FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG62.510460251046025Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.6736401673640167Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41.6736401673640167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCATAACGGACGTGTATGGTTCCCCGGCTTGCGAAACAGCACGCCGACG31.2552301255230125No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.2552301255230125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT31.2552301255230125Clontech Universal Primer Mix Long (96% over 26bp)
ACCTATGTAGAGAAAATTGTACGGCCCATTACCCGAACGCGTATTCGGGA20.8368200836820083No Hit
CCCTATTCAACCTCTCCTCCCAGCCCATTGTCTTCCTGTTTTGCTACCTC20.8368200836820083No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGACGGCGACA20.8368200836820083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTTCCCT20.8368200836820083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGTTGAGGTGGTCCGGCACGCCGCAATAGACCGCGGGCCGCAGCGCGCG20.8368200836820083No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.8368200836820083Clontech Universal Primer Mix Long (100% over 23bp)
CCGTCACCGTCCGGACAGGCCCCGCTCGTCGTCGGCCTCGATCTTGGCGG20.8368200836820083No Hit
TATCGACGCCCATGCCGGTGCCGATGTCGGCCGACGTGGCCCTGCATTTC20.8368200836820083No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA20.8368200836820083Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.8368200836820083Clontech Universal Primer Mix Long (96% over 26bp)
CCGATCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.8368200836820083Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCGCACCAGCATCCACAGGCGGTCAAGCCGGTGGGCGCTGTCGCGCG20.8368200836820083No Hit
AGGCCAAAGCCTCTGCACAGAGTGTCCTCGCTGACCCTCCAGCCCAGAGC20.8368200836820083No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCT20.8368200836820083Illumina Single End PCR Primer 1 (100% over 33bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT20.8368200836820083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGCGTGTGACTTTGCGCAACCCTTCAAACCGGTGCGCTCTTCGATCTCA20.8368200836820083No Hit
GTGTCTGTGAATTAAAAAGGAAAAGTAGAAATAAGTAAAACTCAGGTTGA20.8368200836820083No Hit
GATTAGAAGACCCTGTCTTTCACAGACCCTGCCTGCCTGGGTCTGTACTC20.8368200836820083No Hit
GTGTTGAACACAACTGACAAAATCAGCAGCCTTACTGAGCTTCTATTCAA20.8368200836820083No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.8368200836820083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGGTGCCCCATTGTTATGAAGTGTGGAAACTGAGACGCTGGACTAGTCT20.8368200836820083No Hit
GGTTCTAAGACCCCGTAGTCTCAAACGTAAAGGGTTGCTGCCAAGGTGAT20.8368200836820083No Hit
TGTTGAATGTGCATTTCTGCAAACAACAGATTTTGTACTGGATTAATAAG20.8368200836820083No Hit
GTCAGTGCCAGGGGGCTCCCCGCCGGCCATGGCCGCGTCGAGGGCATCCC20.8368200836820083No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGATGTACGGCG20.8368200836820083Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.8368200836820083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACGTCGTCGGGGCCACCGCGGGCCGGATCGATCCCGCATTGCTGTACGA20.8368200836820083No Hit
GCTGGGACTACAGACATGTGCCACCATCCCTGGATAATTTTCATATTTTT10.41841004184100417No Hit
CTTACATTTTGATAAGGGTGAAGACAGTAGGAATGAGCCCCTATTATTTG10.41841004184100417No Hit
