FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCACATTGAGTCCTTCCAAATGGATACATAGACCACACCCCAAAAAT42.5974025974025974No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.948051948051948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTACACTTGACTTGATTTTCTAGTTTTCTATACAAACACCAATGGGTTC31.948051948051948No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGGTATCAAC21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTGTGCAGAATGGCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCC21.2987012987012987No Hit
CCGCAGAAGCAGCACCTTCAGACGGCGACGCGGAACTCGATACGGTGCAG21.2987012987012987No Hit
TATCAAGTATGCCTACGTGGCCTGTGAGATCCTGTCCTCCGATGTCTGGT21.2987012987012987No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TATACTTACTTTGGACAAGCATTTTTTGCTGAAAGCTTACCCAAGCTTCG21.2987012987012987No Hit
TTCCCTACACGGAGCTGCCGATACCAGTAACCATGAGATCGGCAGCTCCG21.2987012987012987No Hit
ACCCTGTGCAAGCCCCTGGTCCCAAGGGAACATCAGTTCTACGAGACCCT21.2987012987012987No Hit
GTCTTATAGTGTGTTGTGAGAATCACATGTAATAAACCAGAAAGGTGATA21.2987012987012987No Hit
TCTCTGTAGTAATGACTTTTCTTCTTGTATTTTCCCGGATTATCCGCCCG21.2987012987012987No Hit
GTATCAACGCAGAGTACATGGGTGACCGACCACCGAGATTACACTCTTCC21.2987012987012987No Hit
GTACATGGGAAGGCCTTCCCTACACAGCAACCTGTCTCTTATACACATCT21.2987012987012987No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGT21.2987012987012987Clontech Universal Primer Mix Long (100% over 23bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAA21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCAGGACTTCTGTTATGGGTTAAATTGTGTTTCCCCCAAATTCATAT21.2987012987012987No Hit
ATGTTGGCCAGGCTGGTCTCGAACACCTGACCTCAAGTTACCCGCCCGCC21.2987012987012987No Hit
GGCGGAGTCACGCCTGGAACGCGGGGCTACCAGGCAGCAGTATCCGATTC10.6493506493506493No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAACGAGAGTCTACACTCTTCCT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT10.6493506493506493No Hit
GTGTAGATCTGGTGGCGCGCAGGATGAGTCCGGTATGCCCTGAACTCTGC10.6493506493506493No Hit
GAACCAAGCAGTGGTTCTCAATGCAGGGTGGCCTCAGAAATCCCTGCTCA10.6493506493506493No Hit
GCTGCCTCTCCCACAATCATTACACCTCTCTCTCTGATAATGTAACTACA10.6493506493506493No Hit
GGAACAAGACTCCCTCTCAAAAAAAAAAATAAATAAAAAAACACAGGCAT10.6493506493506493No Hit
CGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATCCCATGTAC10.6493506493506493Illumina Single End PCR Primer 1 (97% over 38bp)
AAAGTGTGCTGGTGGCATAGCAGTTGAGGCTTTGATGGGAGACCCCGCCT10.6493506493506493No Hit
ACGCTCTTCCGATCTCATGATGCAACTCCAGTCCTTGCATCATGAGATCG10.6493506493506493No Hit
ATGTTCTTCAAGGTCTTATGGCTTTTTTATGTTATTTTTCCACCTTGTGC10.6493506493506493No Hit
GTTCCTGGTGGAGCACGTGGTGGTCACGCAGCTGGAGGACGCCAACCACC10.6493506493506493No Hit
CAGAAAGGTTCCAAAGCGAGCAGCCACCGCGCTTCTCGTTCTGCGAGAGT10.6493506493506493No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTACTCTGCGTTGATACCAATG10.6493506493506493Clontech SMART CDS Primer II A (95% over 21bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CTTGAACATGGCTCCCGCATGTCTCCATCAGATACAGTAGTCGCTCTTCT10.6493506493506493No Hit
GTGTCATCGAACAGTAAGTCTGCTGTGAATAAGTTTATCGTACTTGTAGA10.6493506493506493No Hit
GCCTCGAACTCCTGAGCTCAGGCAATTCACTTGCCTTGGCCTCCCAAACT10.6493506493506493No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGCTGCAGGAGCTGCGGATGAACACACAAGGGCAAAGTGGAGACGGT10.6493506493506493No Hit
CATTCGATGTGGCGCGGCGCGTGAAGAGGAGTCGTGTCGTGAAAAATTCC10.6493506493506493No Hit
GAGTACAGGTCTCATCACACAAGGGGTTTGTTGAAAGAGTAGGTTGGTCC10.6493506493506493No Hit
GACTGAAGTCGGACTGGGCCTCCGCACGCCTGGCCGCCGCCTGAGCCCCG10.6493506493506493No Hit
