FastQCFastQC Report
Tue 10 May 2022
EGAF00003665761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27346
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1040.38031156293424995No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT850.31083156585972355No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC780.2852336722006875No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG690.25232209463906974No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA670.24500841073648796No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG640.23403788488261537No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG630.2303810429313245No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG480.17552841366196154No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG480.17552841366196154No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC460.16821472975937982No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT450.16455788780808894No Hit
CTGTAAGGCAAGATCTTTGTCATCAGGGACGGGAAGAGACGGATAAGATA430.1572442039055072No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT420.15358736195421632No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT420.15358736195421632No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG420.15358736195421632No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC410.14993052000292548No Hit
ACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGT410.14993052000292548No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA400.1462736780516346No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT390.14261683610034376No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT370.135303152197762No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT360.13164631024647117No Hit
GTACATGGGGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGGCT360.13164631024647117No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG360.13164631024647117No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA360.13164631024647117No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC350.1279894682951803No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG350.1279894682951803No Hit
CTCCTGGACCCAGGACTCACTGGGGTCAGCGCAGACTTGCTTGCCTCTTT340.1243326263438894No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA340.1243326263438894No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA330.12067578439259855No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT330.12067578439259855No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA330.12067578439259855No Hit
GTATCAACGCAGAGTACATGGGAGGACGTGGACCATTACTGTCCCAACTG330.12067578439259855No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT330.12067578439259855No Hit
GACCATTACTGTCCCAACTGCAGAGCTCTCCTGGGACCTACAAGCGTTTG320.11701894244130769No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC320.11701894244130769No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG320.11701894244130769No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC320.11701894244130769No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA320.11701894244130769No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG310.11336210049001681No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT310.11336210049001681No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG310.11336210049001681No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA310.11336210049001681No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC310.11336210049001681No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA310.11336210049001681No Hit
CTTAAATCCTGATCTTCATATCGCGACTCTTGGTGAGAATGCGAGTTTCC310.11336210049001681No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT310.11336210049001681No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC310.11336210049001681No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT310.11336210049001681No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG310.11336210049001681No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA300.10970525853872595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA300.10970525853872595No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG300.10970525853872595No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTTAGCATC300.10970525853872595No Hit
GTACATGGGAGAATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTT300.10970525853872595No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT290.1060484165874351No Hit
GACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGAGATGCGGCTCATG290.1060484165874351No Hit
GGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG290.1060484165874351No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA290.1060484165874351No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT290.1060484165874351No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT290.1060484165874351No Hit
TTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGA280.10239157463614423No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG280.10239157463614423No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG280.10239157463614423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)