FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTG33.061224489795918No Hit
GTATAGAAGCTGATAGAATATTTTGGTGGTGGCATTCTCAGCATACATTT22.0408163265306123No Hit
GATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG22.0408163265306123No Hit
AAATAAACTATGCTGTTTTATTTATCTTCCCAACCTGATTGGCAGCTAGA22.0408163265306123No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.0408163265306123No Hit
CTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACT22.0408163265306123No Hit
AGGTGGAGGGCCCCATTTCCCCAAAATCACAGCCTAGTGACGTAGGAGCA22.0408163265306123No Hit
CCTGAGGACCGAGCAGTGGCCAAGGAAGCTGTACATGCAGCTCATCCCGC22.0408163265306123No Hit
GAACAAAACACACATCGGCAGGGCATGGTGGCTCACGCCTGTAATCCCAG22.0408163265306123No Hit
TACCTGCAGGCAGCTTTTCCTAAGCCCAAATGGGACTGTCAATTATTTGA11.0204081632653061No Hit
GGGTGGTGCTGGCCAGCGAATCGGGCGTGCTCGACATCGACCCGGCCGAC11.0204081632653061No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG11.0204081632653061No Hit
TGACAAGACGTTTTAAATTAAAAACCTGTAGTATAACTGTCTCTTATACA11.0204081632653061No Hit
GCCCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG11.0204081632653061No Hit
GAATAGGCCCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.0204081632653061No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGGGTGGTG11.0204081632653061No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.0204081632653061No Hit
GTTTAGGTTTCTGTTCCGATCGGATTCAGCTGGCATGTTTTCCAATTCCG11.0204081632653061No Hit
CTTTACAATTTAGCATAATTTTGCAGTGGCTAGTACCGATTTTTCCTTTC11.0204081632653061No Hit
CACTAAAACACAGAAATCTTGTTTCTGTTCTTGTGTCAGATGAACCGGTT11.0204081632653061No Hit
GTGTACGACGCGCACACCAAGGAGATCGACCTGGTCAAACGCGACCCTAA11.0204081632653061No Hit
CTCTGAGACTTGCACCAGCAGTCCCCCAGGTCTCAGGCCGTCAGCCTCAA11.0204081632653061No Hit
ACTTAAGTTGGTCCTTTACACTCAGGCTTTCACTATTTCCTTTAAAATGA11.0204081632653061No Hit
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG11.0204081632653061No Hit
AGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG11.0204081632653061No Hit
CCTGGTCTCCGGGCCAGGGCAATGTTCCGCACGGCAGTGATGATGGCGGC11.0204081632653061No Hit
ACCCAGACTTCTGAATTCTCAAACAAAGATGAAAATATGTCCAGTTTGAG11.0204081632653061No Hit
TCCAGATCTTTTCTGTTCAGTGTACTTTGGGCAGCCATGCTGACGGCCTC11.0204081632653061No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11.0204081632653061No Hit
AGCAAAGATAGCAGAAAACTCTAGGTTTTTGCCAATTTATTTTTTCCTAG11.0204081632653061No Hit
TCACAATCCACTGCAAAAATAATGTTTGTGATAAGAAATTTGAAAGTTGA11.0204081632653061No Hit
TAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCC11.0204081632653061No Hit
GAGTACAGGGGCTGGGTCAGAGGTCAGGAATTACGGCACAGGTGAAACCT11.0204081632653061No Hit
ACAGTGGGCAGGCGGCGCGCCCAAGCTCCCCGACCACAAGCCCGGAAGGT11.0204081632653061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGGG11.0204081632653061No Hit
TGACATTCCGGCTTAGCTTTAAGGCGGGCGGCAGAGTGGGCAATTGATCC11.0204081632653061No Hit
GAGTAAGGCAGTGGTTCTCAGGCCCAATTGCACAATAGAATCACCCTGGG11.0204081632653061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.0204081632653061No Hit
CTCCACGTCCCAGCTCCACATGGCCACCGCGTTCCACTTCTTGAGGGAGA11.0204081632653061No Hit
CCCGAAGTGCTGGGATTACATGCGTGAGCCACTGCGCCCAGCCAACAGTG11.0204081632653061No Hit
CCATACATTTAGCCACTCTGGGTAGTAAGCAGTGTGGAAAGAGCACAGAA11.0204081632653061No Hit
GACTACAGCCTGGTCGAGGCGATCCTGGCGGGACGCGCCTGAGGACCTGC11.0204081632653061No Hit
GCTCACTGCGTCCTTGAGCTCCTAGGCTCAAGTGATCCTTCCACCTCGGC11.0204081632653061No Hit
GTGTACGACGCGCACACCAAGGAGATCGACCTGGTCAACCGCGACCCTAA11.0204081632653061No Hit
CTGTAAAACCTCCTGCAAATAGGAAAGGATTGTTTTCTGGCATTTTAGAT11.0204081632653061No Hit
