FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCAGAGTACCCGGTTTGGTGCTTTCCTTGACCCTGTAGCAGATAAAG33.75No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33.75Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAGCGCCGCCTGCTCGACCGCGGCCATTTCCAGCCCGACCTCCCGCC22.5No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGAAAAAA22.5Clontech Universal Primer Mix Long (95% over 22bp)
GTAGGGAAAGAGTGTAGATCTCGTGTCGGTATCTTCCCATGTACTCTGCG22.5Illumina Single End PCR Primer 1 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22.5Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGACCCCACTAGCCCATAGCGATCACTTTCTGACTTTTGGTGGCAA22.5No Hit
GATGACAAGAGAGAGAAAATCACTTCTTCCTGCTTCACAGCTTCATCGTA22.5No Hit
GTACATGGGAGACGACCGAGATAACTCTTCCCTACACGACGCTCTTCCTG11.25Illumina Single End PCR Primer 1 (95% over 22bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAACGG11.25No Hit
TTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGATCTGCAGGTACCA11.25Illumina Single End PCR Primer 1 (100% over 26bp)
TGGTCACCTCGTCGTAGCAGTGGCGGATCTCGGCATCGATCGCCTTCTTG11.25No Hit
TCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAA11.25No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCACCCATGTACTCTGCGT11.25Illumina Single End PCR Primer 1 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA11.25No Hit
GCTGCCGCCCCTGCCGGCTTGCATCGTGCAGGACTACGCTCCCCGGTCAT11.25No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAACTGGGTTTAT11.25No Hit
TATCTACACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATAC11.25Illumina Single End PCR Primer 1 (97% over 37bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTATACCACGGCT11.25No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA11.25Illumina Single End PCR Primer 1 (100% over 21bp)
ATTTCATGCTGTCCGACATTGCGATGAGCGATTTCCGCACCTTCGCGCAT11.25No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGGTAACAA11.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC11.25Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGGAGAATCTCTCCTTCGCGATGTTCTACAACCGCATCGGCTGGGCCGG11.25No Hit
GAGTGTAGATCTCGGTGGTCGCCGTACTTCCCATGTACTCTGCGTTGATA11.25Illumina Single End PCR Primer 1 (96% over 26bp)
GATAGCTATATTGAGACCCGTAGTGTTTCGATGTTTCAAGGCTGTCTCTT11.25No Hit
GGGTGCCTAGCGAGGCGGTGATTCGCACCGGTCAGCGAGCCTTGGTGATG11.25No Hit
GTACATGGGTACGGCGCCCCAGTCAACACTCATTCCTCCGATCAATTGTA11.25No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGT11.25Clontech Universal Primer Mix Long (96% over 28bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.25Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACGGCGCCCCAGACAACAAACATTCCACCGAACACTGGTA11.25No Hit
GTACATGGGAAGCAGTGGTATTAACGCAGAGTACATGAAGCGCTGTCTCT11.25Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTATCTCGGTGGTGCCGATCAACCATGTACTCTGCGTTGATACCACTG11.25Clontech SMART CDS Primer II A (95% over 23bp)
GTGTAGGGAAAGAGTGTAGATCTCGGTGGCGCCGTATTCCCATGTACTCT11.25Illumina Single End PCR Primer 1 (96% over 29bp)
AAACAAAAATATTTACCAACACGACCATCTACACATCTAAACAATATTAT11.25No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCAC11.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAACGGCGGGA11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAGGGTTTTAAAACCCC11.25No Hit
CCCCGAGATCTACACTCTTTCCCTACACGACGCTTGCCTCAGATTCTGTC11.25Illumina Single End PCR Primer 1 (97% over 34bp)
CTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCGGAGAT11.25Illumina Single End PCR Primer 1 (96% over 25bp)
GTACATGGGTGTACGGCGACACCCAGTAAAACTCATCCCAACACAAAGCT11.25No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA11.25Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGCTGTCTCTT11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGCCTAGCGAGGCGGTGATTCGCACCGGTCAGCGAGCCTGGGTGATG11.25No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCAC11.25No Hit
GTTTTCGGCGGGTAACCTGTTCCAGGTTATGCGCCCTACGGTCCTGACCC11.25No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTATGCGTTGAT11.25No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT11.25Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT11.25No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT11.25Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACA11.25Illumina Single End PCR Primer 1 (97% over 34bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTATAAAC11.25No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATACA11.25Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGAAGAAACCTCGTCTCTACTAAAAATACAAAATTAGCCAGGCGTGGTGG11.25No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGCGCGTACACCCATGTACTCTG11.25Illumina Single End PCR Primer 1 (96% over 29bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.25No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.25No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTCTCTTATACA11.25Clontech Universal Primer Mix Long (96% over 26bp)
TCATGTGGGCGGTATGGGTCAAGCCAGAAGATAGCTGGCATGCCTGAGTT11.25No Hit
TCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC11.25Illumina Single End PCR Primer 1 (96% over 31bp)
GTTTTCGCTCGGCTTGGTGTTTGTCAACGAGGTATTTGAGATCTGCCTCA11.25No Hit
AAACAAAAATATTTACCAACACGACCATCTACACATCTAAACACTATTAT11.25No Hit
ACTCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.25Clontech Universal Primer Mix Long (96% over 26bp)
ATCCCTTTTTTGGTACCAACAGGCCAAAAAAGGGATAGATCGGAAGAGCG11.25No Hit
AGCTGCAGCAGCTCGATGACGCGCTTCTTGATCTCGGCCTCGGGCAGGCG11.25No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTTTATAA11.25No Hit
GGTAAGCTGTACTATAAGAATATGAACACAATCATATTCGTCACGACAAA11.25No Hit
GTACATGGGATTACCGCACGAGATCTACACTCTTTCCCTACACGACGCTC11.25Illumina Single End PCR Primer 1 (96% over 33bp)
GCAATCAACTTGGCGTTGTGTTTCGTACTCGTCCCTTCGCGATTGAGCAT11.25No Hit

[FAIL]Adapter Content

Adapter graph