FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82.4096385542168677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG61.8072289156626504Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51.5060240963855422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51.5060240963855422Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT41.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGGTA30.9036144578313252Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGGCCTG30.9036144578313252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAGAAAAAAAAAAAAAGATAGATGGCTACCCCTGACTCCCATT30.9036144578313252No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30.9036144578313252Clontech Universal Primer Mix Long (96% over 26bp)
ACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCC30.9036144578313252No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.9036144578313252Clontech SMART CDS Primer II A (96% over 26bp)
GTCCGGCCCCCCTCTTCATAACGTGCATGCACCCGGACGGCCCCATCACG30.9036144578313252No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.9036144578313252Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAAATTCCTAGGTTTTTGGCTTGAGCA20.6024096385542169No Hit
GAATCTTTGAAATGTATGCAAATATTTTTTAAGCCAAATAAACCTCTTGC20.6024096385542169No Hit
CAGCATAACTTCTATTTATTTCCTTTTTTTTGTTGAAAATTTTGTTTTAA20.6024096385542169No Hit
AGATGGATTGTAGGGAGTAGGGTACAATACAGTCTGTTCTCCTCCAGCTC20.6024096385542169No Hit
GTATTTGTTCTGTTGTCATAGAAACCATCATTAAAATAATTCACCAATGC20.6024096385542169No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC20.6024096385542169Clontech Universal Primer Mix Long (96% over 26bp)
TAAAGCACCTGTGTCTTTGGAGAACTCCATTGAAGAAATATTAGTTGAAT20.6024096385542169No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT20.6024096385542169Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGGAAGAGTTGCCATGCTCTTTTGAATTTACATAAATTCTTAACATC20.6024096385542169No Hit
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.6024096385542169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTCATTGCCGACGGCGGCGACGGCCTTGGCCAGCGCCGCGATATCCTCA20.6024096385542169No Hit
GCCTCAACCTCCCGACCTCAAGTGATTCTCCCACCTCAGCCTCCCACGTA20.6024096385542169No Hit
CCTGCATATGTTAAACCACCCCTGCATTCCTGGTTTGAAACTTACTTGAT20.6024096385542169No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.6024096385542169Clontech Universal Primer Mix Long (96% over 26bp)
TAGGGAACCTAAAATGATAAGATGGGCAAATGTTCATTTACCTTTTTCTC20.6024096385542169No Hit
GAACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTC20.6024096385542169Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTACGGCGCCACGAGATTACACTCTTTCCCTACTGTCTCTTATACAC20.6024096385542169No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.6024096385542169Clontech Universal Primer Mix Long (95% over 21bp)
GACCTGGGCGAGTTTGTCCTTGCCGCTCTCGAGCTTGAGGAAGGTCACAG20.6024096385542169No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.6024096385542169Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20.6024096385542169No Hit
AACGGCCACACCCGACAAGATCGACGGACTAAACCAGCGGCTGGCGGAGT20.6024096385542169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.6024096385542169No Hit
CTCCTTCAGCCCATCCACTCTCCTCTAGAGCAGTTGGGTTGGAGGCTCAT20.6024096385542169No Hit
ATCCTGCCCTGAGCTTCATAGTCCTCCTCAGTCAAGTGGGCATCACAATT20.6024096385542169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.6024096385542169No Hit