TTTCTCGATCGTAACTTCGCGGGGGAGGTGGGTGATTACATTTCGGTCAT11.0204081632653061No Hit
ACATTCTATTTAAGCCCACCTTTAGTTCCCTAATTCCAGGATCCCCAGAA11.0204081632653061No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.0204081632653061Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTGAGCTGGGGTGGTGACATATTTCCATTCATCAGACTATGAGACAA11.0204081632653061No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC11.0204081632653061No Hit
CCGTACCTTCTTTCATCGACATCCCCAATTTCGGCATGACCACTTCGACA11.0204081632653061No Hit
CCCCCGACGACATGGCGGGCGTGTCCACAACCGCCGCCAGCTTTCCGTCC11.0204081632653061No Hit
TCCCAAGGTGGGTTAATTGGAAAGCCTGTTTACTTTTAAACTAAACTGCC11.0204081632653061No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11.0204081632653061No Hit
TCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCG11.0204081632653061No Hit
TAAGGATACTTATCTGATACAAATAGTGAGGGCCAGGCATAGTAGCTCAC11.0204081632653061No Hit
CCTCGATCCATCGCGTATAGGGACTCCCAATGCTCGCGTTACTGCCAAGC11.0204081632653061No Hit
CCCTCTTTCTTTGGAATCTGCAAGGGGATATGTGGACCTCGTTGAAGATT11.0204081632653061No Hit
AGTGCTTTGAGGCATATGGTAAAAAAGGAAATATATTCACATAAAAAATA11.0204081632653061No Hit
ATGCAGCTACTTGTAAAAATGAAACATGTGAAATCTACTTGTCTATCTAA11.0204081632653061No Hit
GTCCAGGAACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGA11.0204081632653061No Hit
ATCCACGGATTGCCATGGTGGTGGTGGTCGAAAACCCGGTGACCAAATAT11.0204081632653061No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11.0204081632653061No Hit
AGGCTGTATAAGAATTGGAGGTGGGCCATCCTGGCTAACACGGTGAAACC11.0204081632653061No Hit
CCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATA11.0204081632653061Clontech SMART CDS Primer II A (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAAGAG11.0204081632653061Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATT11.0204081632653061No Hit
TGCCAGGGTACATGGTGGTGCCGCCAGACAGCACTGTGTTGGCGTACAGG11.0204081632653061No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTCGCGCGCGTGCAGCCCCGGAC11.0204081632653061No Hit
ATTTAATAGATTGCCTTTTGTTTAGTTGATAGTTTTCTTCAATGTAAAAA11.0204081632653061No Hit
CCGCATGGCCGCGCTGGGCATCGCGCGCACCTTCCAGAACCTGGAGGGTG11.0204081632653061No Hit
GTATTCTAGAACATGCCCTTCACTGGTTGTGTAAATAAAACTGTAGAATG11.0204081632653061No Hit
AGCCAGACCCTGTTACAAAAAGAGAGAGAGAGAGAGGGAGAGAGGGAGGG11.0204081632653061No Hit
GCACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTG11.0204081632653061No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA11.0204081632653061No Hit
GTTGTGAGCCCTGGAGATGAGGAGGAACAGGCAAAGGAAACTTAGCAGCA11.0204081632653061No Hit
GTTGAATGCCACTGAAGGCATATTTTGCTAAACTGCTGCTCCAGGTATTT11.0204081632653061No Hit
CTCTCCATGGTATTTGCCTCTGGTGGTTCATTCAATGTCTTCACCAAAAA11.0204081632653061No Hit
GTAAAAGAAATTTTGCAGAAATTAAATTAAGAATCTTGAAATGGGGAAAT11.0204081632653061No Hit
CTGTTATCTCATGCGTCTAGCCTGAAGTGATGGCGCGGCTGACGGTGGAT11.0204081632653061No Hit
ACTCTCGTGGCAGCTCTTCCAGACGCTGAGTCTTCGGGAATTGCAGTTGG11.0204081632653061No Hit
GTGTGGGGGGCCACCTCTGTGGGACCCAGGCGGGCTGCGTCCGGGTCCAC11.0204081632653061No Hit
GGGTTGACCAACAGACCACCCTGGTCGGCGCCGATCAGGTCCTCGTAGAT11.0204081632653061No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG11.0204081632653061No Hit
GGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCACT11.0204081632653061No Hit
TATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCG11.0204081632653061No Hit
TTGCTGGTCCATGCGCGGACCTTGATGTCGTAAGTGGTGTCTGGCTTGAG11.0204081632653061No Hit
GGGTTGACCAACAGACCACCCTGGGCGGCGCCGATCAGGTCCTCGTTGAT11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)