FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT381.821668264621285Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA331.5819750719079577Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.6711409395973155Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120.5752636625119847Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG100.4793863854266539Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90.4314477468839885Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG90.4314477468839885Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.38350910834132307Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC60.28763183125599234Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG60.28763183125599234Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG60.28763183125599234Clontech Universal Primer Mix Long (96% over 26bp)
CGGGCAGACTCACCGACACGCCAAGACCCCCCTGGAGGCAGCTTTCCACA50.23969319271332695No Hit
CTCTTTAGCAGATTGCTTGTGATGCACATCACATTCTGGTTTAATGCCTA50.23969319271332695No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.23969319271332695Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.19175455417066153Clontech Universal Primer Mix Long (95% over 21bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.19175455417066153Clontech Universal Primer Mix Long (96% over 26bp)
TAGTAGAGGTGGGGTTTCACCATGTTGGCCAGGCTGGTTTTGAACTCCTG40.19175455417066153No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG40.19175455417066153Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.19175455417066153Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40.19175455417066153Clontech SMART CDS Primer II A (96% over 26bp)
ATTCAAGCGATTCTCCTGCCTCAGCCTGGATTCTTAAATCAAATCACAGT40.19175455417066153No Hit
GTGTTCTTTCTGTCTTTAGTTTTTTAAGTAGAGATAGGGTCTTGCAGTGT40.19175455417066153No Hit
GCTGGCAGCGCTGGAAACTGGGTGGACGGCATGGGTTGGTCTCAGGATTT40.19175455417066153No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40.19175455417066153Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT40.19175455417066153Clontech SMART CDS Primer II A (100% over 26bp)
AGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTAAGTGAATTAGCAG40.19175455417066153No Hit
GGGAGGCCGAGGCGGGTGGATCACAAGGTCAGGAGTTCAAGACCAGCCTG40.19175455417066153No Hit
TAGCATATTCGATCGTATTAAGACTGCTTAGTCGGCCGGGCGCGGTGGCT40.19175455417066153No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT40.19175455417066153Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.19175455417066153Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT40.19175455417066153Clontech SMART CDS Primer II A (96% over 26bp)
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA30.14381591562799617No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAA30.14381591562799617No Hit
CCTCCAACCCGCCGTCTGGGCGGGGTGCCAAGGTGACGGTGAACTCTCGG30.14381591562799617No Hit
CCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAAC30.14381591562799617No Hit
TAGCTACTCAGTGCTAAAATGGGATTATGACTCTAAATCCCACTGTGCTT30.14381591562799617No Hit
GTGCTGGTGCGTCTGGTAAGCATGGCAGGTGGTGAGCGATACGTCACCGA30.14381591562799617No Hit
GTAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCAACAGAGAGA30.14381591562799617No Hit
CACACGATGTTGTCAAGGATGGTTTTGGGACTAGAGGCTCAGTGGTGGGA30.14381591562799617No Hit
TCCTGTAAACCTGGACAAAGACCGCAAAAAGATCCTCGAACGGAAAGCCA30.14381591562799617No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.14381591562799617Clontech Universal Primer Mix Long (96% over 26bp)
CGTTCTGTAAAGAACTCATTTCTCTGGACCAAATTCAAATACTGTCTGAA30.14381591562799617No Hit
GACTGTTGCTGTTTGTGTGCTGTTGTTTGAAGGTGGATTGGGCTTTCACG30.14381591562799617No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC30.14381591562799617No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT30.14381591562799617Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTGAGCAGGGAATAAAGCACCCTCCTGAGGTGCCCAGTTCCCTCAGA30.14381591562799617No Hit
TCTTAGAAGAGTACTTGAGCTCCACTCCGCAGCGTCTGAAGTTGCTGGAC30.14381591562799617No Hit
TTGAGAAGCTCTTCGCCATGGACACAGAGGCCAAGGACCAATGCAGTACC30.14381591562799617No Hit
GCCCTAGGCCAGGGTGAGAATGGAGATGTAAAGTGGGCATTGTAAATACA30.14381591562799617No Hit
AGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGC30.14381591562799617No Hit
GTATGTATATTTTGTAAATCTCCCATTTTTGTAAGAAAATATATATTGTA30.14381591562799617No Hit
GCCTTTCCCTACTTCTGCTGCGCCTGCGTTCTAACCCCCGGTCAATACTT30.14381591562799617No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.14381591562799617Clontech Universal Primer Mix Long (95% over 22bp)
GGGGTCTGCCCAGAGTGTTGGCCTTAGCTAAGACCCAATAAGCTATTCCT30.14381591562799617No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT30.14381591562799617Clontech SMART CDS Primer II A (100% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT30.14381591562799617Clontech SMART CDS Primer II A (96% over 26bp)
CCACGGGTGACGGGGAATCAGGGTTCGATTCCCCGTCACCCGTGGTCACC30.14381591562799617No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.14381591562799617Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.14381591562799617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC30.14381591562799617No Hit
GCTAACTCCGCACCCAAAATTCCAGCCGACATATTGGGCGGTAAGGTTTG30.14381591562799617No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.14381591562799617Clontech Universal Primer Mix Long (96% over 26bp)
CATCTGCTCTTCTAAAGGAAAGTTCAACTCTATGAGTTGAATACACACAG30.14381591562799617No Hit
GGTAGGAGCATGATGGAGGCTTCGCTTGAGGGCCTGGGAGATGCCGCCGA30.14381591562799617No Hit
ACTGATAACATTGTTACAGAAGAATGGCAGCTTACTCCAGGGCACTTCAG30.14381591562799617No Hit
TTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAA30.14381591562799617No Hit
GTACATGGGAGTACGGCCACCGAATCTACACTCTTTCCCTACACGACGCT30.14381591562799617Illumina Single End PCR Primer 1 (96% over 28bp)
CATTTAAAACAACCAGAAATCACGGGTAATATGGAATGTTTATGTAGCAA30.14381591562799617No Hit
GTTCCACGAGGGCGACGAGCCCGAGGACATCGCCGCGCAGCTCGCCTCCG30.14381591562799617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14381591562799617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)