCGTATGCCGTC1640.10407544200332534No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.10344083564964653No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1580.10026780388125245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)