FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66.8181818181818175Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG55.681818181818182Clontech Universal Primer Mix Long (96% over 26bp)
CACCTGCTTGATCTAAGTTTTTCGCATTTGCGGCTTCAGATTTTACTGTC33.4090909090909087No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.4090909090909087Clontech Universal Primer Mix Long (96% over 26bp)
CAATACCGCCCTCACGGATATGCTTGGCGCGCCGTGTCATCTGTGGGGCG33.4090909090909087No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT33.4090909090909087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCAGCGGAGAGGGAAGATCAACGTGGATCGGCCCGGGGAGTGGACCGG22.272727272727273No Hit
ATCGCGAGCGCCTGCACCGGCTTGTTGCTCGAGCGCGGCAGCACCGGCAC22.272727272727273No Hit
GTCTTCATCAGCGCGGCCGAGGTTAAATCCTTCAAAACCAGCACCCCCGC22.272727272727273No Hit
GCAAGACGACGAAGCGCACCACTATGCAAGATACGTGCGCGGTCGTGGGA22.272727272727273No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
TTTGGGTCCCGCGCTAACCTTGTTGCCCTGAAACAACTCACCTCGGAACT22.272727272727273No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
ATGATCGGCACCCTCGTCGGCACGCGGTGGTTCATCCATTGGCTCGCCGC22.272727272727273No Hit
ATGTCCTCGATACCTTGTCCGAGCGAGAGGCCGGTGTCGTGTCGATGCGA22.272727272727273No Hit
TTCTAATAGTGAAGTATTTTCGAAACTATACAGTCAAACTTGAAGAGGAA11.1363636363636365No Hit
CTCCGTGCGGACCGCTGACTCCCTCTACCTTGGGTTCCCTCGGCCCCACC11.1363636363636365No Hit
GGGAGTGGCCTCAGCTCTCCCAGACCTCATAACACAAGTGTTACACTTTT11.1363636363636365No Hit
CTACGTCCCGGGGACCCATGCAGTCTGTGTCAGCTGTACGTGACGGGTCC11.1363636363636365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCGGCATGCGACCTTGCTCAAAGTACACCTTGAAACCGGTCGGACTC11.1363636363636365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT11.1363636363636365No Hit
ATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGG11.1363636363636365No Hit
GTCATGATGGGCACCAACTTCTGCTTGCTCTCCTCCATGGACACGAAGCT11.1363636363636365No Hit
CTACTACATCACCAAACTCGGCGGGGACAAAAAGGTCAACAAGCTCATCG11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA11.1363636363636365Clontech SMART CDS Primer II A (100% over 26bp)
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG11.1363636363636365No Hit
GACACAGACTGACAATCAAGAAGTCTACAGCAGGATGGGAAAGGCGGGCA11.1363636363636365No Hit
CACTAAAAGTGGGATATTTTGGGGTGGCATGCTCTGGTCCCCTTCTACAT11.1363636363636365No Hit
ATACCTGCTTATCCCGTATTTTCACTGACGTGAAGTGCGGAAGCTGCCGG11.1363636363636365No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
GCGCGTAGTCGAAACTGCGTCCGGCACGCTCCCGATCGCCCAACACCTTG11.1363636363636365No Hit
GCAACTCGTCGAGGCCGGGAATCGGCTTCATGAGACGAACGTGGCGGTCT11.1363636363636365No Hit
TTTTTCAACTGTCAAATGAGTTAGTGATAACTTCTCAGTTGAAAGCTCAC11.1363636363636365No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGCATCA11.1363636363636365No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG11.1363636363636365No Hit
ACGTGGGAATTATGGGAGCTACAAGATGGGATTTGGGTGGGAACACAGAG11.1363636363636365No Hit
AAGGGAAAGCTGAGCCGCTACTCGGTAGTACGCCACCACGTTGACAGCTA11.1363636363636365No Hit
CCTTTATAGATCGGGTTGTCCGCCCTGAGGACGCTCGCACCGCAGCGGCG11.1363636363636365No Hit
CTCCGTCACTGGGCGGCGGGGCAGCTCGAGAGCCTGGGCAAGCGCGTGCC11.1363636363636365No Hit
CATGAGCACAGGTCAGCCCAAGGACACTAATCATGGGTTTCTGGGCTTGT11.1363636363636365No Hit
GATCATCCCAGAAGTCTAGGAGTTGATTCTTCTCATTTCTTCAGTTCTGT11.1363636363636365No Hit
GTCCTCGGGAAGCTCCTCAGCACACCACGTCACGATTCGTCCCAGATTGG11.1363636363636365No Hit
CTACTTGCTGGAGCTATCAAGGTGTCCGGGCCACCACCACAGCTACCGGA11.1363636363636365No Hit
GATTGAAGAGATCATTCCGGAGCCGAAGGGCGGTGCCCATCGCAATTACG11.1363636363636365No Hit
TTCCAGGACCTTCTGGGCGTGCTCTGCATCTAGTTCAGCTGGCGCCCTCG11.1363636363636365No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
ATCACGTAGATCGTCAGGTCGGAGAGTTCGGGGCTGAAGGTCGCGGCCAA11.1363636363636365No Hit
GTGGAGACGCGCTCCTGATTGCCCACAATGTCGTCAGTGGTGATAGTGAA11.1363636363636365No Hit
CTGGTGGGGTCGTCGGGGCAGTGACGGTTCTAGTTGGAGCAGCTGGTCTC11.1363636363636365No Hit
ATGGATAGATGCGCAGCAAATCGGTATCGTATCGCGCACATGCCTGTTCC11.1363636363636365No Hit
CGGTCAAAGTGTGCTGGTCCGAGACTCCAGCCAGTACCGTCACGATGCGG11.1363636363636365No Hit
CCCTCACCATGATGTCGGCCTCAACCTTCCTTATTGGCATTCTGCCCGAC11.1363636363636365No Hit
CGTATCAGTAGGTGTTCAAAATGGGGCATAGTACCCAGCGAGATTTCGGA11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
TGGTATCAACGCAGAGTACCTGGGAAGCAGTGGTATCAACGCAGAGTACA11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
ATCATAGCTTACGCCATAAGTCCAGTTGATTTTTTCTTTTTGCAGGTACA11.1363636363636365No Hit
GTGTGAGGGAATCAGGAACATTTTTTGCTGTCCCAACCTATGCTTTCATG11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)