FastQCFastQC Report
Tue 10 May 2022
EGAF00003665762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27346
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT1270.46441892781393984No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT1080.39493893073941344No Hit
GTACATGGGAGGACGTGGACCATTACTGTCCCAACTGCAGAGCTCTCCTG850.31083156585972355No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC680.2486652526877788No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG660.2413515687851971No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG580.2120968331748702No Hit
GTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAG570.2084399912235793No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA520.19015578146712497No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG520.19015578146712497No Hit
AGATAATGGTAGGCACTAAAGGCTGGTTCAGCCGAGCTCTGGGCAGAACA520.19015578146712497No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT490.1791852556132524No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT490.1791852556132524No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA460.16821472975937982No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT460.16821472975937982No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG460.16821472975937982No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG430.1572442039055072No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT390.14261683610034376No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT380.13895999414905288No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC370.135303152197762No Hit
GGACAGGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT370.135303152197762No Hit
GTATCAACGCAGAGTACATGGGAGGACGTGGACCATTACTGTCCCAACTG370.135303152197762No Hit
GTATGGAGGAGATGCGGCTCATGGAGAAGCATCCAGGCTCAGGTGACCTT360.13164631024647117No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC360.13164631024647117No Hit
GGACACAGCTAGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG360.13164631024647117No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC360.13164631024647117No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA360.13164631024647117No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA350.1279894682951803No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT350.1279894682951803No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA340.1243326263438894No Hit
GTATCAACGCAGAGTACATGGGGACAGGACACAGCTAGGTTCTGAAGCTT340.1243326263438894No Hit
TCCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATA330.12067578439259855No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA330.12067578439259855No Hit
GCGGAGAGGAGTCCTGAGTATGGAGGAGATGCGGCTCATGGAGAAGCATC330.12067578439259855No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA320.11701894244130769No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC320.11701894244130769No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA320.11701894244130769No Hit
ATATTACACACGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATC320.11701894244130769No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTTAGCATC320.11701894244130769No Hit
GTACATGGGAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTG320.11701894244130769No Hit
GATGAAGAGAAGACGCTAGAAATTGATGTACAGGGTGAAGGAACTGTAAC320.11701894244130769No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG320.11701894244130769No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA310.11336210049001681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG310.11336210049001681No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG310.11336210049001681No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT310.11336210049001681Clontech Universal Primer Mix Long (96% over 26bp)
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT310.11336210049001681No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG300.10970525853872595No Hit
CATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGA300.10970525853872595No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT300.10970525853872595No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA300.10970525853872595No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG300.10970525853872595No Hit
GTACATGGGAGAATCAATGAGTGAATGAATGAACGAATGAATGTGTTTTT300.10970525853872595No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT290.1060484165874351No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG290.1060484165874351No Hit
GACAGGAACTGCGGAGAGGAGTCCTGAGTATGGAGGAGATGCGGCTCATG290.1060484165874351No Hit
CTATGAGACCAGCAGCCTCTGCTCCCAGCCAGCTGTGGTATTCCAAACCA290.1060484165874351No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT290.1060484165874351No Hit
ATCTTACACATTAAAAAGCTTGCATTGAAAATCTACTCTGATGAAGAGAA290.1060484165874351No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA290.1060484165874351No Hit
TTGATGTACAGGGTGAAGGAACTGTAACGGCAGCTGATATTACACACGAT280.10239157463614423No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG280.10239157463614423No Hit
ACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACTGCGGAGAGGAGT280.10239157463614423No Hit
CGATAGTGATGTAGAGATCTTAAATCCTGATCTTCATATCGCGACTCTTG280.10239157463614423No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG280.10239157463614423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)