ACCCCCCTCCATACCCAGTTAACATATTCTAGGAAGTTGCTTCACCACAC20.6024096385542169No Hit
GCCCAGCCATTCGTGACATGTTTATACAAATATAACTTTAGCAAATATTT20.6024096385542169No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC20.6024096385542169Clontech SMART CDS Primer II A (100% over 26bp)
AGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTA20.6024096385542169No Hit
GTGGTATCAACGCAGAGTACATGGGATGTAGGGCCACCGAGACTACACTC20.6024096385542169No Hit
TTGGTGAACAATCTACTTGCCAATGAAATTTTTAACTCCACCTAGATGAA20.6024096385542169No Hit
GTGCTTTATCTTAGTTCTCCCACTACCATCTAACTTGCCAAAATTATTTC20.6024096385542169No Hit
AGTACACTCTTTCCCTACACGACGCTTGCCTCAGATTCTGTCGGTACCAG20.6024096385542169Illumina Single End PCR Primer 1 (95% over 24bp)
TCAACGCAGAGTACATGGGGGGGACCAGATGGATTGTAGGGAGTAGGGTA20.6024096385542169No Hit
CTGCTGAATCACACGCCGCCATCCGGGAGGCCCAGGGAAGGTGCAATGGA20.6024096385542169No Hit
CAACAAAGTAAGTAGGCAAAAGGTTTGTAAACTACACTTACTATTCAATA20.6024096385542169No Hit
GAATGATGGTGGTCTTTTGATGGACATTGCATTGAATTTGTAGACTGCTT20.6024096385542169No Hit
CTACTCAGGAGGCTGAGGAAGGAGAATTGCTTCAGCCCAGCTGCAGTGAG20.6024096385542169No Hit
GTTGTGCTCGACGAACACGGCCGGCACATCCCGTCCCGGCCGCCTGCCGA20.6024096385542169No Hit
CTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGCGCCCGCCTGTAGTC20.6024096385542169No Hit
GATACAAGATCAGTACATATAAAACAATAGTATTTCTGTACTCTAGCAAT20.6024096385542169No Hit
CAGAACGCCTTTCCTCTTGCAAAACTGAAACTCCGTAGTCAGTAAGCAAC10.30120481927710846No Hit
ACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GCACAAACCTGCTTCTTTTTTCTAGCAAGATGCCAAATTCTCCAGCACAT10.30120481927710846No Hit
GTTTTCTCACAGAGGAATTTTGATTGATACATCCAGGCAGATAATGTCTG10.30120481927710846No Hit
GCAATATACTTAAAAGGATAAGTATATTGCACATGTTTATTTGGCAAAAG10.30120481927710846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACCC10.30120481927710846No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10.30120481927710846No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
ATTGAAGAATGTGCTAATGCACTCATACCAAAGAGCCAGAATTAAATTTT10.30120481927710846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GCATATGCATGCCACCATGCCCGGCTGTTTTTTTGTTTGCTTGTTTTTGG10.30120481927710846No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTATATTATAGTTTGAAGTTCAGTAATGTGATGCCTCCAGATTTGTT10.30120481927710846No Hit
TACGTAAGCTCCCAGGTCCTGGGAAAACTCAGGTCATGCACGTTGAACTT10.30120481927710846No Hit
GCTACAGGAAAACAGAATTTCTTGCACCACTAACCAAAGAAATAAATACA10.30120481927710846No Hit
GGCAATGCTCTATCCGCTCAAAGCCACGCGCTTTGGCCTCCAATTGGTCA10.30120481927710846No Hit
TCCTTGAGCCACTCGATCCGTCGCTCCAGCGAGCACAGCAAGGCCGGGGC10.30120481927710846No Hit
AAATAAATGTTCCTTGTTGAGGTCACCCAGTTTACAGTATTCTGTTCCAG10.30120481927710846No Hit
CCGCTGGTGGCGAACAGGGCCGACTGGGCTAGGTTCACGCCCTCGTCCTC10.30120481927710846No Hit
ATCTGAGCGCGGTCGGCCCCGACGGGCAGCGATCGGTGTTTGCCGGCGCC10.30120481927710846No Hit
CCTGGGAGGCAAAGGTTGCAGTGAGCCAAGATTGCGCCACTGCCCTCCAG10.30120481927710846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTAACCAA10.30120481927710846No Hit
GGCATACAGGTTGAGCAGCTCGGCGGCGGTGTCGCGCACCTGCTGCGCGG10.30120481927710846No Hit
CTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCC10.30120481927710846No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATTATGCGTTGAAA10.30120481927710846No Hit
