FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGCGTGGAGGTCGACGACTCCGTCGCAGACTACGGACCTGTCTGGG23.7735849056603774No Hit
CTCCAGGTAGGCTCTCCACTGCTCCGCCTCACGGGCCGCCTCCCACTTGC23.7735849056603774No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA23.7735849056603774No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA23.7735849056603774No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23.7735849056603774Clontech Universal Primer Mix Long (96% over 26bp)
GATTGAGAGAGTGAGGAGAAGGCTTACGTTTAGTGAGGGAGAGATTTGGT23.7735849056603774No Hit
GGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTA23.7735849056603774No Hit
GAATATCTCTATCAGGAACAGTATTTTATTAAAAAGGATAGCAAAGAAGA11.8867924528301887No Hit
ACCTCCGGCAGCCCGAGCTTGGTCTTGTGATGATCCGTGGCGATCCGCGC11.8867924528301887No Hit
GAGTGCCAGACGTAGGAACCTATATCACCTCCACTCGCTGACACGACGTG11.8867924528301887No Hit
GGCACCAGGAGCGAGAAGAACGGCAGTTGCCGGCCGATCATTCCCGACAG11.8867924528301887No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG11.8867924528301887No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT11.8867924528301887No Hit
ACGCGGCGCGGTCCCGTCGCCATCAAGAACGCCCGCCATGCCGACGACCC11.8867924528301887No Hit
GTTCTGGCCAAAGACATCAGCTAAGAAAGGAAACTGGGTCCTACGGCTTG11.8867924528301887No Hit
GATGGGAGGTGGCTTCCCCAAGGAGTGAAGAGTTCATGGGTTGGCTCAGT11.8867924528301887No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGAGTACTTTTT11.8867924528301887No Hit
GAATCAGTGACAGAATCAGCTTGAGAAATTAAAATGCCGCTAAGAATGCC11.8867924528301887No Hit
GCCGCAGACTCTCGCAGTAATGGCGGCCGCACCAGGGTGCACCATCCTTC11.8867924528301887No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT11.8867924528301887No Hit
CTTTGGAGTTGCATGCTTGCAACGTGGAAATGTGTTTCCCACAGCCCCAC11.8867924528301887No Hit
GCCCCAGACTCTCCACGTCCAGAAACTGGTGGGATCTGGCTCATGGGGAG11.8867924528301887No Hit
GGTTCCATGAGGGGACTTTGTTCATGATCAGTAGCACTCGCTTCTTGTCT11.8867924528301887No Hit
GAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTG11.8867924528301887No Hit
GCTGGACTCAGGCGATCCCCGCGCCGCCTCGCTCACCGCCGCCCTGTGGC11.8867924528301887No Hit
CTTTTAATACAATGAATACAAAAGCAGTTGAGATAGTACCCCCACGATAA11.8867924528301887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTAACACAGCTTCCGCC11.8867924528301887No Hit
GGCTCAGGGTATGGAACCGGCCTTCTTTCAGCCAACAGACATTCACTCAT11.8867924528301887No Hit
CCAGAGAACCCACCATGGCCCCCTTTGAGCCCCTGGCTTCTGGCACTGTC11.8867924528301887No Hit
GAGTACATGGGCTCAAGGTGGACAAAGCTCTTGCTTGGGGAGAACACAAG11.8867924528301887No Hit
CTTAACCAATTGATTCTTCCTCGACAGGAATCCTCAGTCATTACACACAG11.8867924528301887No Hit
GCTGGCACAAGACCTTCTTACCGCAATTTACGGGTGCATCCGCAACGAAG11.8867924528301887No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA11.8867924528301887No Hit
GTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTG11.8867924528301887No Hit
ATCTGATACCAGAATTTCAATTGGTATTAGAGCAGGCATCCTGCTCTGGT11.8867924528301887No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC11.8867924528301887No Hit
GGAGATGCGCTGGGTGTCGAGGCGGGTCATGCCGCTGCCGGGGTCGAAAC11.8867924528301887No Hit
TTGCAGCGGCGGCCTTCATCCTGGCCCCGATCACGCCGGCCAGCGTTATC11.8867924528301887No Hit
CGGCATGTCTGAGGAAGAGAAGAAAGCCATTGTGGTGGGAGTGAGGGGCA11.8867924528301887No Hit
ACTACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCG11.8867924528301887No Hit
ACCCTCTCTCTACAAAAAATTAAGGGTTAGCCAGGTGTGGTGGCTTGTGC11.8867924528301887No Hit
GGGGCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAA11.8867924528301887No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTACCCCCAGTGGG11.8867924528301887No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA11.8867924528301887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATAACAATGCTTACCCT11.8867924528301887No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA11.8867924528301887No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)