AAATAGTAGGGCGTGGTGTTATGTGCCTGTAGTCCCAGCTACTTAGGAGG10.6493506493506493No Hit
AGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATT10.6493506493506493No Hit
GCATAGCGCGGCAGGCCGCCGGGCGGCAGCGGGTTGCCACCGAACTCGCC10.6493506493506493No Hit
TGTCAACGAGGTCAAGCAATCACGTAGAACCGAGTTTGAGAAGCGGAGCA10.6493506493506493No Hit
CAACGCAGAGTACATGGGTAGCGACCCGAGATCTAACTCTTTCCTACACG10.6493506493506493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TTTAAGCTCTTTGCCAACTACTTTCATGATCATTATCATTTCCACATCAT10.6493506493506493No Hit
CGATCAGGCAGGCAAACTCGAACTCGCCTGCGCGGTTGAAGGTCAAGACG10.6493506493506493No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGACTGTCTCTTATACACATCTC10.6493506493506493No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAG10.6493506493506493Illumina Single End PCR Primer 1 (100% over 25bp)
GTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTATATTCAGGAATGTATTTTTGTTAGGTCTCTGTGCACCCCTCTAC10.6493506493506493No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACATACCTCGGCATGAAATTTCTGGGTCATAGGATAACTGCATTTAAC10.6493506493506493No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCAC10.6493506493506493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAATT10.6493506493506493Clontech Universal Primer Mix Long (95% over 21bp)
CTACATGACAGAGCAGTTGGGAGCACTAAATAAAATACCTGTCTCTTATA10.6493506493506493No Hit
AATGATGAATGCTCGGTATGCGATCATCATCCCGAGCATTCATCATTCTC10.6493506493506493No Hit
CCTATATCCATATTGGTGAGTTTGAGTTAACACTGAATTTTTTTCTTCCT10.6493506493506493No Hit
GAGTGTATCTGTTCCCTCCCATGTACTCTGCGTTGATACTGTCTCTTATA10.6493506493506493No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGGTATCATTCCCATGTACCTG10.6493506493506493Illumina Single End PCR Primer 1 (96% over 30bp)
CACTATATCCACCATGATGTCCTCCAAAAATGAGGTTTTCTGTTTGCCAC10.6493506493506493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGGGGGGGGGGGCGGGGG10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCGCTTTATCGCGCGGGCCGCGCGGGCGTGAAGGTGGACTGCATCATC10.6493506493506493No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA10.6493506493506493Illumina Single End PCR Primer 1 (100% over 36bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GACCTATAACGCCGACCGTCTGGGCATCTTCACGCCGAAACAGGTTGACC10.6493506493506493No Hit
ACCGAGATCTACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCT10.6493506493506493Illumina Single End PCR Primer 1 (96% over 32bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGGGGTATCAA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGAAACCACTGCTTCCCATGAACTCTGCGTTGAAACCACTG10.6493506493506493Clontech Universal Primer Mix Long (95% over 23bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493Clontech Universal Primer Mix Long (95% over 21bp)
GAATTACCACCACCGCGAAGAGCGACGAGGAAGCCAAGGCCCTCCTGTCT10.6493506493506493No Hit
GGCCCTGCACGTTGGAGTGGCCCCGAACCGGACAGATTCCGGTGCCCGCC10.6493506493506493No Hit
GGTCCACTGCAGGTGAGAACACCTTTCAGGTGCTGGAGTTTAACCTGGCT10.6493506493506493No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.6493506493506493Clontech Universal Primer Mix Long (95% over 22bp)
GTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCAC10.6493506493506493No Hit
CAGCCAAGGGCTGGGCTTTGTGCCAGTGCTGGGGACATGATGTGGCCCTC10.6493506493506493No Hit
GTGTAGGGACTGGAGTTGCTGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10.6493506493506493Illumina Single End PCR Primer 1 (96% over 31bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAATACCACCGAGATCTACACTCTT10.6493506493506493Illumina Single End PCR Primer 1 (95% over 22bp)
