FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33.75Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCG33.75No Hit
GACAGGTGTCATCCAAAAAGAAGTGCAGCATGGTTTCTTCTTAACTCCCT22.5No Hit
CGCTATATCAAGCGAGATAAATTGACCGAGGCAGAACAAATCTATCTTGA22.5No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA22.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGTGTAGCGACCACGAGATCTACATCTTTCCCTGCTGGTACAGTAG22.5No Hit
TCTTCGGCCAGCATCACCAAGGCTCGCTGACCGGTGCGAATCACCGCCTC22.5No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT22.5Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGCCTGTTGGTACCAAAAAAGGGATAGATCGGAAGA22.5No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGATACCGACACGAGATCTACA22.5Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCCCGGAATGTTGCCATTTATTGAACTGAATGATTGCCGGCCGATGCTCG22.5No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT22.5Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGAAACATCGAAACACTACGGGTCTCAATATAGCTATCCTGTCTCTT11.25No Hit
GGTTCCAGGTCCAAGGCCTGCGCGGCGCTCTTCTGCACGCCCAGCTGAAG11.25No Hit
GTGGTATCAACGCAGAGTACATGGGAATACGGCGCCACCGAGATCTACAC11.25No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCG11.25Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCAACTT11.25No Hit
GTGATATTTGGCGTATGTGTCAAGTCAAAGATGCGCCAATTCGTGACTGG11.25No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTCGTTTTAAACAC11.25No Hit
GTGTAGATGAGGACGAGCAGGGCGAGCGCCGCGCATGAGGCGTCGACGAG11.25No Hit
GTACATGGGAAGCAGTGGTATAACGCAAAAAAAAAAAAAAAAAAAAAAAA11.25No Hit
TTACAAGCCCGCCCTGCTGAACCCCAAGATGAGGCAGATCTCAAATACCT11.25No Hit
GGTATCAACGCAGAGTACATGGGAAGTACGGCGACCACCGAGATCTACAC11.25Illumina Single End PCR Primer 1 (96% over 25bp)
CCCCGAAGGATCGCTGCCTAGCGGCGTCGATCTTGTGGAGCACAAGAAGG11.25No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC11.25Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT11.25Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.25Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGT11.25Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.25Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACA11.25Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAATGTACGGCGACCACCGAGATCTACACTCTTTCCCAACACGAC11.25Illumina Single End PCR Primer 1 (97% over 40bp)
GTATCAACGCAGAGTACATGGGGTACGGCGACCCTCACACTCTTTCCCTA11.25No Hit
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAACAGGGA11.25Illumina Single End PCR Primer 1 (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.25Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA11.25Clontech Universal Primer Mix Long (95% over 21bp)
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACC11.25Illumina Single End PCR Primer 1 (100% over 25bp)
TTACAGGCATGCACCACCACGCCTGGCTAATTTTGTATTTTTAGTAGAGA11.25No Hit
GTACATGGGTGTACGGCCCACCGAGTTACCTCTTTCCCTACACGACGCTC11.25Illumina Single End PCR Primer 1 (95% over 22bp)
GCCCCAAAGCGAGCAGCGTCTCGGCCCCCGCCATGCCCGAGGCCGAATCC11.25No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.25Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA11.25Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCTTATAC11.25Illumina Single End PCR Primer 1 (97% over 37bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATACA11.25Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGTAGACTCGTGGCG11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCATGCTCAATCGCGAAGGGACGAGTACGAAACACAACGCCAAGTTGAT11.25No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.25No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGTGCGGTAATCCCA11.25Illumina Single End PCR Primer 1 (97% over 37bp)
CCGTATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTT11.25Clontech SMART CDS Primer II A (96% over 26bp)
CGCTTCATGTACTCTGCGTTAATACCACTGCTTCCCATGTACCTGTCTCT11.25Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATTACACTCTTTCCCTACACAGCAACTCCAGTCCTTGCATCATGAGTCG11.25No Hit
GAGATACACTCTTCCCTACACGACGTCTTCCGATCTCTGTCCCAAGTTCT11.25No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11.25Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA11.25Clontech Universal Primer Mix Long (95% over 22bp)
CTCCATACCGGCTTTATCAAGCGTACGCCGGAGTGTCGCGACCTTACCCT11.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.25Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGAC11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTTAAACA11.25No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT11.25Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATA11.25Clontech Universal Primer Mix Long (95% over 22bp)
GTCCTTGCGCGAGCTTGCGCCAATGGGTCAGCTGGGCTGCAGCGATCTGC11.25No Hit
GAAGAGCGTCGTGTAGGGAAGAGTTATCTCGGTCGTCTCCCATGTACCTG11.25No Hit
GTTCCGTCACATGACGGTGTTCGACAACGTCGCGTTCGGGCTGCGCGTCA11.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGCGCCA11.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAGC11.25No Hit

[FAIL]Adapter Content

Adapter graph