FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4871
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC210.43112297269554506No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC170.34900431122972697No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC170.34900431122972697No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT160.3284746458632724No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT150.3079449804968179No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA140.28741531513036334No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT140.28741531513036334No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC140.28741531513036334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2668856497639089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2668856497639089No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG120.24635598439745432No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC120.24635598439745432No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA110.22582631903099978No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC110.22582631903099978No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG100.20529665366454528No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT100.20529665366454528No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC90.18476698829809074No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA90.18476698829809074No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT90.18476698829809074No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT90.18476698829809074No Hit
CCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAG90.18476698829809074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.18476698829809074No Hit
ACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGT80.1642373229316362No Hit
TCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCT80.1642373229316362No Hit
GTCTAGTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATC80.1642373229316362No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG80.1642373229316362No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG80.1642373229316362No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC80.1642373229316362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA80.1642373229316362No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT80.1642373229316362No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA70.14370765756518167No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC70.14370765756518167No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC70.14370765756518167No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT70.14370765756518167No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC60.12317799219872716No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC60.12317799219872716No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC60.12317799219872716No Hit
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC60.12317799219872716No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC60.12317799219872716No Hit
GTACATGGGAGAACAACGCAATATATTGCAACCTAAAAATGAAGCTGATC60.12317799219872716No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG60.12317799219872716No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA60.12317799219872716No Hit
GTATCAACGCAGAGTACATGGGGTTACACCCCGCGCAGGATTCGGACGGA60.12317799219872716No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG60.12317799219872716No Hit
CAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCG60.12317799219872716No Hit
GTACATGGGCGGGCATCGCCCCTCGTTTATGAACTAGCCTATCGCAGACG60.12317799219872716No Hit
CTTCTGCTTCTTGGTATCGACATCGTCATCCTCATCATCTTCAGCTGCCC60.12317799219872716No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT60.12317799219872716No Hit
TCCTTGAAGTCCTTTTCGATGGCAGGCGCCACGGCTTCGCTGAAGAGCTG50.10264832683227264No Hit
GTACATGGGAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC50.10264832683227264No Hit
CAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCT50.10264832683227264No Hit
GATGGACGCGGGATTTTTCCGCGGAACAAGTGCAGAACAGGATAATCGGT50.10264832683227264No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC50.10264832683227264No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG50.10264832683227264No Hit
ACGCAGAGTACATGGGCGGGCATCGCCCCTCGTTTATGAACTAGCCTATC50.10264832683227264No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG50.10264832683227264No Hit
GTATCAACGCAGAGTACATGGGAGACCCGCTCATCCCGATCTCCCAGAAG50.10264832683227264No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA50.10264832683227264No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG50.10264832683227264No Hit
GTACTACAGAGACCGGCTGCCCGTGTGCCCGGCAGGTGGAGCCGCCCGCA50.10264832683227264No Hit
TTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTAC50.10264832683227264No Hit
ACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGT50.10264832683227264No Hit
AGGTAGGGACGTGCTGTAGGCCGGAATGTTACCGGCTGTTGGATCTGTGG50.10264832683227264No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT50.10264832683227264No Hit
TGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCC50.10264832683227264No Hit
GTACATGGGCAGAGTCCCTGAACTCTCGCTTTCTTTTTAATCCCCTGCAT50.10264832683227264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.10264832683227264No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC50.10264832683227264No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT50.10264832683227264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG50.10264832683227264No Hit
TTCCACACTTATCCCATTCATAAGGTTTCTCACAGTGCGTTTTTCGATGC50.10264832683227264No Hit
CTGCTACGCAGCCATGTTTGGGCCTAAAGGCTTTGGGCGGGGCGGAGCCG50.10264832683227264No Hit
GGAGTCTACAGACTGAGTCATTAGCTGCTTGCAATCTCTGGAGAACCTGG50.10264832683227264No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA50.10264832683227264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.10264832683227264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)