GCCAGTAATGCCCTGTAGGCTCTATCCCTACTATCACGTTCTCTTTGCCA10.41841004184100417No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GCCATGTACTTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATA10.41841004184100417Clontech Universal Primer Mix Long (95% over 22bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.41841004184100417Illumina Single End PCR Primer 1 (100% over 21bp)
TAAGGAAGGGGACCCGGGAGGTCCAACAGTTGCCGGCGTCGCCAGATGCA10.41841004184100417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTAT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGGCAAAAAAAAA10.41841004184100417No Hit
GCTTTTTGACTTCTAGACCTTAGACACTAAAAATAATGAAATAGATTAAT10.41841004184100417No Hit
TCCTCGCGGTGCAGGTCGTCGCGGGCGTGATCACCGCGGTGGTGCTGGGG10.41841004184100417No Hit
GTGGCATCAACGCAGAGTACATGGGATGTACCCACACCGAGATACACTCT10.41841004184100417No Hit
AATATAGATAATGGCGGCTGATGAGCCCGAGGTCGTCCTCGGTGACGACT10.41841004184100417No Hit
TCGTTGCCGGTGTCGACCGAGATCACGGTGTCCACCGACAGGAGGTACCG10.41841004184100417No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10.41841004184100417Clontech SMART CDS Primer II A (100% over 26bp)
TTACAGCTCATTGCAGCCTCAAACTCCAGGGCTCAAGCAATCCTCCCACC10.41841004184100417No Hit
GTTTCAGCATCATTTGCAGAACAGGTTTTTCCTTTCTCCATTGGCATCTA10.41841004184100417No Hit
GTACATGGGGTCGGGGCCACCAAAACTCATTACCAAAACCACGCGGGGTT10.41841004184100417No Hit
GACTACCCCTTCACCACGCTGCACCCGAACCTGGGTGTCGTGCGCGTCGG10.41841004184100417No Hit
CACCAGCGGGTCGCGCAGCTCGTTCGGGCCGGGCGCGCGCTCGACCTCCA10.41841004184100417No Hit
AGATAAGGTGAAGAACAAGTAATTTGGGTTGACAATTCTCAAATTGTCAA10.41841004184100417No Hit
CCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCCCCTGCCGGC10.41841004184100417No Hit
GAGGAGCTGGGGAAACAAATAAGCCAATAAACTCGCTCCAGGCAAAAATG10.41841004184100417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACAGCTA10.41841004184100417No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACGACTGCTTCC10.41841004184100417Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
ACGGGCGCGTGCGGCGGCTTGTCCGCCGAAACGGGAAAGGCCGCCCGAAG10.41841004184100417No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACCAAGGGTAC10.41841004184100417No Hit
CCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGG10.41841004184100417Illumina Single End PCR Primer 1 (100% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGCCCCATTT10.41841004184100417No Hit
ATCCGCCATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAG10.41841004184100417No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATGATACGGCGCCACCGAGATCTACACTCTTTCCCTACACG10.41841004184100417Illumina Single End PCR Primer 1 (100% over 30bp)
CGGTTCTCGTTGAGCGGGGCCAACAGTTGGATCATGCCGTCGGTTTCCTT10.41841004184100417No Hit
TAATTAATAAAAGGGCAATTCAGCCCAAGATTGCTTTATATAGCATTATT10.41841004184100417No Hit
ATTTTGAATTCCCTGTCTGAAAGGTATTCCCTATCTGATAGATCTCTATC10.41841004184100417No Hit
GTCAGATGTGTATAAGAGAAAGCCGTATCATTCCCATGTACTCTGCGTTG10.41841004184100417No Hit
CCCCTCGAGCCTGCTGATGTCGCTGTCGCAGACCCCGAACGTGGTGGTGC10.41841004184100417No Hit
GGGTGGAGCTGTTCGCCACCCGGATCCGCTTCAGCGGGTTCTCCGTCGGT10.41841004184100417No Hit
GTTGTGTGCTTCTTTATTTTAGTCTCTACTAGGCTCTGGCAAGGAAAACA10.41841004184100417No Hit
CCCGGCGTCGGACCAGCAGCCCGACATCGCGGTGATCCCGGGCACCACCT10.41841004184100417No Hit
TACCAAGGCTGGTCTTGAACCCCTGGGCTCAAGCCATCCTCCCGCCTCCG10.41841004184100417No Hit
TATCTGGAGGGAGAGCATCCAGATGGAAGGGACAGACCAGACAAAGGCCT10.41841004184100417No Hit
