FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTTGCAGTGAGCCATGATCTCACCTCTGTGCTCCAGCCTGGGTGACAG34.411764705882353No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34.411764705882353Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.411764705882353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGTGCATCGACCTGCTGGTTTGCAAGCTGCACGATCGATAGCTCCCGG22.941176470588235No Hit
CCCATGAACGGCGTGCTGGGCCTGGTCGAACTGCTGCAGCAGACCAAGCT22.941176470588235No Hit
GTGTGCATTTATCTGGATGCGCCAGACGTGCATTCGAGTGCGGGCGGCAG22.941176470588235No Hit
ACGCCATGCCTTTGAGCCGCGCCCACAGCTGCCGCAAGCGCCGCAACCTC22.941176470588235No Hit
GATGTAGATCTCGGTGGTCGCCGCATCCCATGTACTCTGCGTTGATACTG22.941176470588235Illumina Single End PCR Primer 1 (96% over 25bp)
CCGGCACCACTCCTCCTCGCATGGCCGATGTTGCGCGCGTTGCCGGCGTG22.941176470588235No Hit
ACGTGGGACTTGTTTCTCGAAGAAGATTTCCGCTGCCGCCTGCCCGGCAT22.941176470588235No Hit
GCGTCGTACTCTGGCAGCAGGCTTTCCATGCAGTTGAAGACGGCCTCGTA22.941176470588235No Hit
GTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAGGG22.941176470588235Illumina Single End PCR Primer 1 (100% over 24bp)
GCCGCACGCAGTGCATCGGCGTCGCCGAACGGAATGCGCCGGAAGCCCGC11.4705882352941175No Hit
TCAGATCAATGATGGTCATGTCACTCCCCTTGCGAGTTCTTCCTCACGCC11.4705882352941175No Hit
CAATACGCCCGTGCCGAGCATCACCACGCCTGCCGCCAGCACCGCCGATA11.4705882352941175No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTG11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
ATCTGAGGCAAGAGCGTCGTGTAGAAAGTAGATCGCCCCATGTACTCTGC11.4705882352941175No Hit
CCTGTGCCGGCGTTCTTTCGCTGGCTGCATCGCAATATGCAACCGCCCAG11.4705882352941175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.4705882352941175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
TGGCCGCCACGTCCATCACGAGCGTGTCGCCGGGGCGGATGTCAAAGCGG11.4705882352941175No Hit
GGTGAAAATTCTCTAATCATGGAAGACATCTTTTTCAAGGCAGAGAATTT11.4705882352941175No Hit
GGTAGGTGTGGTCAAGATTACAGGGCTGCCCTTCTCCCCACCTCCCAGCC11.4705882352941175No Hit
TGGGGTGTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACCT11.4705882352941175Illumina Single End PCR Primer 1 (97% over 37bp)
GAGTGGGGGAGTCTCACGGGGCCCCAGGCTTATTCAGAACTGGTGTTTTT11.4705882352941175No Hit
CGCGGTATTCCTCGATCTGCTCTTCGCCGAATGCGCCGATGATGGCGCCG11.4705882352941175No Hit
GCATTGAAGCGCTGTGACAGTGCCACCGCGCGCTGCCATTGCGCGCGCTG11.4705882352941175No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC11.4705882352941175No Hit
GATGGCCGGCCACGGCCCCGATGTCGCCAGCTGGCAGGCGGCGAGCGCGG11.4705882352941175No Hit
GATACACTCTTTCCCTACACGACGCTATCTGAGGCAAGCGTCGTGTAGGG11.4705882352941175Illumina Single End PCR Primer 1 (96% over 26bp)
GATCCAAATTGCGTTTGAACTAGGAGACGCACAAATGAAATTGAAGCAAC11.4705882352941175No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA11.4705882352941175No Hit
GCCTTGGCCTGTTCGACCTGGCTGCGATCCAGCGCATCGGCTGCCGCAGA11.4705882352941175No Hit
TATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTCCGAGC11.4705882352941175No Hit
CGCGCGGCACCGAACGGGTGATGGCCGGCGCACAGGCCGACGCGGTGATC11.4705882352941175No Hit
CGCGTGCGTTGCTGGAGCGCGCCGGCATCCCCATCGTCGAGATGTGGGAT11.4705882352941175No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGT11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
CTTTCTCGCATTATAGACCAGTAGCTCTTTGAGAAGTGAGAACATTCCTT11.4705882352941175No Hit
CGTCAGTTCATTGCCGGGGCCATACAGCTCGCGCACTACCTGCGCGGTCC11.4705882352941175No Hit
GCGATATCACGCGTTTTCTGCTCGACACGATCTTCTCGCTCTTCGGCGCG11.4705882352941175No Hit
CGCTCATGCCTTGGACCAGCCGCGTCGGGTTGACGACCGCCGGCGAGAGC11.4705882352941175No Hit
GACGACACAGAATATCGCCGCGCTGTACAACCACGGCGACGCCGCGTACA11.4705882352941175No Hit
CTCATGGGCTCAAATGATCCTCCTGCTTCAGCCTTCCAAAACCAGGTGTT11.4705882352941175No Hit
GATCCGTTCATCTTCTGCTTCTGGTCTTCATCATCCTGACGGTGTTCCTC11.4705882352941175No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGGGTCCTGATCTCGTA11.4705882352941175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT11.4705882352941175No Hit
TACGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC11.4705882352941175No Hit
GCTTCATAGAGGGGATTCAGGTGGGGGTGCCCTCGGTCTGTCTCTTATAC11.4705882352941175No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATTATGGGTTTA11.4705882352941175No Hit
CAGCAGCATCGCGATGATCGGGCGGCGGCTCGCATGCAGCTGCGGCAGGG11.4705882352941175No Hit
GTATCAACGCAGAGTACATGGGAAGCGCAGTGGTATCAACGCAAAAAAAA11.4705882352941175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)