FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.8846153846153846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGTATCACACGCGCTCTTCCGATCCTG32.8846153846153846No Hit
GATCGGAAGAGCGTCGTGTAGGGAGATGTGCTGTCTCTTATACACATCTC21.9230769230769231Illumina Single End PCR Primer 1 (96% over 26bp)
TCTCGGTGGTCGCCGATCACCCATGTACTCTGCGTTGATACCTGTCTCTT21.9230769230769231No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGCAGCGCGACACCGAGTTACACTCTTTCCCTACACGACGCTCT21.9230769230769231Illumina Single End PCR Primer 1 (96% over 27bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTT21.9230769230769231No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGATAGACGCCCGCATCCTGTAGTGCGGGAAACTGGGCGCGAAACTGCGC21.9230769230769231No Hit
GTGGGGCGAAGCGCTCGCGCAGCAGCCGTCCAGGCACCTGACTGTTCAGC21.9230769230769231No Hit
GCGCAAAGTCCACTGCGTCCTGCGCCATCTGGCGATACGACGTCAGCTTG21.9230769230769231No Hit
GCATATTTACGGTAGTGAGACACTCGAAGATGTAGAAGCAGCTCTAAAAC21.9230769230769231No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CGTATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGGTTTTTAAC10.9615384615384616No Hit
CAACGCAAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACGT10.9615384615384616No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGGGG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACC10.9615384615384616Clontech SMART CDS Primer II A (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.9615384615384616Clontech Universal Primer Mix Long (100% over 23bp)
GGCGTCGTGTAGGGAAAGGTGTAGACTCGGTGTCGCCGTACTTCCCATGT10.9615384615384616No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAGAGTGTAGATCTCGGTGCGCGTACCCATGTACTCTGCGTTGATA10.9615384615384616Illumina Single End PCR Primer 1 (95% over 23bp)
GTACATGGGACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGT10.9615384615384616Illumina Single End PCR Primer 1 (97% over 34bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGG10.9615384615384616No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTTCCCATGTCTGTCTCTTATA10.9615384615384616Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA10.9615384615384616Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGGAAAGAGTGTAGATCGGTGGTCGCCGATCATTCCCATGTACTCT10.9615384615384616No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.9615384615384616Illumina Single End PCR Primer 1 (100% over 37bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGCTTCCCTGTACTCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.9615384615384616No Hit
AGTGTAGACTGGTGGTCGCCGTCATTGCCCATGTACTCTGCGTTGATACC10.9615384615384616No Hit
GGCGGCGGAAATGCTGCGGCGCACCCGCGGCGGGGCAGCCGTGGTGATGG10.9615384615384616No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGATGCGTTTAAACCA10.9615384615384616No Hit
CAGTGTATCAACGCAGAGTACATGGGATCTACCCCTTAACAGTCTGTCTC10.9615384615384616No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATAAAGGTTTAT10.9615384615384616No Hit
CGGCGACTCATAGCGCAAAGGCGCCGGCCTTGCGAGTGGGAGGATCAGCC10.9615384615384616No Hit
GTACATGGGTGATACGGGACCACGAGATCTACACTCTTTCCCTGAGCGTC10.9615384615384616Illumina Single End PCR Primer 1 (95% over 22bp)
GTGCACGACGATGCCCGGTCCGCCACCGGCGACCCGGGCATCGTCGTGCA10.9615384615384616No Hit
CAACGCAAGAGTACTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACTG10.9615384615384616No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTTCAGGTATCGCGTA10.9615384615384616No Hit
GAGGTAGGTGGCCAGCGTCTCGAAGCGCAGCAGGGTATTGCCGGTTACGT10.9615384615384616No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CGCATAGTACATGGGATGGGCCACCGAGTCTACACGCTTTCCCAACACGA10.9615384615384616No Hit
GTATGACGAAGAAAAAAATGAAAGAAATCGTCATAAAAATGTAGAGGAAA10.9615384615384616No Hit
TCTTCATGCACTAAACCCGAGTTCGGCTCGGAACCTCGTGGGAGGGCGCT10.9615384615384616No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.9615384615384616Clontech Universal Primer Mix Long (96% over 25bp)