GCTTCAAATTCAGCAATTTGTAGATTCAGATCATGTGACTAGGACAGCAC10.41841004184100417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10.41841004184100417No Hit
GAAGAGCGTCGTGTAGGAAAGGTGTAGATCGTGGTCATTCCCATGTACTC10.41841004184100417No Hit
GTCTTCAGTCCTGAAACCGCAGTAGTTCGGAGAACTGAGGCGCTCGGCGC10.41841004184100417No Hit
TGTTCGAGGCGTTCCCGCGCGTGCTGGACAACTACCGCCGCCGGTTCCGC10.41841004184100417No Hit
CTCTTGGCAGGGGCCGCCCCATCGTGTCCGCTAGTGCCTCCCTCACCATC10.41841004184100417No Hit
GGACACAACATGGCAGTTGGCCCACATGGAATATCGGTTTGAGGCCCCGA10.41841004184100417No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGGTTTTAA10.41841004184100417No Hit
GTGCGATGGCGTGCAGGGGGCGCAACCTGTGGTGACGCGCGATGCGCCGG10.41841004184100417No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
TGTTACATCTGTGTGAAGTCCTATTTATCTTTTTTCTTTTCTGCATATAC10.41841004184100417No Hit
AAAGAACTCTCACAACTCAACAAAAATCAAAATAACCTAATTAAAAAATG10.41841004184100417No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.41841004184100417Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTCTTCCCATGTACCTGTCTCTTATA10.41841004184100417Clontech SMART CDS Primer II A (95% over 22bp)
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGC10.41841004184100417No Hit
GTACATGGGGTAGCGACACCATCCACTCTTTCCCTACACGACGCTCTTGC10.41841004184100417Illumina Single End PCR Primer 1 (96% over 27bp)
TCCCAAGACTGAACTAGGAAGAAGCTGAATCCAGGAATAGACCAATACAG10.41841004184100417No Hit
GAATCCGGACCTCATCTCCTCGGCTATCGAGGAACAGCTTCGATTCGTCG10.41841004184100417No Hit
GCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAA10.41841004184100417No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGGTATCAACGCAGAGTACAT10.41841004184100417No Hit
GCATGTGGATATCCAGTTGTCCCAGCACCATTTGTTGAAAACATTATTTT10.41841004184100417No Hit
GGTGCAAGTAGGCGACCAGCAGCAGGACTGCTGTCACCCAGAGGGTGAGG10.41841004184100417No Hit
CTCGTGTGAGGTACAACCGCTCCGTGTGGGCGGAGGCGCCGGCCAAGCTG10.41841004184100417No Hit
GTATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGT10.41841004184100417Clontech Universal Primer Mix Long (100% over 23bp)
GGTCAACCGCGCGGACCTGCGGCAGCTGGACGCCGTGGGCGCCGCCGTGC10.41841004184100417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGATCGCCTGCGGCTTGAACCCGAACGGGCCGAACGCCTCGTTGAAT10.41841004184100417No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTAT10.41841004184100417Clontech SMART CDS Primer II A (96% over 25bp)
CCACCGCTCTTCCGATCTCACTGTCTACTGGTACCAGCAGGGAGATCGGA10.41841004184100417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTATTTA10.41841004184100417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTGCGTTTATACCACAGCTTCCCAAGT10.41841004184100417No Hit
GGGTACTTATATCGGCTTGGCCGCCGGCGATGAAATGCGCGGGCACTTCG10.41841004184100417No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG10.41841004184100417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGGGTTTTAACCAATGATAC10.41841004184100417No Hit
ACACTGTACCAGACTATGAGATGAGAATCTCTTATACTCTGTCTCTTATA10.41841004184100417No Hit
CTCTTCCGAGCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGG10.41841004184100417No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT10.41841004184100417No Hit
GGTGCGCCGCTGACGGCTCCCCAGACGCGGTCCGGGGCCGGACTCCGGGC10.41841004184100417No Hit
