FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA34.411764705882353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCATACGCCACCATGCCTGGCTAATTTTTGTATTTTTGTAGAGGCAGGG34.411764705882353No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC22.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
CCGTTCGGGGCGGGCGTTCGCGTTGCGGTCGAGCAAGGCTCTTCACTGGA22.941176470588235No Hit
CGGGGCTTCCATGTGCGGTTCCGGCCGGCGCTGCTCCGGCTCCAGCCGGG22.941176470588235No Hit
CACAATCAACTGTCCCGACAGCGTCAACGGCAACTTGCGGCTGGCCGCAT22.941176470588235No Hit
AGTTGGAACTGGCCAGCGAAGGCACCAGCGCGGCGACGAATTCCGATCGG22.941176470588235No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
AATGATGCCGATGGGCTTGGGCAAGCCGCGCAGCCATTCGATCAACCGGC22.941176470588235No Hit
TCCACGTGCATGGCGAGCGCTTTTTCGTGCGCGCGGCTCGCCAGCAGGCC22.941176470588235No Hit
TATCAACGCAGAGTACATGGGATGCGGCGACCACCGAGATCTACATCCTG22.941176470588235Illumina Single End PCR Primer 1 (95% over 21bp)
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT22.941176470588235No Hit
CTCCTTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGACAAGACC22.941176470588235Illumina Single End PCR Primer 1 (96% over 30bp)
CAAGTTCGCGGCCGAGGCCGACGTGCTGATCGAGAACTTCCGCCCCGGCG22.941176470588235No Hit
GTTCCACCTCGGCAAGCTTTTCTGCCAGCCCCATCGAGAACACCGACACC11.4705882352941175No Hit
CACCGGCCGCCCGGCGGGTGTGCTGGCCATCGTGGTGGAAACCACCGAGC11.4705882352941175No Hit
GTTAGAAATGTACATGTTGCCAAAATAATAATAATAAAATTAAAAAAAAG11.4705882352941175No Hit
CTGAAAACGCTGGTCGGCCACGGCGTTCGGCAGGTGCGTGGACGCGGCTT11.4705882352941175No Hit
TCCCTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTG11.4705882352941175Illumina Single End PCR Primer 1 (100% over 25bp)
GATATAGCCCGTGGCCGGAATCACCCGGCGCAGCGGGTGCACCAGCCAGC11.4705882352941175No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.4705882352941175Clontech Universal Primer Mix Long (100% over 23bp)
ATGAAGGCCATTGTGCCGTCGATCTTGTGCGGCTTCAGCTCCGCCGCGCT11.4705882352941175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG11.4705882352941175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGC11.4705882352941175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGATGGGCGATTCGCCCAGGCGGATCACACGCGGCTCGCCCAGGCCAAG11.4705882352941175No Hit
GGCGCACGGCAGCCGGCGTGTTGTCAGCAGCGGCGCGTGTGCGAGCCATG11.4705882352941175No Hit
GAGGAGAACCCGACGATGGTCGTCGTGGGGGGATAAAATGATTGAGTGGG11.4705882352941175No Hit
GTTCGATTACGGCCAGAGCGGACTGGCCCGCCAGGCGCTGCCGGCCGATC11.4705882352941175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACTG11.4705882352941175No Hit
GACCAGTTGCCGCGCACCCATTCCGACACGTTTGCGCTCACGCACAAGCT11.4705882352941175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.4705882352941175No Hit
GTATCAACGCAGAGTACATGGGATATAGGCCACAGATACACTCTTTCCCT11.4705882352941175No Hit
GCGGTCCAGCAGGGCGGAGGCGCGCGAGCCGCTTTCGCCCGCGTCGCCGG11.4705882352941175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.4705882352941175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAGTACTT11.4705882352941175Clontech Universal Primer Mix Long (95% over 21bp)
CGGCAAGACCGCAGCGCTTGCGGCGGTCTTCAATCTTGAACGCCGCGTGC11.4705882352941175No Hit
GCCTCTCAACTGTACTTGAGTAGAATTTTATATCTGCAATACATAGCTGG11.4705882352941175No Hit
GAGACGGTGTAGCGGTGCGTTGATGATGACGCATCGCCACACCGTTTTTT11.4705882352941175No Hit
CCCATGAAAAGGCTGTCCCACTCCATCGACGTGGCGAAGCAGTTTCTCGA11.4705882352941175No Hit
CTCTTCAACGGCCAGGGTCGCGCCGTTCTTCATGTCCGTGCCGTATTGCG11.4705882352941175No Hit
GCGCGTGCAGGTCGAGACCGACTACAACCACGGCACCAAGAGCCGCAAGA11.4705882352941175No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT11.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
CTACCGCACGAAGTCTGACGACGCCTGGAAGCAATACAAGACCCTGCCCA11.4705882352941175No Hit
ATGTCCCCCATTTGCTGCAACTCTCAGTGGTAAGAATGATTCAGTGCAGC11.4705882352941175No Hit
ACCGAGGGCACCCCCACCTGAATCCCCTCTATGAAGCCTGTCTCTTATAC11.4705882352941175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAACA11.4705882352941175No Hit
GCGCCGGTGCGGGTCAGTTGGCGCGGCAGGTCGTAGTTCTTGCCCTTCCA11.4705882352941175No Hit
GCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCT11.4705882352941175No Hit
CTGCACCAGCACAGCAATATCGCCCACAGCAGGCTCCGCTGAGGCCAAGC11.4705882352941175No Hit
GTGCAGGCTAATTATATAAGCCAGCTGAAAATTGCGTGTGTTTAATGGAA11.4705882352941175No Hit
GTACTTTTTTTTTTTTTTTGCGTTGATACCACTGCGCTTCCCATGTACTC11.4705882352941175No Hit
GTACATGGGTGTACGGCGACCACCCAGATCTACACACATTACCAACACCA11.4705882352941175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)