FastQCFastQC Report
Tue 10 May 2022
EGAF00003665755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055425
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC33690.31920790202998794No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT29360.2781817751142905No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG23400.22171163275457756No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT21800.20655186299358078No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT21590.20456214321244995No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT19130.18125399720491747No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG17590.1666627188099581No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT16170.15320842314707345No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG15740.14913423502380557No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT14890.14108060733827604No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT14540.137764407703058No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC13980.13245848828670914No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT13350.12648932894331666No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC13340.12639458038231044No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA13090.12402586635715471No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG12750.12080441528294289No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT12730.12061491816093044No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG12350.1170144728426937No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC12280.11635123291565008No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG12050.1141720160125068No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC11740.11123481062131368No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC11590.10981358220622024No Hit
CCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCT10770.10204420020370941No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG10670.10109671459364711No Hit
CTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTT10640.10081246891062842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)