AAACAAGACCCGCTGAGGCGCCCAGCGGGCAGCGTCCCCCAGACGACCCC10.30120481927710846No Hit
GCAGGAAGACCTGGCAACGGATGACGTCATGCTTCTGGACACCTGGGACC10.30120481927710846No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA10.30120481927710846No Hit
AGCTGGGTGTGGTGGTGCATGCCTGTAGTCCCAGCTATTAGGGAAGCTGA10.30120481927710846No Hit
GACTGATTATGATAACTTGTCAAATTTTGGTATTTTAGGCCAAGTAAAAG10.30120481927710846No Hit
ATTTTTCACTGTGTGCCAGGGAATTTCAGGTCATCACATTTACCCATTAT10.30120481927710846No Hit
TTTATTAGCTTTCCATAAAAACACATAACATACACAATCTACAGTATAGA10.30120481927710846No Hit
GATACCACTGCTTCCCAAGTACTCTGCGTTGATACTGTCTCTTATACACA10.30120481927710846No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTC10.30120481927710846No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGCGTTGAT10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGTAAAGCGGTATGGCGACAAGATGGAGAACCTTGGCTGTTGGTACGAC10.30120481927710846No Hit
TCAACTACCTGCGCAAGCGGACGGTGCGGCGTTGCCAACCGCCGCGTGAG10.30120481927710846No Hit
GCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCG10.30120481927710846No Hit
TATATAGCCAGGTGGCACGCCTGTACTCCCAGCCACTCGGGAGGCTGAGG10.30120481927710846No Hit
GAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCAGAACGACACCCGTACGGCCGACCCCGCCCGCCTGGACAAGGTGGT10.30120481927710846No Hit
GGGTTTCACCGTGCTAGCCAGGATAGTCTCGATCTCCTGACCTCGCGATC10.30120481927710846No Hit
CTTCCCTTATTTTTTATAAGGGCTTAGACAGTCTTCTAATTTCTTTGTTT10.30120481927710846No Hit
ATGTCATTGCCTTGGAAGAACAGCGAGTGGCGGGCCATGATCTGGCGCAT10.30120481927710846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAGAAAGG10.30120481927710846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATCGTTGATAACAA10.30120481927710846No Hit
CTTGTGATCCATCAGCATCCCAAAGTGCTGGGATTACAGGCGTGAGCCAC10.30120481927710846No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC10.30120481927710846No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATTTAAA10.30120481927710846No Hit
GCCGATGAAGTTCAGCTGGATCGCCTGCCAGCCGAATTGCCGCGGCTGGG10.30120481927710846No Hit
CATTAGAAGATACCACATGTAAAGTTAATGATAGTGCTTGTTACTTAGTA10.30120481927710846No Hit
GGAAAGAAGGGAGCTTTCCCTTAAGTGCAAAAGATATGGTTGGGAGGTTG10.30120481927710846No Hit
CCAAAGCTCCCATTGGTGTAATTTGCAGCACTGTAACTGTCTCTTATACA10.30120481927710846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCAAGGAGTACC10.30120481927710846No Hit
CTCCTGAGGTGGGCAAGTAATCTGTCCACCTCAGGAGTTCGAGAAGCAGC10.30120481927710846No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTAAAAA10.30120481927710846No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGTTCCT10.30120481927710846Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAA10.30120481927710846Clontech Universal Primer Mix Long (96% over 27bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CTGTAATATGAGTAATTTGTAAAACAGGAAGTTGGTCAGCTCACTACCCT10.30120481927710846No Hit
GAAAAAACTATGGGCCAATATTCTCAATGGACATAGGTGCAGAAGCTCTT10.30120481927710846No Hit
TACGTAAGCTCCCAGGTCCTGGGACAACTCAGGTCATGCACGTTGAACTT10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.30120481927710846Clontech SMART CDS Primer II A (100% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCA10.30120481927710846No Hit
GCCTAGCATGTCCTCTGCACGCCCCGCCGTACCCTGACGCGCGCCGCAGG10.30120481927710846No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGTACATGGGAA10.30120481927710846Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATTTACACTTTAACTACAAACCAAGAGTCTTGGCCAAGCACAGTGCCTC10.30120481927710846No Hit