TACGAACCGAGATCTACACCTTCCCTACACGACGCTCTTCCGATCTCTGT10.41841004184100417Illumina Single End PCR Primer 1 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.41841004184100417Clontech Universal Primer Mix Long (95% over 21bp)
CTATATCCCCATATATATTCTTATGAGGTATTTATTTCTCACTTTAAGTG10.41841004184100417No Hit
CGCCGGCAGACGCCGCGGCATGGCGCGCCGTCGCAGCGCCGTTCGTCACA10.41841004184100417No Hit
CCCTCATTCATCCCAGATTAAAAACAAACAAAAAAAACCTACAAAACCTG10.41841004184100417No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGGGGGTTTTAACA10.41841004184100417No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACCTGT10.41841004184100417Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAATAAAACCCTTCTAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.41841004184100417No Hit
TCTGTACATAAAAGTTAAAAATTAACCAGACAGTCTGGGCGCAGTGGCTC10.41841004184100417No Hit
GAAGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAGTACCTGTCTCTTAT10.41841004184100417No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAT10.41841004184100417No Hit
ACATGGGACCGCACCGAGTCTACACTCTTTCCCTACACGACGCTTGCCTC10.41841004184100417Illumina Single End PCR Primer 1 (96% over 26bp)
GTAAAATACATAGCTACATATCCCTGTGAGATAAAAATACCTTTCCACCT10.41841004184100417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.41841004184100417No Hit
ATGCTGGATCATGTGGTAATTCTATTTTTCCTTCTTCTAAGTTTTCTTAC10.41841004184100417No Hit
GTTGGTACCAGATCGGAAGAGCGTCGTGGAGGGAAAGGTGAGACTGGGCG10.41841004184100417Illumina Single End PCR Primer 1 (96% over 28bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAATGCGTT10.41841004184100417No Hit
CCCTTTGGCTGGCAGGGCATCTTTGCCGGATTGCCATTTGCCGTATGGTT10.41841004184100417No Hit
GCTGACAGCTATGAAGAAGACCTCCTGTTTGTCACGCTGGACCCGATGAC10.41841004184100417No Hit
GCGCCGCGCTGTACGCGCTGACCGCGCTGGAGTACCTGCGCGCGCCGTGA10.41841004184100417No Hit
CTCTTCCAGTCGTCCTTCCTTTACCAAATGGTTTTTTAAAACATCAACTT10.41841004184100417No Hit
GAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGCAAGCAGTGGTA10.41841004184100417No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.41841004184100417Illumina Single End PCR Primer 1 (100% over 25bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.41841004184100417No Hit
GAGTACATGGGATACGGGCACCGAGATCTACACTCTTTCCCTACACGACG10.41841004184100417Illumina Single End PCR Primer 1 (96% over 33bp)
ACGATTGCCAGTACCGCCAACAGCGTCGACGTGGGCGGTACAGCCGGGGC10.41841004184100417No Hit
GGATAGGAGAGAGATTGTAGGCAAAGAAACAATATGTGCAAGTTCTCAGA10.41841004184100417No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.41841004184100417Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGATACCGAAGAGATCTACTTTTCCCAGCAACTCCAGTCCTT10.41841004184100417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCGTCTTT10.41841004184100417No Hit
CTACCTTTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
AACCTCAACCAGGCTCTCGGCAAGTTCGCTGCGGCTGGCTGGATGATCGT10.41841004184100417No Hit
ATGCCGATGGGCTCGCCGGTCTCCTCGCAATAGCCATAGCTGCCGTCCTC10.41841004184100417No Hit
AGGGAAAAGTGACTCGTGTTCCCATGTACTGCGTTGATACCACTGCTTCC10.41841004184100417Clontech Universal Primer Mix Long (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAAT10.41841004184100417No Hit
GTTCGCGAGGCGCCCCGCTGTCAGGCCGTCGATCGGCGAAAGCTCTGTTT10.41841004184100417No Hit
CCATAACACGATCTAGTGAAACCTTCAAGCATGAATTGTAGCTGACGGCA10.41841004184100417No Hit
