FastQCFastQC Report
Tue 10 May 2022
EGAF00003664721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102999
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCTCGTATG9220.8951543218866203No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6620.6427246866474432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.4844707230167283No Hit
ATACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCTCGTATGCCG4720.4582568762803522No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCGCGTATG4200.40777094923251683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.3776735696463072No Hit
ACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCTCGTATGCCGTC3460.33592559151059714No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3250.31553704404897137No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2940.28543966446276176No Hit
ATACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCGCGTATGCCG2400.23301197099000961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.23204108777755122No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1800.17475897824250722No Hit
ACACATCTCCGAGCCCACGAGACCGAACAAGGACGATCGCGTATGCCGTC1710.16602102933038185No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1570.15242866435596464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1560.15145778114350625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1260.12233128476975505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1260.12233128476975505No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1230.11941863513237994Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.11941863513237994No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1200.11650598549500481Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1180.11456421907008806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1170.1135933358576297No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1120.10873891979533781No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1060.10291362052058758No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.10291362052058758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)