FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTTCCACCTCGTGACCGAGCCCTAGCATGTTCAATAGTAACCCTTT23.7735849056603774No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT23.7735849056603774No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTT23.7735849056603774No Hit
ACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCA23.7735849056603774No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC23.7735849056603774No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA23.7735849056603774Clontech Universal Primer Mix Long (96% over 26bp)
ACTACAACCAGAGCGAGGCCGGGTCTCACACCCTCCAGTGGATGTATGGC23.7735849056603774No Hit
GAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGAC11.8867924528301887No Hit
CCTCATGCTAGCCTCACGAAACTGGAATAAGCCTTCGAAAAGAAATTGTC11.8867924528301887No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG11.8867924528301887No Hit
GGTGACGGGACTGGAAGCCCTTTTCAGAGCCTTGGAGGAGCTGGTCCGTC11.8867924528301887No Hit
GTTAATATACATTTATTCGCGCAAAATCATCTATCCCAGTCTTCGAGCCG11.8867924528301887No Hit
GAGGGGGGATCATTTTAGAGAGTGGGTCATTGGCAGGGAGTGCTCCGGAG11.8867924528301887No Hit
GTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGAT11.8867924528301887No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.8867924528301887No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.8867924528301887No Hit
GTGCAATGGCGTGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCAA11.8867924528301887No Hit
ATATAAGAGCGAGGCCCTCGGCGTGGGAGAAGTGGCCCTCCCGGGGCAGG11.8867924528301887No Hit
GTTTTGTTTACAAACTGTTAATGCATGTTCGAATTGTTGTAGGGGAGTTA11.8867924528301887No Hit
AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATATCCTTT11.8867924528301887No Hit
TGCCAGAAGCCAGGGGCTCAAAGGGGGCCATGGTGGGTTCTCTGGCTGTC11.8867924528301887No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTGTCTA11.8867924528301887No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTACCT11.8867924528301887Clontech Universal Primer Mix Long (95% over 22bp)
CAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGC11.8867924528301887No Hit
GTCAGTGGATACAAGTAGTGCAGAAGGTTCACACTGCAAATAGTGTTCTC11.8867924528301887No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC11.8867924528301887No Hit
ACACTGCTTATCTTGTGTTCTCCCCAAGCAAGAGCTTTGTCCACCTTGAG11.8867924528301887No Hit
GTAACATTTTGGCTGAGGTAATTATTAGCTTAATTCCCCAAATGAGCGGG11.8867924528301887No Hit
CCCTAGAGCCACCCCCGCCCCCCGTCAAACCGCCGCAGCCATGGGCAGGC11.8867924528301887No Hit
CTTTTTGACTGCAAAGTGCAAAAGATTCCTGAACAAACTGCCTGCACATT11.8867924528301887No Hit
ACTCCCACCGCGAATCCAAAACATACACGTCGTGTCAGCGAGTGGAGGTG11.8867924528301887No Hit
CCTCACAGTTGTTTACCAACCTGAAGATATTCTTGTCTACTCCATTGAAT11.8867924528301887No Hit
CAGATATCTCTTGCAACACAAATGGTTAGAATGATTTTGAAGATTGATGA11.8867924528301887No Hit
GTCTTATATTACAAAAATGGGGGTTAAGGAGAAGTGTTTATGTGGGTTTA11.8867924528301887No Hit
GGCCCGCCATCAGTTCCTGGAAGTAGTGCAGGTTGTGCCAGGTCAGAAGC11.8867924528301887No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAACGT11.8867924528301887Clontech Universal Primer Mix Long (95% over 22bp)
GTGAAGGGCGCATGTTCCCGGTGCAGATGCGTTGGGGTCGACCCTACCAG11.8867924528301887No Hit
CTGCTGCTCATCGCATTCGCGTTGGGTGCCTTCTTCGAGGGCGCAGCGGG11.8867924528301887No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT11.8867924528301887No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG11.8867924528301887No Hit
GAAGCGCTTTGGAGCGGACGCTGGCGGCCTGACCGCGCTGGTCAAGAGCG11.8867924528301887No Hit
GCGAGCAGGGCGGGGAGCAGCGCGTCGCGCAGCGTGACGCCGTGCAGCGC11.8867924528301887No Hit
GCCCAGGCTGGAGTGAGATCATGGCTCACTGCAGCCTCCACCTCCCAGGC11.8867924528301887No Hit
AGCGACACATAAATTGTCGAGTACGGAGGCGCAAGGTGCGAGCAAAGGAT11.8867924528301887No Hit
GATTGCACACGGTTTCTTCAGCGTCTATGGCATGTGTGTGGACACGCTGT11.8867924528301887No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)