FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA525.158730158730158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT262.579365079365079Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG161.5873015873015872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG161.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151.488095238095238Clontech SMART CDS Primer II A (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA100.992063492063492Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG100.992063492063492Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC90.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC70.6944444444444444Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.5952380952380952Clontech Universal Primer Mix Long (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC60.5952380952380952Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT60.5952380952380952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60.5952380952380952Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA50.496031746031746Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.496031746031746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.3968253968253968Clontech Universal Primer Mix Long (95% over 21bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40.3968253968253968Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40.3968253968253968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG40.3968253968253968Clontech SMART CDS Primer II A (96% over 26bp)
GCCTCAGCCTCCGGAGTAGCTGGGACTGCAGGCATGAGCCACTACGTCCG40.3968253968253968No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT40.3968253968253968Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCT40.3968253968253968No Hit
TTTATGGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACAAC30.2976190476190476No Hit
ACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTG30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
GCTACTTGGGAAGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGGGGAG30.2976190476190476No Hit
ATGTATTCTTTAATGTAAGTTATACACAATAAAACATACCTATACAAATG30.2976190476190476No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT30.2976190476190476Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.2976190476190476Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT30.2976190476190476Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGTGGTGAAACCCCGTCTCTACTACAAATACAAAAATTAGCCG30.2976190476190476No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.2976190476190476Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT30.2976190476190476Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2976190476190476No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.2976190476190476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGCCTGGCCAAAGATGGCACTCAAAAAGCTGTGGCCACTGTTAGTGCTA30.2976190476190476No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA30.2976190476190476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2976190476190476Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA20.1984126984126984Illumina Single End PCR Primer 1 (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG20.1984126984126984Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTGCGGACCACCAGATCTCACTCTGTCGTGTAGGAAAGAGTG20.1984126984126984No Hit
TGTTTGCGCTGCCGGATACGATCGTTGCCTATATGGTCGCCATCGGCGCG20.1984126984126984No Hit
GTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCCATTTCAGCTCAC20.1984126984126984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCTG20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.1984126984126984Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGATGAACGGCGACCACCGAGATCTACACTC20.1984126984126984Illumina Single End PCR Primer 1 (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTAT20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.1984126984126984Clontech SMART CDS Primer II A (100% over 26bp)
TCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA20.1984126984126984No Hit
CACCAGGGTCTTGGTACGAACCAGCTCGTTATTAGATGCATTGTAGACAA20.1984126984126984No Hit
TCAGGCGTCTTGGTAACTAAGCACTTTTCTGGGTGAGCATCTTATCACAG20.1984126984126984No Hit
GAGTACATGGGACCGAGATTCTTTTCCCTACACGACGCTCTTCCGATCTC20.1984126984126984Illumina Single End PCR Primer 1 (96% over 28bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATAC20.1984126984126984No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAA20.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
CACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCA20.1984126984126984No Hit
CTATGAACTGTCCTGAATCCCATTTCAGGTCATCGATACTGCTACACCAG20.1984126984126984No Hit
TGACCCTCGCCCCGCCCCGAAGCCGTGCCCTGGAATGTGCGGCCAGGCAA20.1984126984126984No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTGTCTCTTA20.1984126984126984Clontech Universal Primer Mix Long (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAATACCGCCACGCCACGCGCGGTCCCGTCCCCTACTGGCGCGAGGC20.1984126984126984No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG20.1984126984126984No Hit
GCGTCAGCTCACCGCGCGCGAGGCGTCGCGTATGACAGACGACATACGCG20.1984126984126984No Hit
GATTTGGTTTGACCAAGAATATTTCTAAACCAGAAACACAAGAGGAATGA20.1984126984126984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAA20.1984126984126984Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGAGCCCAATAAAGACTGTTAATTCCTCAAAAAAAAAAAAAAAAAA20.1984126984126984No Hit
CATGGGAAGCAGTGGTATCAACGCAGCTTACATGTACTCTGCGTTGATAC20.1984126984126984Clontech Universal Primer Mix Long (95% over 23bp)
GAGGGAGTCAGGAGTCAGGGCACTCCAGGGATCAAAGGCAAGGGTACAGG20.1984126984126984No Hit
CTACTCGACCGCGCCGTGGGGTGGCGTCGAGCAGCAGGATTTCTACAACG20.1984126984126984No Hit
CTTGTCACCTTTTGGGACCCATCATGTTCCATTTTTTTTAAACACTAAAA20.1984126984126984No Hit
GCTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTC20.1984126984126984No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTATACCACTGCTTCCCAT20.1984126984126984No Hit
GAATGGGGAGAAAAGAAAGCGTTTGAAATTATTGCTTGGATACCAGCTTC20.1984126984126984No Hit
ATATTGTGATTATCAAAGATAATTCTGGTCACACTGGCGTAGGGGAAATT20.1984126984126984No Hit
AAGCTGTAGAACGGCGCATTGGTGACCATCGTGAGGCCCGCGTACAGAGT20.1984126984126984No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA20.1984126984126984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA20.1984126984126984No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGA20.1984126984126984No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT20.1984126984126984No Hit
ACTTAAGTCATAGTTAAGATCTGTGATACGAACCATAGATATTGCCTGAC20.1984126984126984No Hit
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 25bp)
CCCCTGTACCCGGCTGTCCCCCATCTCCTGCCTATTACTCCCCGGCCACC20.1984126984126984No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.1984126984126984Clontech SMART CDS Primer II A (96% over 26bp)
GCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGC20.1984126984126984No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA20.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAGTACTTTTTTT20.1984126984126984Clontech Universal Primer Mix Long (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1984126984126984No Hit
AAGAGCGTCGTGTAGGGAAAGAGTGTAATCGGGCCGATCCATGTACCTGT20.1984126984126984Illumina Single End PCR Primer 1 (96% over 27bp)
CAGTGGTATCAACGCAAAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.1984126984126984Clontech Universal Primer Mix Long (100% over 23bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
AGTTAAGGGCAAGATTCTTGCCAAGAGAATTAATGTGCGTATTGAGCACA20.1984126984126984No Hit
GAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGA20.1984126984126984No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.1984126984126984Clontech Universal Primer Mix Long (96% over 26bp)
GCGCTGATGACGGTCGGCTTCATGCGTTCAGGAGCCGGCGCGGCGGCGCC20.1984126984126984No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.1984126984126984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAG20.1984126984126984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)