FastQCFastQC Report
Tue 10 May 2022
EGAF00003665257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465226
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCTCGTATG34800.74802354124662No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32630.7013795445654369Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCTCGTATGCCG20190.43398262349911654No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCGCGTATG20150.4331228263252699No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20030.4305434348037298Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.3645540017109964No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT16660.3581055229071462Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15360.3301621147571288No Hit
ATACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCGCGTATGCCG13120.28201347302171414No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12230.2628829859036253Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11930.25643450709977517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11680.25106077476323335Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.24912623112207827No Hit
ACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCTCGTATGCCGTC11460.24633189030707656No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10870.2336498819928379Clontech Universal Primer Mix Long (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAATCCCGAATAAATCGCGTATGCCGTC8190.1760434713451097No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA8120.174538826290878No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT7450.16013722362894595Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7440.15992227433548425Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7210.1549784405858658Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT7190.15454854199894244Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.15304389694471074No Hit
CATCTCCGAGCCCACGAGACAATCCCGAATAAATCTCGTATGCCGTCTTC7000.1504645054231707Illumina PCR Primer Index 11 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA6870.14767016460816895Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6450.1386422942827787Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA6130.13176391689200517Clontech Universal Primer Mix Long (95% over 21bp)
TCCTAAGACCTGGCACATGTTAATTTTAATGATTATCTGGGTGAATGTAT5800.12467059020777No Hit
TCCTTGGCCTGTGCCCAGGGCCCTGAGTTCTGGTGCCAAAGCCTGGAGCA5760.1238107930339233No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.11886695928430484Clontech Universal Primer Mix Long (95% over 22bp)
GTGTATGCCATGCCGTAGCCCTGCTCCTCTGTGGTGTCATCCACATCGAC5520.11865200999084315No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT5410.11628756776276476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA5350.11499787200199474No Hit
GTATATAATCAGCCAGTTGGAGCTGCAGGGGTACCATGGATGCCAGCGCC5340.11478292270853305No Hit
GCCCTAGGGCATTGCCTACAGGAAGTCTGGGGACATGTGGGAGCCGATGA5280.11349322694776302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5120.11005403825237625No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA5080.10919424107852958No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA4910.10554010308968115Illumina PCR Primer Index 1 (96% over 28bp)
CTATTATACAGAGTTATAATATAAAAATGATTTAAAGGCTATATTTTTCA4710.1012411172204477No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT4670.100381320046601Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)