CCAGATGATCTTGACGCTGCGCGCGGGGGACAAGGTCAGCCAGCGCGACG10.6493506493506493No Hit
CCCCTGTTCCCATGCTGACCTGTGTTTCCTCCCCAGTCATCTTTCCTGTT10.6493506493506493No Hit
CAGTGGTATCAACGCAGAGTACATGGGTGCAGTCACCTTCCTACACGACG10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGTTATCAACGCAGAGTACAGGGG10.6493506493506493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACATGGGTACGGCACACGAGATCTACCTCTTCCCTA10.6493506493506493No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTCTCTGGCCAGTATGAATGAAGTTTATACTTACCATATTTGATAG10.6493506493506493No Hit
GTGTAGGGAAAGAGTGATCTGGTGCCGATCCCATGTACTCTGCGTTGATA10.6493506493506493No Hit
TAGATTCTCTGACTGTGAAGATCATCCTGCCTGAAGGAGCCAAGAACATT10.6493506493506493No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGCTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGAT10.6493506493506493Illumina Single End PCR Primer 1 (100% over 30bp)
GAAAAACGAGCGTCGTGTAGGGAAGAGTGTAGATCCGTGGCACCCCATGT10.6493506493506493No Hit
AATGAACCAAACAATTCAAATTATCCAACACTAACATATACACATCTCAT10.6493506493506493No Hit
CACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGCTGTCTCT10.6493506493506493No Hit
CATGATAGGGACCGAGATACACTCTTTCCCTCACGACGCTCTTCCGATCT10.6493506493506493No Hit
TTCTATCGCCTGGAGACCGGCCAGCCCCCCGCCAAGCCGGCTCAGCCGGC10.6493506493506493No Hit
GTCTCGACTGGCTCCAGCTTCGAGCGGTCGACGAAATTGCGCTGGATCCA10.6493506493506493No Hit
GGAAGCAGTGGTATCAACGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
GGGTTGAACCTTCCTTTAGACAGTGCAGATTTGAAACACTTGTCTGTGGA10.6493506493506493No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GGGATTACAGGCGCCCACTACCACGCCCGGCTAATTTTTTTTTTTTGTAT10.6493506493506493No Hit
AGTTAACACTTCATGCTTCTCGCCTCCTCTTCTGCTTGGCTCTCTTTATT10.6493506493506493No Hit
GGAAGCAGTGGTATCAACGCAGAGTTGCTGGGAAAGAGTGTAGATCTCGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 25bp)
TCGTACACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ACACGACGCTCTTCCGATCTCCCTGCTGGTACCAGTAGACAGTGAGATCG10.6493506493506493No Hit
ATAACAAATAATATAACATATAAACAACTCCAACACCACAATACACACTA10.6493506493506493No Hit
TCTGGAACATCAGGAAGACCGTCTCGGGAATGGTGAAGGCCACCGCCGCG10.6493506493506493No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTTCAGGTACTAG10.6493506493506493Illumina Single End PCR Primer 1 (100% over 25bp)
AATTCGATGTACCATGTTTTTCTTTTTACGCTCTTTTGCTGCCTTTACAT10.6493506493506493No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGACCATGATGCG10.6493506493506493Illumina Single End PCR Primer 1 (100% over 36bp)
CCCGAGATCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGT10.6493506493506493Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGTAGGAAAGAACATCATGGCGGAACATTGGCCGACAACCCCATGGGA10.6493506493506493No Hit
GAGGCGGAGAGGCTGCCCAATCCCCTGGGCCTCTGCCCCAGAGCAAGCCG10.6493506493506493No Hit
CAACATGCAAGAACTGCACAACCTACAACACAATAAAAAAAACAACAACT10.6493506493506493No Hit
GTACCATGAGCTGGAAAATTTAACATATGATTTTAGGGGGACACAAGGCC10.6493506493506493No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGAAACCCCATCTCTGCAAAAAATAGCAAAAAATTAGCCGGGCATGCT10.6493506493506493No Hit
CCTTGGCGGAACGGATGCCGGAAGCGCAGCAGACCACGATCTGTCTCTTA10.6493506493506493No Hit
GTATACGGCAATATCGTGTTTTATTAATTTTTCAACTATTTTTCCATAGG10.6493506493506493No Hit
GTATTCCAGAGCATGTTAATTGTTCAGAGATAATGGACCTAACAAATAGA10.6493506493506493No Hit
CCGAAAGACGGCGGCGAAAAAATCGTCGCGTGGCTCGGGCGCGCGCTCGA10.6493506493506493No Hit
GAAGCAATGTCAGAAACTTTTATGTGATGGATCTACTCAGCTAACAGAGT10.6493506493506493No Hit
CGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG10.6493506493506493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)