FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003665998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003665998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54.807692307692308Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGCGTGTGATACTGCCCATGTACTCTGCGTTGATACCTG32.8846153846153846No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT32.8846153846153846Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.8846153846153846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACTATTCAGCATATGTTACTGACGCGGCTTCGCCAGGATATCCAGATA21.9230769230769231No Hit
GTATCAACGCAGAGTACATGGGTGATCGGCGACCACCGAGACTGTCTCTT21.9230769230769231No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGT21.9230769230769231Clontech Universal Primer Mix Long (95% over 21bp)
CACATCTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATACACATCTG21.9230769230769231Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGAGCGTCGTGTAG21.9230769230769231No Hit
TTCCATTACATATCCTCCTTTACTAATGACAATAGGGCAAGTTTGTAACC21.9230769230769231No Hit
GCCCGCGACGATCCAGCCGAACACCACCTGCCAGCCGACGGCCGCGATGG21.9230769230769231No Hit
GCCTCGAACCTGTTGCCGACAGGGAGGATATCGACTACCTGCTTGAACAT21.9230769230769231No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC21.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCGGGCGGTGCTCGACACGCTCCGCCAGCCGCTGGAGCGCGGGGAGG21.9230769230769231No Hit
AGCTCCGTCGTGTAGGGAAGAGTGGACGGGTCGCGACCCATGTACTCTGC21.9230769230769231No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT21.9230769230769231Clontech Universal Primer Mix Long (95% over 21bp)
TGGGAATGTCGGCGCCCCCCCACAACAAAACACATTACCGAAACCGCGCC10.9615384615384616No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAGCCTGGCAGCGGTGCCGGACCCACTACGCGGCGAACTTGATGGGC10.9615384615384616No Hit
CCGCCATAAGCAGTGGGATCACCGGTTGGTTATCGGACGGCACCTCGCAG10.9615384615384616No Hit
GTACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
ATCAGGCCCAGGCCTATCATGCCGATCTGCTTCATATTGTCTCCTCTTGT10.9615384615384616No Hit
ACTGTTAAGGGGTAGATCCCATGTACTCTGCGTTGATACACTGCTGTCTC10.9615384615384616No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTTCCCTGTCTCTTATACACAT10.9615384615384616Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.9615384615384616Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAATGACGGCGACCACCAGTCTACACTC10.9615384615384616No Hit
CCCATCACCACGGCTGCCCCGCCGCGGGTGCGCCGCAGCATTTCCGCCGC10.9615384615384616No Hit
ATGCAGGCCCGCTCAACAGAAAAAGTGCGGGCTAAGCGTAGGTGTCGATC10.9615384615384616No Hit
NTACATGGGGTATCAACTCAGAGTACATGGGATGAACGCACCCATCTACA10.9615384615384616No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.9615384615384616No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAAAACACATC10.9615384615384616No Hit
TAACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGTTAC10.9615384615384616Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GAGCACTCCCACAGCGCCGACTCGACCAGCGCCTCGGGGGTGGCCTTGCC10.9615384615384616No Hit
CATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAAC10.9615384615384616Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9615384615384616No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCACTGTCTCTTAT10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAATGTAATC10.9615384615384616No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9615384615384616No Hit
GGCCCATGTGGGCCATGTTGTACTTCGCCGGGTCCTCCTCGGCGCCCAGC10.9615384615384616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGCGCACCG10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCGAAAGTCAGTGCCAAGTAATACCCCGTGCCGGGCTCCTTGTGGGTT10.9615384615384616No Hit
TATCAACGCAGAGTACATGGGAAGTACGGCGACACCGAGTCTACACCTTT10.9615384615384616No Hit
TCCTCTCCCTCCGGGATCGCGAGCAGCGGACGGAGGAGAGGCCATGGACC10.9615384615384616No Hit
GCGTTGATACCAGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9615384615384616No Hit
GTATCAACGCAGAGTACATGGGAACGCCGATACACTCTTCCCTACACGAC10.9615384615384616No Hit
GATGAACGATGCTGCTTATAGCGAGCGGACCAAGGTTGCCAGAAACCCTA10.9615384615384616No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGAGTACAGGGAAGCAG10.9615384615384616Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA10.9615384615384616No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTGGGTTAAACCACATGGTACCGGTGG10.9615384615384616No Hit
ACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA10.9615384615384616Clontech Universal Primer Mix Long (96% over 27bp)
CTGCAGAAGATCATCGGCAGCTTCGCCGTGCTGGTATCGATCCAGATGGC10.9615384615384616No Hit
CAACGCAGAGTACATGGGAATGATCGGCGACCACCGATCTACACTCTTTC10.9615384615384616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCAT10.9615384615384616Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGTGATAGCGCCCGAGATACACTCT10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ACTCTGCGTTGATACCACTCTTCCCATGTACTCTGCGTTGATACCACTGC10.9615384615384616Clontech SMART CDS Primer II A (95% over 24bp)
TTTCTACATTTGGCCTAAAAGCGCTTGAAATCTCCACCTGCAAATATCAC10.9615384615384616No Hit
GAGTACATGGGCCACGGTACACTCTTCCTACCGACGCTTGCCTCAGATTC10.9615384615384616No Hit
GCTGATCCTCCCACTCGCAAGGCCGGCGCCTTTGCGCTATGAGTCGCCGC10.9615384615384616No Hit
GTCTTCGACACCGCCGGCTGGCTCAGCGCCAGCCGCTCGGCCGCGCGCGC10.9615384615384616No Hit
GTTCTGGCCGGGCTCGCCGCCCGCCTTGGCGTTGGTGCCGTGGCGCAGGT10.9615384615384616No Hit
CAGACCAGGAGCCCAGGCACCAGTAACCACGTCCCCGCACGGTGGGAGGT10.9615384615384616No Hit
GTACTAACTATTATTCGGCCAAATTTGTCCTGCAATTTGTCCGTTATAAT10.9615384615384616No Hit
GAGGGGGAGGGGGTGAATCAGTTACTCAAGGTCTACAATTACTAATTCAA10.9615384615384616No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT10.9615384615384616Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGGTGCTTGCCTTGTTAAATCAAACCCTCAGCTCATAAGGAAGTGG10.9615384615384616No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.9615384615384616Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAATACTTT10.9615384615384616Clontech SMART CDS Primer II A (96% over 26bp)
GATAGGGAACGAGTCTACACCTTCCCTACACGACGCTCTTCCGATCTATC10.9615384615384616Illumina Single End PCR Primer 1 (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAATGATACGCC10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCACCAGTTCGAGCGCCAGGAAGATCGCCCCCAGGGTGAGGAAACTGG10.9615384615384616No Hit
CCGCCACCACGGCCAGCGCCAGCAAGAAGTGGCGCAAGGGCAGGGCGGCG10.9615384615384616No Hit
GTGATGCGCCGCCGGTGCTGCGCTTCGACGACGGCCTCGTCGTCCCGGTG10.9615384615384616No Hit
TTCGTGCAGTCGGTGCGGTGCACGCTCACGCCGCCGCCGCGGGTGACGAA10.9615384615384616No Hit
CACCTGTAGTCCCATCTACTTGGGAGGCTGAGGCAGGAGAAATCACTTGA10.9615384615384616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGCAGCTG10.9615384615384616Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)