CTACAGTAGTCCCCCTTATCCAGTTTCTCTTTTCCCAGTTTCAGTTAGCT10.30120481927710846No Hit
TATTAAGTGCTTTGTGCTTTTTTTTTTCCCCCTGAAACAGTCTTACTCTG10.30120481927710846No Hit
CCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTTCTTCTGCTTCCTACTT10.30120481927710846No Hit
GTGTCATCCCTGGTTGAGCTGGATCTGTCCTATAACAAGCTTAAAAACAT10.30120481927710846No Hit
CTATTAAGCAAGTCACAGGTCAAATGCCATCTCATCTGGAAATTGTTCCT10.30120481927710846No Hit
GTACATGGGAAGTACGGCGACCACCGAGATCTACACACTTTCCCCTGTCT10.30120481927710846Illumina Single End PCR Primer 1 (96% over 25bp)
GCTCTCTACAGCATCTTGAAGCTGCAGCATCTTTGCAGATGCCCCCTTTA10.30120481927710846No Hit
GGATAAGGCAGGAGAATCACTTGAACCTGGGAGGTGGAGGCTGCAGTGAG10.30120481927710846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAGCCACAATTT10.30120481927710846No Hit
GGATTTGTCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACC10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGGCAGCGTCCCCTGGTTCAGGTTGTCGAACCCTTGCCAGTTGGCCA10.30120481927710846No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG10.30120481927710846No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG10.30120481927710846No Hit
GATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCTGTCT10.30120481927710846No Hit
ATCCTTGATCGCGCTGGGGAAACGTTGCACAACGCCCGGCGCACGGCCAC10.30120481927710846No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAAAAAA10.30120481927710846No Hit
GCGCCTGCACGGTGCTGATCGATGGCGTGGCCGCGCGTTCCTGCGTGATC10.30120481927710846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT10.30120481927710846No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATC10.30120481927710846Clontech Universal Primer Mix Long (96% over 25bp)
GACTTAGCTCAGTGGTTCTGAAAGTCTAATTTTGGATAGCATCACTTGAG10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGG10.30120481927710846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGACGTGTGCTCTGCGTTG10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.30120481927710846Clontech SMART CDS Primer II A (96% over 26bp)
GGATCAGTAGGATCCTCCCTTGCTACCGTCTAAAGATCTGCCGCGTGCTG10.30120481927710846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACCTGTCTCTTATA10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAAGGAAGGGAGGAGAAATTAGGATTGAGATAGATGAGATAGGAAA10.30120481927710846No Hit
TTACTAGCATCTCAAACAGCTCAGCCCTTAGCTGCCCAGGTATACTGTCT10.30120481927710846No Hit
GGCAGCTGGGAGGTGGAGGTTGCAGCCAGCCAAGATCACGCCACTGCACT10.30120481927710846No Hit
CTTTCTCAAACTTGTGGAGAACGGCTTTCCTCTTTCCTGTTTTCCTTTAT10.30120481927710846No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
TGGTCATCTTGCCCTGGGCGGCGCTGCCCATGCCGTAGCCCTTCTTGGTG10.30120481927710846No Hit
GGATCTCAGTCTCACCCAGGCTCGAATGCAGTAGTGCAATCTTAGCTCAA10.30120481927710846No Hit
ATTCTGTAATCAGCATAATACTAATCTAGTTAATTTGAGCTTCGGTTTCT10.30120481927710846No Hit
TGGTTGGAGATGTTGGTGTAACCATAACTGATCCTTTCAAGCATTTTGTG10.30120481927710846No Hit
GGTATCAACGCAGAGTACATGGGAGAACGGCGACCACCGAGTCTACACTC10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGTACACTTCC10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.30120481927710846Clontech Universal Primer Mix Long (95% over 24bp)
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTA10.30120481927710846No Hit
