FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTTTGTCCAAAATTTTCCCGCCTTGCTGATCGAAACCCATCTACCCC37.6923076923076925No Hit
CCATCGGCCCGCCCCCGACCAGCAGCAGCCGCGCGTACGGCCCTGTCTCT25.128205128205128No Hit
CCGTCGACAACGTCGGCCACGCGGTGCACTACTCCGCACCGGCCGTCACC25.128205128205128No Hit
GTAAATCCGAACGCTTCCGCTAGCAACGCCACTGCAGCACTGCGGGAGCT25.128205128205128No Hit
GTATCAAGAGTCGGAGCGGTCGAGCGGCGCGGCCGGGCCGCAGCGCAACG25.128205128205128No Hit
ATATGATTTCATATAATGAACACATTGATTTCCGGAGGACCCCATTGTAT12.564102564102564No Hit
CCACAGAGGTGGTCGCGCAAATTCGGACCAGGGGCTAAGCTCTGTCTCTT12.564102564102564No Hit
TGGTGAGGGTGACGCCGAGCACGCGGTGGGCGAGCTTGGCGTCGTCCCAG12.564102564102564No Hit
AGATTAAGCGCGGCGAGTGCTCCGGCGTCGGTCGTGACGTCGTTGACCAG12.564102564102564No Hit
CTGGGGCGCGGAGCGGAAGTTCTGGGACTGTCTCTTATACACATCTGACG12.564102564102564No Hit
GCGGTGAGTTCCAGCCTGGCGCCCAGGCGAACGGGATCTGCTGACGGTTA12.564102564102564No Hit
ACTCAGTGATTTCGTGAGCAGGTTGCAGGTGCTGCTCCTGTCTCTTATAC12.564102564102564No Hit
GTTCACAACTTCATCTACCGAGAGGTTATGTCCAAGGTCTGTCTCTTATA12.564102564102564No Hit
GGCCGATGATGGCCAGGTTCTGCGCGTACGGGGCTGTCTCTTATACACAT12.564102564102564No Hit
GGGCGATACGCTGACGCTGGACGGCGAGACCTTCACGCTGAAGCAGTTCC12.564102564102564No Hit
AGCCTCTTCGGCTTCTGCTTGAGCTGCCTCCAATACCATCTGTGGTGCTG12.564102564102564No Hit
GGCGTGAACAGCCTGCGCGAAGGCGAGCACGAGGCTGGGCAGGTGCGCGT12.564102564102564No Hit
CACCGGGGTGGAGTCCAGGTGCCCGCGCAGGTGGTCGAGCTCGACCTCGG12.564102564102564No Hit
GCTCATCGTGAGGCTCACGAACCGGGCGTCGCAGGTGAACAGGTTGCCCC12.564102564102564No Hit
GTGAAACCCCTAAAGACAGCGATACCGTGGTGGTTAACTACCTGTCTCTT12.564102564102564No Hit
CCACAGAGGTGGTCGCGCAAATTCGGACCAGTGGCTAAGCTCTGTCTATT12.564102564102564No Hit
TGCTCGCCCTGCTCGCTGGCCTGATGATGATGCTGACGGCCGTCGGTCTG12.564102564102564No Hit
CACGTGGGCTATTATCGACACTCGACCCGGCGCTGCTGGCTGGTCGCCCG12.564102564102564No Hit
TCAAAATCCTGAAAGTGCATGGAATTTTGATGCACCGATGTTTGAAAAGA12.564102564102564No Hit
GACGATGTGTTCCGGGCCGCGGAGGATCTGGGCATCGACCTGTCCCGCCG12.564102564102564No Hit
AAAGGGCTCCCAGGGGATCAAAGGAAAAATTCAGTAAAAGGGCCTCAAAA12.564102564102564No Hit
GATTTGCAGTAAACCGTGAATACGTCCGCCACGGTCAATCACCGGCAAAA12.564102564102564No Hit
GTTGTTCTCGCTACGGTCGATGCGAAGAGTCAGGTTCTTGCCGAGCTGGT12.564102564102564No Hit
GATGCAGTGCGACCTGATGAATGCCTACTTCAAGCTGTCCCGCGACCTGA12.564102564102564No Hit
TGTCGGAGGTTGCATAGCGAAAGACTCTTTGCATAGAGTGCTCTTCATGA12.564102564102564No Hit
CGACCATCCCTTAGCTGCGCGAACAGAAAGCCGCGCCCGTCGCCCTGTCT12.564102564102564No Hit
GGCGCACCGCGCTCGGAACGCTCGACACCACGCTGACCCGCAACGAGACC12.564102564102564No Hit
AGTCGTGCTCGCGCATAAGGGCGACGGCCTGAGCATGCTTGCCGGCGACC12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)