FastQCFastQC Report
Tue 10 May 2022
EGAF00003665153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155496
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12570.8083809229819416No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC11180.7189895560014405No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC11090.713201625765293No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT6490.41737408036219587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.414801666923908No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5500.35370684776457273No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT4870.3131913361115399No Hit
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT4700.3022585789988167No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA4520.29068271852652156No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4380.28167927149251426No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4150.2668878942223594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.2347327262437619No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3520.22637238256932654No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3210.2064361784225961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT3090.19871893810773267No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC3080.19807583474816073No Hit
GTACATGGGCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3050.19614652466944488No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT3040.19550342130987292No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3040.19550342130987292No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2970.19100169779286927No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC2930.18842928435458148No Hit
GTACATGGGCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT2910.18714307763543755No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2900.18649997427586562No Hit
TATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTC2850.18328445747800587No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2790.17942583732057416No Hit
GTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGC2770.17813963060143026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.17042239028656686No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2640.1697792869269949No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA2490.16013273653341564No Hit
GAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA2340.15048618613983641No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG2340.15048618613983641No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2240.14405515254411688No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG2170.13955342902711323No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC2150.13826722230796934No Hit
ACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC2120.13633791222925348No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAACGGCTAAATCTCGTATG2040.13119308535267787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2040.13119308535267787No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT2020.12990687863353398No Hit
GAGTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCG1930.12411894839738642No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA1850.11897412152081083No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC1840.11833101816123887No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1840.11833101816123887No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG1790.11511550136337911No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1730.11125688120594741No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1710.10997067448680353No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG1700.10932757112723157No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1680.10804136440808766No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC1670.10739826104851573No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1670.10739826104851573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT1670.10739826104851573No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG1630.10482584761022792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA1620.10418274425065596No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG1610.10353964089108401No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG1580.10161033081236817No Hit
TATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCG1560.10032412409322426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)