TAAAAGGTTCTCAGAGTCTGTTTTGTGAAGAGATCCCTTAAAGTATAATG10.30120481927710846No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
TTCTAAGACTCATGAAAGCAAGCAAAGATTTACACACTCCCTTCATTCTT10.30120481927710846No Hit
GGTGAAAACCTCTCTTTGGAGGAGGGCGTAGCCAGAAACCTCATTAATCC10.30120481927710846No Hit
ACATAGACCCCTCAGGAAGTTGGGGTCTTCCCTACTTGCTCCTAGCCATA10.30120481927710846No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA10.30120481927710846No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
ATGCTTTATCTTAGTTCTCCCACTACCATCTAACTTGCCAAAATTATTTC10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGTACGGCG10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAATGATACGGCGACCCGAGATCTACCTGTC10.30120481927710846No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGG10.30120481927710846Clontech SMART CDS Primer II A (100% over 26bp)
ACGTAGCAGCCCTTGGCGAAGCTGACCCCGTTCAGTTCGCGCGCGTTGCA10.30120481927710846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTCGTTTATAACAA10.30120481927710846No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
AACTGAAAATGCATTGATCGATTTGAACTTCCTGGGGATAGATAACGTCT10.30120481927710846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.30120481927710846Clontech SMART CDS Primer II A (100% over 26bp)
CCTCCACCTCATGGGTTCAAGCAATTCTCGTGTCTCAGCCTCCAGAGGGT10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC10.30120481927710846No Hit
AAATAACCAAAATTCGTTAGAGAACTGAATGAAATTGAGACTCAAAAATA10.30120481927710846No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGCGGAG10.30120481927710846No Hit
GTGTGGTGATGCGTGCCTGTAGTCCCAGCTGCTTGGGAGACTGAGGCAGG10.30120481927710846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.30120481927710846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAACTGGCTGATA10.30120481927710846No Hit
GGAATGATACGGCGACCACCGAGATCTATACTCTTTCCCTACACGACGCT10.30120481927710846Illumina Single End PCR Primer 1 (97% over 48bp)
CTCAATTATGTAAAATTCATTTTGAAGAGAATAGGAGGAAATCTGCTCCG10.30120481927710846No Hit
AAAGAATACCTAGGACTAGGGTAATTTATTTAAAAAAAAGAAGAGGTTTA10.30120481927710846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAAAACACA10.30120481927710846No Hit
GCGCCCGACGTCGCGGACATGCTCAATCCGCTGAAGGGCATGGCCGACCG10.30120481927710846No Hit
ACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.30120481927710846Clontech Universal Primer Mix Long (96% over 27bp)
ACACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGT10.30120481927710846No Hit
TTCCAGCCTGGGCTACAAGAGCAAAACTCTAACAACAACAACAACCAAAA10.30120481927710846No Hit
CTCTTTCCCTACACGGAGCTGCCGATACCAGTAAACATGAGATCGGAAGA10.30120481927710846No Hit
GACATATAGTCATCAGATTGCTTAAAGTCAGTGTGAAGGAAAAAATTCTA10.30120481927710846No Hit
ATCTTCAGCATATCGCTTTTGTAAACGTGCTTGTTGAATCTTTAAATTTT10.30120481927710846No Hit
GTTTACCGTGTGGATATGTGAATTGCAATAAATAACAGGCTCTCACGTTT10.30120481927710846No Hit
GGCCAATACGGGCCGCTATCGCTTCATTCAGCTCGACATCCAGTTTTCCG10.30120481927710846No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.30120481927710846Illumina Single End PCR Primer 1 (100% over 30bp)
AAACGAAATGGTGTGTAGCAATCAACACTAGAAAGTAGACCTTTTGCAAA10.30120481927710846No Hit
GCGTCAGATGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGGGGGT10.30120481927710846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACGACTAA10.30120481927710846No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTGCATGG10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA10.30120481927710846No Hit