GCTCGCGCTGGGCGCCGAGGAGGACCCGGCGAAGTACAACATGGCCCACA10.9615384615384616No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATTGTGTGG10.9615384615384616Clontech Universal Primer Mix Long (95% over 21bp)
CTGCGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9615384615384616Clontech Universal Primer Mix Long (95% over 21bp)
CAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACAC10.9615384615384616No Hit
GCCCAGACGATCACCGAGTACAGGCCGAACCACCCGGCCGCGCCCAACGC10.9615384615384616No Hit
GTGATTTACTATAACTTGTTTAGTTGGTTGTTTTAAGCTTGGATGCATGA10.9615384615384616No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCT10.9615384615384616No Hit
ACCAGCGACACCTCGGTGCCCGAGCGGGTCAGCGCGGCGTGCGCCGAGGA10.9615384615384616No Hit
AAGCTGACACGACGCCGCCCATGGACGAGCCAGGCGCAGACGCAGCACAA10.9615384615384616No Hit
GTCATAGACCGAGTGCAGCATCGCTTTCACCGCCGGCCACATCGACTTCG10.9615384615384616No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GGAAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACAT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GAAAGGAAGGGCACGGTCAGCGAGCCGCCGCCGATGCCGAAGATCGCCGA10.9615384615384616No Hit
GTTTCAGGTGGAGCTAGGTGTCAAAGTCGCCAAGGCTTCGGATGAAATGC10.9615384615384616No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CGGCGTGGGCGGTGCCGGCCCCGCGGTGACGGCCCTCGGCGAGGACCGTC10.9615384615384616No Hit
GTACATGGGTGACGGCGCCACCGAGATCAACACACATTCCCAAGAAGAAG10.9615384615384616No Hit
CACTCTGTCTGTAAAGTCTGCAAGCAGATATTTGGACCTCTTTGGGGCCT10.9615384615384616No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGTGATCTCGTCGCGTCTCCCATGTACTCTGCGTTGATACCACTGC10.9615384615384616Clontech SMART CDS Primer II A (95% over 24bp)
GTACATGGGGTATCGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9615384615384616No Hit
NAGTGTAGATGGGTGCGTTCATCCCATGTACTCTGAGTTGATACCCCATG10.9615384615384616No Hit
GGCGAAGAGCCCTTGGTCTTGGTCAACATGAACGTTGTCTCCTGTCGTGG10.9615384615384616No Hit
ACGCTCTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACCAAAAAAGGGA10.9615384615384616No Hit
GCTTCCCATGTACTCTGCGTTGGCAGTGGTATCAACGCAGAGTACATGGG10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AATCGGAAGAGCGTCGTGTAGGGAAAGGTGAGACTCGTGGTGCCGATCAC10.9615384615384616Illumina Single End PCR Primer 1 (96% over 27bp)
CAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCA10.9615384615384616No Hit
AGATACACTCTTCTACACGGAGCTGCCGATACCAGTAAACATGAGATCGG10.9615384615384616No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CGACAAGGGCAGCCTCACCGTGACCGGCACCATCGACGCCAGCGGCGAGC10.9615384615384616No Hit
GCATCGGCGTGAACGCCGGCTCCCTCGACAAGCGCCTGCTCGCCAAGTAC10.9615384615384616No Hit
GTATTAGCCCCAATTTCTTGGAGTTATCCCAGAGCTGAGGGCAGGTTGCC10.9615384615384616No Hit
GTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAG10.9615384615384616No Hit
GCCCAGACGATCACCGAGTACAGGCAGAACCAGCCGGCCGCCCGCCCCGC10.9615384615384616No Hit
CATCCCTGCTTTAGTCCAGGTGCTTTTTTCACTCAACATTAAGTTTGTAA10.9615384615384616No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.9615384615384616Clontech SMART CDS Primer II A (100% over 26bp)
GGCCTGCGCAGCGAAGGGCAACAGACCGGTGAAGTTCAAGGGTGGCCGCC10.9615384615384616No Hit
GCTCAGTCCGGGATCAGGGCCCAGCCACGAAGCTGCATCGTCGCCATCGT10.9615384615384616No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTAT10.9615384615384616Clontech SMART CDS Primer II A (96% over 25bp)
CCGATACACTCTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAG10.9615384615384616Illumina Single End PCR Primer 1 (96% over 27bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGTGGTAT10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGTGAAGGCTCAAGTCCGGCCCATCAGTCCCGCCGGAGCCGACCGAT10.9615384615384616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)