FastQCFastQC Report
Tue 10 May 2022
EGAF00003664816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56341
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27754.925365186986387No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8831.5672423279671999Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4660.8271063701389753Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3740.6638149837596067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3570.6336415754068973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3530.6265419499121422Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG3230.5732947587014785No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA2320.4117782786957988No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2270.40290374682735486Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2220.3940292149589109No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2180.38692958946415573Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1830.32480786638504816No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1770.31415842814291545No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1760.3123835217692267No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1720.3052838962744715No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1700.30173408352709397Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1560.2768853942954509No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1520.26978576880069577No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1390.24671198594274155Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1380.24493707956905272No Hit
GATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAG1360.24138726682167516No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA1350.23961236044798637No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1340.2378374540742976No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT1320.23428764132691998No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC1310.23251273495323121No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1250.2218632967110985No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1230.21831348396372088No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC1170.20766404572158817No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1160.2058891393478994Clontech Universal Primer Mix Long (100% over 23bp)
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG1150.2041142329742106No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.2041142329742106No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA1130.20056442022683305No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1120.19878951385314422No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC1090.19346479473207787No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC1080.1916898883583891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1916898883583891No Hit
CGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACAT1050.18636516923732274No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1040.18459026286363395No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA1030.18281535648994515No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1020.18104045011625639No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT1020.18104045011625639No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC1010.17926554374256756No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG1010.17926554374256756No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA970.17216591824781244No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG950.16861610550043485Clontech Universal Primer Mix Long (96% over 26bp)
GTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTGGTGAG950.16861610550043485No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG940.16684119912674605No Hit
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC940.16684119912674605No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG930.1650662927530573No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT920.1632913863793685No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT900.15974157363199093No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA900.15974157363199093No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA890.1579666672583021No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC870.15441685451092454Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC870.15441685451092454No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC870.15441685451092454No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA860.15264194813723575No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT850.15086704176354698No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT850.15086704176354698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG840.1490921353898582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG830.1473172290161694Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC820.14554232264248063Clontech Universal Primer Mix Long (96% over 25bp)
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG800.14199250989510304No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG800.14199250989510304No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG790.14021760352141424No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC780.13844269714772545No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC780.13844269714772545No Hit
GAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGC770.13666779077403668No Hit
GTGTTAATACCCGCACGCTTTAAGCAGTTGTAAGAACGAACAGAAGATCC760.13489288440034788No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA760.13489288440034788No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT750.1331179780266591No Hit
GTCATGGAGTGGGGTTCCCAGTTCAATCAGGAGCAAGGTGGCTGGGTTAA750.1331179780266591No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC750.1331179780266591No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC750.1331179780266591No Hit
CAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTC740.1313430716529703No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT740.1313430716529703No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG740.1313430716529703Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC730.12956816527928153No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG720.12779325890559273Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC720.12779325890559273No Hit
CTTCTAGTCTCGCTTTCACTTCTTCAAGTGATTTGCGTCCTAGATTTCGA720.12779325890559273No Hit
CGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTT710.12601835253190394No Hit
CCACCCGGCATTCGAGTCAACCCGGAGGGCGGCATCCGGCAGAACCGCGC710.12601835253190394No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT700.12424344615821516No Hit
GCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCT700.12424344615821516No Hit
CTCAGCAACTGGTCGCCATGGCTCACGGCGGCAGCGCCGGGGCTGATCTA700.12424344615821516No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT690.12246853978452636No Hit
GAATGAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATC690.12246853978452636No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA690.12246853978452636Clontech Universal Primer Mix Long (96% over 26bp)
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA680.12069363341083758No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCTGCGTGGC680.12069363341083758No Hit
GTACTATACATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT680.12069363341083758No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA670.1189187270371488No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG670.1189187270371488No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC670.1189187270371488No Hit
CCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCA670.1189187270371488No Hit
CTCTTGAACCGTGTTAATACCCACAACTGCTTTAAGCAGTTGTAAGAACG670.1189187270371488No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA670.1189187270371488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.11714382066345999No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG660.11714382066345999No Hit
ATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAAT660.11714382066345999No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT660.11714382066345999No Hit
GGTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGAC650.11536891428977122No Hit
GTCTCGGACTGCGTGGATCCACTCGACGTCGGCATCTACTTGGTAGCTTC650.11536891428977122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.11536891428977122No Hit
CAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTG640.11359400791608242No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA630.11181910154239363No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT630.11181910154239363No Hit
GAGTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC630.11181910154239363No Hit
AAAGAGTATTAATCCCCGTTTCTGGCGGTATACTGCGACACAATTCCACC630.11181910154239363No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG620.11004419516870485No Hit
GGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCA620.11004419516870485No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG610.10826928879501606No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC610.10826928879501606Clontech SMART CDS Primer II A (96% over 26bp)
CCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGA610.10826928879501606No Hit
CTTCAAGCATGAATTGTAGCTGACGGCAGGCCCCTAAACCCAAAAGTCGG610.10826928879501606No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT600.10649438242132728No Hit
TGCCTGGGCTTTGCAGATGCCTCCCCGGGAAACTCAGGGACCCCTCTATT590.1047194760476385No Hit
TCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGT590.1047194760476385No Hit
GAGTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG590.1047194760476385No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT590.1047194760476385Clontech Universal Primer Mix Long (96% over 26bp)
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA590.1047194760476385No Hit
AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG590.1047194760476385No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG590.1047194760476385No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT580.1029445696739497No Hit
CACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAG580.1029445696739497No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA580.1029445696739497No Hit
GAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTT570.10116966330026092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)