TCTGCTTTCTCTTTGATTCCTCCCCATTCCAGTAGCATTCCAGATAGCGA10.30120481927710846No Hit
AACCACCCCTTCCGCTGGCGGGTGCACTGCGACGGACGCACGGTGGCCTC10.30120481927710846No Hit
ACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAG10.30120481927710846No Hit
GGCGATGGAGGATGCGACAGGGCGTTGACTTGTCTGCCCCCCTTTGTGAG10.30120481927710846No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.30120481927710846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAATAAAAAAAAA10.30120481927710846Clontech Universal Primer Mix Long (95% over 21bp)
GGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAG10.30120481927710846No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATAC10.30120481927710846Clontech SMART CDS Primer II A (96% over 26bp)
GTACCGTCTTGACCGGCGCCGGATTCGGGAAGCTGAACATCTGGTGGATC10.30120481927710846No Hit
CTTGATGTACTCAACTTCGTTTTGTATGGTGAGGATTTTTACTGATAGGG10.30120481927710846No Hit
TTTCTAAACTGCTCAATTTTGACTCAAAGGTGCTATTTACCAAACACTCT10.30120481927710846No Hit
CTCTTCTCGGCCGCCGCCGGCAAGGCGGCGAGCGCGGCAAGGAGCTGTCT10.30120481927710846No Hit
CTTTCAACATACATTCCCTCAGGAAATGTATGTTGAAAGAATTGATTAGG10.30120481927710846No Hit
GAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGC10.30120481927710846No Hit
CGGCAGCTTCACGCCCGCAGTCCGGGCGCTGTCTCTTATACACATCTGAC10.30120481927710846No Hit
CGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGG10.30120481927710846No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTC10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCTGTCTCTTATAC10.30120481927710846No Hit
TGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCC10.30120481927710846No Hit
GTCTATGGGGAAACATGACGTGTGATGCAAACTTCTTAGCTGGGTTGCAC10.30120481927710846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.30120481927710846No Hit
GGTATCAACGCAGAGTACATGGGCAGGTTAAAATAATGGGTTATAAGATA10.30120481927710846No Hit
CTATTGAACCAAACCCAACGAAGAAAAGTCTGCTAATAAAAGCAATAATC10.30120481927710846No Hit
CATGTGGAAGATCTCGCCCTTGCCGCGCGCGGCGGCCGCCACGCTGATGA10.30120481927710846No Hit
GAGAGCGAAAAGGCGAAGACGACGCAGAATCCAATCCAATCCAAGCAATG10.30120481927710846No Hit
GAGTACATGGGAAGCAGTGGTATCAACACAGAGTACTTTTTTTTTTTTTT10.30120481927710846Clontech SMART CDS Primer II A (96% over 26bp)
CTAATTAATGAACCCCTCTTTATTCCTTCTCCCCACACCTGTAACTGTCT10.30120481927710846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTAAAACA10.30120481927710846No Hit
GCCATCGCCTGCCGCGACATCGGCGTGCGCTTCTTCACCGAGCGCCGCGA10.30120481927710846No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAGAGTACAT10.30120481927710846Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTTTTGAGAAACACACAGGAACTCCAAAGACAGGGTCTCTGCTCCCCTT10.30120481927710846No Hit
GTGCGGTGGTACATGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGG10.30120481927710846No Hit
TTTTCGAGATCATCGGGCGGGCGCTGGCGGAGGCCGGGGCGTCGTTCGCG10.30120481927710846No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGATGACGGCG10.30120481927710846Clontech Universal Primer Mix Long (96% over 26bp)
CGGGTCGACGCCGAAGTTGTCGAGCAGCGGGATGAAGATGGGCATGAAGA10.30120481927710846No Hit
CGATATGACAAGTTAACAAGACGGCGCACAAGCACGATCGGCTCCTAATA10.30120481927710846No Hit
GTACATGGGGACGCGAGTCCTTTCCCTACACGACGCCTTCCGATCTGGTA10.30120481927710846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)