FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA464.563492063492063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT373.670634920634921Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG131.2896825396825395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC131.2896825396825395Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA90.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG90.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT80.7936507936507936Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60.5952380952380952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60.5952380952380952Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT60.5952380952380952Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG60.5952380952380952Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC60.5952380952380952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50.496031746031746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG50.496031746031746Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.496031746031746Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG50.496031746031746Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA50.496031746031746Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT40.3968253968253968No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.3968253968253968Clontech SMART CDS Primer II A (100% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT40.3968253968253968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.2976190476190476Clontech Universal Primer Mix Long (100% over 23bp)
ATGACTAAAGGTCTATTTATCATAGTTCCCTTTACACTGTACATCAAGTC30.2976190476190476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.2976190476190476Clontech Universal Primer Mix Long (95% over 21bp)
TGCTGAGGGAGATAGACATGAAGTACATGTAAATGGGTAGACTAGAAGTG30.2976190476190476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.2976190476190476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG30.2976190476190476Clontech SMART CDS Primer II A (96% over 26bp)
GGCTCAGTGCAACCTCTGCCTCCTGGGTTCAAGTGATTCTCATGCCTCAG30.2976190476190476No Hit
GTACATGGGAAAACGAGTGCTGATCTCCACGGTGGAACTGCAAAGTGGTA30.2976190476190476No Hit
GTCCTCTCTCCAAACAAACAAACAAAAACCCAATGTAGCTACAATGTCAG30.2976190476190476No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA30.2976190476190476No Hit
CACACGTGCCTGCCCGGCTATCGTAGCCAGGCTGGCAGCCTTGCAACTCC30.2976190476190476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2976190476190476No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.2976190476190476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGACTGTCT30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
CGCGAGGGCTGACCGGACGGGGCGCCTCCAGCTCTATGACACACTGTAGC20.1984126984126984No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20.1984126984126984No Hit
GGTATCAACGCAGAGTACATGGGAAGCTGTGGTATCAACGCAAAAAAAAA20.1984126984126984No Hit
GGCACAGACTGGGGTATGGGCACAGGTGAGCATCTCCATATGAGCCGTAC20.1984126984126984No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT20.1984126984126984No Hit
GTTAATGAATCTGGACCCCTAGAATGAAAATGAGATGAGAAACCGAAATC20.1984126984126984No Hit
GTTCCAGCAGCTCAGGCTCCTTCCCATTGGTTCTCACAAAGTGTGCTTCT20.1984126984126984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.1984126984126984Clontech SMART CDS Primer II A (100% over 26bp)
CCATAAGAAAGCTGGTGTAGCAGTATCGATGACCTGAAATGGGATTCAGG20.1984126984126984No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTGAGCGACTCCAGCCTGCGCGAACAGATCAAGTCCAGCACCGACGC20.1984126984126984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCAGGCTCCGTGGCCCCCACCGCTGTTCGCGCACCCGGTCTGCGGCTTC20.1984126984126984No Hit
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACAT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GGCAACAGAGTGAGACTCTGTTTAAAATAATAAAATAAAAAAAACCCAAA20.1984126984126984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTTCATGGGACCGAGATTCT20.1984126984126984Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA20.1984126984126984Clontech Universal Primer Mix Long (95% over 22bp)
CACCGACTCTGCGAGACCCGTGTATCATCCCGGTGAGGAAGAGATAATTC20.1984126984126984No Hit
GTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCTG20.1984126984126984Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
ACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTA20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGATCGGCCCGATTACACTCTTTCCCTACACGACGCTCTTCTGT20.1984126984126984Illumina Single End PCR Primer 1 (96% over 30bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.1984126984126984Clontech Universal Primer Mix Long (96% over 25bp)
GATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGCA20.1984126984126984No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CTTCAACGTCAACACCGGCGTGGTCGACAAGCGCTGGTTCCAGGGGCTGC20.1984126984126984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT20.1984126984126984No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CCATTATACAAAAAGTAAAAACATTGCTCATGGTCATTACAAAATACGGG20.1984126984126984No Hit
TCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGG20.1984126984126984No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTCTGT20.1984126984126984Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTGTAGATCTCGGGGTCGCCATCCCATGTACTCTGCGTTGATACCAC20.1984126984126984Clontech SMART CDS Primer II A (95% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCATCACGCGGGTCGTAGAGCTTGGCACCACGAATGCGGGCCGTCAC20.1984126984126984No Hit
CACTGCTCCCATAGGGAACAGAGATCCTGGCTGAGCAGTCGCGGGCTTGG20.1984126984126984No Hit
GTACCGGCCGTGCGTACTTACACATGCATGGCTTAATCTTTGAGACAAGC20.1984126984126984No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.1984126984126984Clontech Universal Primer Mix Long (96% over 25bp)
GTCGTTCGATGGCGCGACGGAAACACGCTTCGGCTTCGGCGAACTCACCG20.1984126984126984No Hit
GAGATCTATTGTACAGCATGGACCATAGATCTCAAAATGCAAAACAATAG20.1984126984126984No Hit
AAATAACATGATATCTATTTGGTGTTTCAACACTTCTTGGTGGTAATTCA20.1984126984126984No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCCAGGTCTTGTCGATACAAG20.1984126984126984Illumina Single End PCR Primer 1 (96% over 33bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1984126984126984No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCA20.1984126984126984Clontech Universal Primer Mix Long (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA20.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
GGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCC20.1984126984126984No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGC20.1984126984126984No Hit
CCATTCGTAGAGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAC20.1984126984126984No Hit
TCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCACGTTTACCCCCAG20.1984126984126984No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1984126984126984Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGAGTGTAGCTCGGCCACGAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1984126984126984No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTATAC20.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGTGAAACCCCGTTTCTACTAAAAATACAAAAATTAGCTGGGCGTG20.1984126984126984No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG20.1984126984126984No Hit
TTCTTCAGTTTGTCAACTGAATTTTTCAGCCCCAGAATTTCTGCTTGATT20.1984126984126984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.1984126984126984No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.1984126984126984Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1984126984126984No Hit
GCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCT20.1984126984126984No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGTTCCCATGTACTCTGCG20.1984126984126984Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGCCTGGAGCCTCATTCATCATGGCATCTAGATACTCATCAGTTGGATT20.1984126984126984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)