FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA947.349491790461298Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT735.7075840500390935Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG423.2838154808444098Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG352.7365129007036746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC231.7982799061767005Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT211.6419077404222049Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG211.6419077404222049Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC191.4855355746677092Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC141.09460516028147Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT141.09460516028147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120.9382329945269743Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCTCGTATGCCG120.9382329945269743No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT110.8600469116497262Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.8600469116497262Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA100.7818608287724784Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG90.7036747458952306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG90.7036747458952306Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA90.7036747458952306Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC80.6254886630179828Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70.547302580140735Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC70.547302580140735Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70.547302580140735Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60.46911649726348714Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA60.46911649726348714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC50.3909304143862392Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50.3909304143862392Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCTCGTATGCCGTC50.3909304143862392No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCGCGTATGCCG50.3909304143862392No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC40.3127443315089914No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC40.3127443315089914No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.3127443315089914Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGTGACCTTATCGACGCGCATGTGAGCCTATAGACAGCGATGTGAGC40.3127443315089914No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC40.3127443315089914No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAGCCCCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTAT30.23455824863174357No Hit
ATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTG30.23455824863174357No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT30.23455824863174357Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.23455824863174357Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCC30.23455824863174357Clontech Universal Primer Mix Long (96% over 26bp)
GGGCGGTATCAGCCTGTTATCCCCAGAGTACCTTTTATCCGTTGAGCGAT30.23455824863174357No Hit
TCAATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGC30.23455824863174357No Hit
GGTATCAACGCAGAGTACATGGGAATACAGGACTCTTTCGAGCCCCTGTA30.23455824863174357No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT30.23455824863174357Clontech Universal Primer Mix Long (96% over 25bp)
GTATATTCTTGCGTTAACGGTTTCCGCGTGCGATCTCTCTTGTGTTCAGA30.23455824863174357No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA30.23455824863174357No Hit
CACTAGAGCGACACCAACATCGTTACGCTTACACACCGGACGCTTGGATC30.23455824863174357No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.23455824863174357Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTGAGATGGAGTCTCGCTGTCGCCCAGGCTGGAGTGCAGTGGGGCGA30.23455824863174357No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG20.1563721657544957No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT20.1563721657544957No Hit
TCACTGAGCATCAGCGAGGATAGACTGATCCGCAGATGAGAGTGCTCACA20.1563721657544957No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA20.1563721657544957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT20.1563721657544957No Hit
TGTTGTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGTGCCACACGACTAC20.1563721657544957No Hit
GGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACC20.1563721657544957No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG20.1563721657544957No Hit
GCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTG20.1563721657544957No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG20.1563721657544957No Hit
GATATGAGCCAGTGCTGCTGCTCTCTACGGAGTGTGCTCGGCTATAACAG20.1563721657544957No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT20.1563721657544957No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA20.1563721657544957No Hit
TATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCG20.1563721657544957No Hit
ATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGC20.1563721657544957No Hit
CATAGTCACCACATAGATCGCGACATATCAGCATACATGGCATACTGACA20.1563721657544957No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.1563721657544957Clontech SMART CDS Primer II A (100% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1563721657544957Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CCACTGGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
ATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAG20.1563721657544957No Hit
ATTGAAGGCGTTGTGGAAGATGTTACAACGATTATCTTACACATTAAAAA20.1563721657544957No Hit
GAATGGAATCAACTCGAATGCAGTCGAATGGAATGGAATGGAATTAACCC20.1563721657544957No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA20.1563721657544957No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.1563721657544957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA20.1563721657544957Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTAC20.1563721657544957Clontech Universal Primer Mix Long (96% over 27bp)
CTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCG20.1563721657544957No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG20.1563721657544957No Hit
CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG20.1563721657544957No Hit
GAAAAAATGGATGCCCAGAGGACTCCCAACCCTGGCTTGGGGTCAAGAAA20.1563721657544957No Hit
CGTTAATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
CATTAGTATTTAGCCCTCTCACTGTGGGCCAAGTGACAACTTGTTCCTTC20.1563721657544957No Hit
GAGTACATGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCA20.1563721657544957No Hit
CACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCGCGTATGCCGTCT20.1563721657544957No Hit
GTACTTGGGAAGCATGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.1563721657544957No Hit
GTACATGGGAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATAT20.1563721657544957No Hit
ATTAAGGTGTTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTTC20.1563721657544957No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.1563721657544957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA20.1563721657544957No Hit
GGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATG20.1563721657544957No Hit
CTCCAGAACGGGGCGAACGTGAACCAAGCGGACAGTGCGGGCCGGGGCCC20.1563721657544957No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTGGCAAACAAGCTTTGATC20.1563721657544957No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCTCGTAT20.1563721657544957No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTTCATGTCCCGCAT20.1563721657544957No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
GAATGGAATGGAATCAACCACGACTGCAGGGCAATGGAATGGAATGGAAT20.1563721657544957No Hit
GGGGTGGGCAGGGTAAGGTGGCGTTGAAAGAAAAGGCATTTAGCAAGGTA20.1563721657544957No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG20.1563721657544957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG20.1563721657544957No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.1563721657544957Clontech SMART CDS Primer II A (96% over 26bp)
GAATTATTCTGAGAAACTCCTTTATTATGTGTGCATTCAACACACAGAGT20.1563721657544957No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT20.1563721657544957No Hit
CTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCC20.1563721657544957No Hit
AGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCTGGAGGAGAC20.1563721657544957No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1563721657544957Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
AATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATA20.1563721657544957No Hit
ACCACTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACTCGTGGGCTGCTATCTCGTATGCCGTCT20.1563721657544957TruSeq Adapter, Index 25 (95% over 21bp)
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC20.1563721657544957No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)