ATCAACGCAGAGTACATGGGTTCGGCGACCACCGAGATCTACACACTTTA10.41841004184100417Illumina Single End PCR Primer 1 (96% over 27bp)
CCGTTCCTGAGCACCATGGTTAACTTGATTTGGTGTTATTCAGCATGGTT10.41841004184100417No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGGGGGTTTT10.41841004184100417No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.41841004184100417Clontech SMART CDS Primer II A (96% over 26bp)
CCGTATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTATCATCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATC10.41841004184100417No Hit
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.41841004184100417Clontech SMART CDS Primer II A (95% over 23bp)
CTCGTCTTTCACGAGATCCCACAGCTCGGCCCCGCGGCGTTCGAGGAGGT10.41841004184100417No Hit
GAGGCAGTGGTTGCAGTGAGCTGAGATCACGCCATTGCACTCCAGTCTGG10.41841004184100417No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.41841004184100417No Hit
GGGATTGCTAACTAATTTTAATATTAATCTAAGAAATACACAATTGAGAT10.41841004184100417No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGTGTATCTTGGCTATCCCATGT10.41841004184100417Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTTCTTAGTGGTGAGTCAGGAACAAATATTTTGTAGGAGACACGTTAAG10.41841004184100417No Hit
TCCTCCACCAGCGCGCCGAGATCGTCGCGCATGCGGGCGCTCTCGCCGTT10.41841004184100417No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGC10.41841004184100417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA10.41841004184100417No Hit
GAGTACATGGGATACGACCACGAGATCTACACTCTTTCCCTACACGACGC10.41841004184100417Illumina Single End PCR Primer 1 (96% over 31bp)
AGAGAAAACAGATGAACAACATAGCTTAGAAATATTGAAAAAAAAAATAA10.41841004184100417No Hit
GCATTGCCCGCGATAGCGGCCGGGGGTGTGGGCGCGGGAGGGCGCGTGCG10.41841004184100417No Hit
GTGGTATCAACGCAGAGTACATGGGATGATACGGCGACCACCGAGATCCT10.41841004184100417Illumina Single End PCR Primer 1 (95% over 24bp)
GCATTTATAAGACAGGCAATATTCATTCTTGAGAAAAAAAAAAAAAAAAA10.41841004184100417No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.41841004184100417Clontech Universal Primer Mix Long (96% over 26bp)
GCAAGTAAGGGCTTGCTCCCCAGAGCCCCATGCCACCCATGTAGGTTTCT10.41841004184100417No Hit
GGACATGCGCGACACGCTGAAGCTGCAGCCGTGGCTCAACGATCCCGACC10.41841004184100417No Hit
GTGTTTCTTGTAAGGAGGCTCTGATGGCAGCTTCTAGCTGGCTGTCTTCA10.41841004184100417No Hit
CTACACTCTTTCCCTCACGACGCTCTTCCGATCTCAGACCATGATGCGGA10.41841004184100417No Hit
CACTCTGTTGACCAGGCTGGAGTGAAATGACACTATCATGGCTTACTGCA10.41841004184100417No Hit
CCTGGGTCACGACCCTGGGCATCGGGGACCTGATGCTGGACTTCTGGTGG10.41841004184100417No Hit
GCCTCGTTCCGCGGCCTGCACGATTCCCTCGTCAACTGGCGCGGCGGCGG10.41841004184100417No Hit
GGTATCAACCGCAAAAAAAAAAAAAAAAAAACAAAAAAAAAAATAAACGT10.41841004184100417No Hit
CTCTTTCCCTACACGACGCTCTCCGATCTCTGTCCCAAGTTCTGCAGGTA10.41841004184100417Illumina Single End PCR Primer 1 (95% over 24bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA10.41841004184100417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGGAAATCTAACTTCCCTACACGACGCTCTTCCGAT10.41841004184100417Illumina Single End PCR Primer 1 (96% over 25bp)
CTCTTCCGATCTCATGTTTACTGGTATCGGCAGAGCGTCGTGTAGGGAAG10.41841004184100417Illumina Single End PCR Primer 1 (95% over 24bp)
AGAAGGCTCCGGCCAAGAAGGCCGCTCCGGCGAAGAAGGCTCCGGCCAAG10.41841004184100417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)