FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23118.060985144644253No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA886.88037529319781Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT665.160281469898358Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG312.4237685691946833Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG272.111024237685692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG251.9546520719311962Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC201.5637216575449568Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC191.4855355746677092Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT171.3291634089132134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC161.2509773260359656Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC141.09460516028147Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA110.8600469116497262Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA100.7818608287724784Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT90.7036747458952306Clontech Universal Primer Mix Long (100% over 23bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA90.7036747458952306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT80.6254886630179828Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG80.6254886630179828Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG70.547302580140735Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC70.547302580140735Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC70.547302580140735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60.46911649726348714Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC60.46911649726348714Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50.3909304143862392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.3909304143862392Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
CCTGTTACACTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAA40.3127443315089914No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC40.3127443315089914No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA40.3127443315089914Clontech Universal Primer Mix Long (96% over 26bp)
AGTATCACCATCGGGGGTAGAGCACTGTTATGGCTAGGGGGTCATCGCGA30.23455824863174357No Hit
GCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACT30.23455824863174357No Hit
ATATAAGCCGCTGTTGCGTAAATCAACGGCATGATCCCTATGACCGCGTC30.23455824863174357No Hit
ATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCG30.23455824863174357No Hit
GGTGTGAGACGAGCGAACTTGTTCGCGAAGCAATCGGAAGTGGTTCCAAG30.23455824863174357No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.23455824863174357Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.23455824863174357Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.23455824863174357Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.23455824863174357Clontech Universal Primer Mix Long (96% over 26bp)
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA30.23455824863174357No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.23455824863174357Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCC30.23455824863174357No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA30.23455824863174357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30.23455824863174357Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.23455824863174357Clontech SMART CDS Primer II A (96% over 26bp)
GGCGCGGTGGCTCACACCTGTAATCTCAGCACTTTGGGAGGCCGAGGCGG30.23455824863174357No Hit
CTCTTAAGCAGCGCACTTCTGCATAACTATGCCATACAGTTAGGTGCTAC30.23455824863174357No Hit
TTCCATTCCATTCCATTCCCCTGCACACGTGTTCATTCCATTCCATTCCT20.1563721657544957No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG20.1563721657544957No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC20.1563721657544957No Hit
TACTTGGATAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG20.1563721657544957No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA20.1563721657544957No Hit
GTACATGGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.1563721657544957Clontech Universal Primer Mix Long (100% over 27bp)
GTTGTGGCACCGTGATCCAGTATATGAGCTACCGAGTCGTTCTGATAGAT20.1563721657544957No Hit
GGGTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACC20.1563721657544957No Hit
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA20.1563721657544957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.1563721657544957Clontech Universal Primer Mix Long (96% over 25bp)
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG20.1563721657544957No Hit
GTAAAGGTTCATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATC20.1563721657544957No Hit
GGCTCAGCTTCTCCGGGTCGTCTGACGCCATGAGGGAGAAGTCGCGGAAG20.1563721657544957No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT20.1563721657544957No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.1563721657544957No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGCAAGTTAGTGAGATTCCATTATTGGGAGATGATTTGCTTCAATGA20.1563721657544957No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG20.1563721657544957No Hit
ATGTAACGCTATGGCGTGCTAGTCGTCTCATATACGTCTGATGACCTCGT20.1563721657544957No Hit
GTACATGGGGTCGGCTATGATTTGCAATGCTTGCAGTATGTATCCTGATC20.1563721657544957No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
CAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCG20.1563721657544957No Hit
ATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAA20.1563721657544957No Hit
CCTGTATATCGCGTTGCAGGGCTCTTAAGCTGGATTCAGTCGTGTCAAGC20.1563721657544957No Hit
CACTAAGCTTCAGCTACACACGACCGTACCGCAAACCGACACTGGTGCGC20.1563721657544957No Hit
CTCTATAGCAACAGGCCTGGAGGTGCTGCAGTAGTGGGGGAAAATGGAAG20.1563721657544957No Hit
GTACATGGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGT20.1563721657544957No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
ATTCCATGTGGGCCAACTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGC20.1563721657544957No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.1563721657544957Clontech Universal Primer Mix Long (96% over 25bp)
CACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG20.1563721657544957Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.1563721657544957Clontech SMART CDS Primer II A (96% over 26bp)
GATGGGTTCGACTCTGTGAGTTGAATGCACACCTCACAAAAAAGTTTCAC20.1563721657544957No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT20.1563721657544957No Hit
TCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGC20.1563721657544957No Hit
CTAATATCCTTCCTTATGTCCCGCATTAAACAGCACTCAGCATCTCAAAC20.1563721657544957No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA20.1563721657544957No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG20.1563721657544957No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT20.1563721657544957No Hit
GTCTCCTCCAGAGACTCAGCGAAGTTGAAATGTTTGTGATGATGCAATCT20.1563721657544957No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG20.1563721657544957No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.1563721657544957Clontech Universal Primer Mix Long (96% over 26bp)
GGGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACA20.1563721657544957No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)