FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43.7735849056603774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43.7735849056603774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAGCTCTGCGGCGGCTTCTTCCGCACCACCTGCCGCAGCTTCTTCTGG43.7735849056603774No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32.8301886792452833Clontech Universal Primer Mix Long (96% over 26bp)
GGGGCCACCCCGTGCTCGGGGCAGCAGTGCCAGAAGCACCCGTCGACGAA32.8301886792452833No Hit
ATCCGATCCGGGAGCTGGCCGGGCCCTCGCTGCGCCGGCTCGCGGAGGCC21.8867924528301887No Hit
CCGTGGACCGGGTCAGCCTCTCCCCGGACGTCACCGGGGGCCGGGAGCGG21.8867924528301887No Hit
GTCGTAGACGGTGTCGCAGGGCTGCTCGGCGTACTCCAGCCCTCCGGCGG21.8867924528301887No Hit
GTCGAGGCCTGCGCCCGCGGCGTAGCCTAGCACCGGGAAGTCAGCATCGG21.8867924528301887No Hit
CCGCATGGGCGCGGGCATCGCCCACGCCTTCCTGCTCGCCGGGGCGAGGG21.8867924528301887No Hit
GTTGTAATTATTTACATAAGCCAGGAAACTGTCGTTCTTTGCAAAGATAT21.8867924528301887No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGGTGCTCGTGGAACCGCCGCGAGATCTTCTCGTACACCGGGTCCG10.9433962264150944No Hit
AGATTGCTCTGTTCCGCGGGTTCAGCGTCAGCCCGGCGATCCGATGGGAG10.9433962264150944No Hit
GCCCTTCTTCGCCTCCCGCCAGGTGGTGTGCGTCGCTGGTCGCGTCGGCA10.9433962264150944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTACAAATGATTA10.9433962264150944No Hit
GACCGCGCTGGTCGTCCCTTCGGCGCCCGACAACGGATCCCGCTGGGAGT10.9433962264150944No Hit
GCTGTGGAACTTGACCTTGTCGGGGCGGGTCACGAACGATACATTCGCCC10.9433962264150944No Hit
AGTCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTA10.9433962264150944Illumina Single End PCR Primer 1 (96% over 29bp)
GCAACAACGTCACGACTGGGGCCGTGCGGGAGCAGATCTCCTGCGGCGTG10.9433962264150944No Hit
GCAGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAGTACTC10.9433962264150944No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGGAACGAG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
ACTGTGAGCAGCTTGGGCGGAAATGGAAGTAATCTGGAGAATTCCCAGAA10.9433962264150944No Hit
ACACCGGCCTGACCGGCCTCGCCCCGGTGTACTCGAACTTCCAGTGGCGC10.9433962264150944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9433962264150944No Hit
GTCTCGCCGCGGTGACAGAGGTCGACGTTGCTGACGCCGTCCGGGTGACC10.9433962264150944No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GGAACTGGTGCACGGCATCGACCCCGACCACCCCACCCTGTTCTGAAACC10.9433962264150944No Hit
GTTCGGGCAGCCAGTAGTCCAGCAGGCGCTGCCTTTCCCCGGCCTCCAGC10.9433962264150944No Hit
GCCCCGAAGCGGTCGGCCCGGTGGGAGCCGTGCGGCGCGGTGTCGCCCGT10.9433962264150944No Hit
GCTTCGCACCCCGGGGCACCCCGGCCAATCGTGACGGGCCGGTGACGCTG10.9433962264150944No Hit
GCATGGAAAGGGCTGATGCGGGAAGATTGTGTACATAATGATGCAGTCTT10.9433962264150944No Hit
CTCATGTTCTCCGCGGTTCTAGCGACTACCGGGGTTGGTTTGGGAAGCGT10.9433962264150944No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CGGGGGCGCCGTGTCCGCTCATGGGGTGTGGTCTCCTGTGCCGTTGGTGG10.9433962264150944No Hit
TCGGGCTGCAGGCCGCCGTCGCGGTGCAGTTCCACGAGGTGGCCTTCGCC10.9433962264150944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACCGTAAAAACCAATT10.9433962264150944No Hit
GCCCGTCGGGGCGCCGGTCCGTCAAGCCCCTCCGCGGCCGCCCGGCCGGA10.9433962264150944No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTGGGCAACGCCAAGAACTACGGCGGGCCGGTCATCGTGCACGCGAT10.9433962264150944No Hit
CGCGCGCGGCGGCGCCGCCGAGGCCGGTGAGGGCGGCGCTGGCGACGATG10.9433962264150944No Hit
GTTGTGTAATAATCAATGAGTTGACCGATGGTAATCGAATTATCCTGTCT10.9433962264150944No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTATAAGAGACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT10.9433962264150944No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GTATCATCGCAGAGTACATGGGTGATCGGCGCCACCGAGATCTACACTCT10.9433962264150944No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGA10.9433962264150944Illumina Single End PCR Primer 1 (100% over 30bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.9433962264150944Clontech Universal Primer Mix Long (96% over 25bp)
GAAGAGCGTCGTGTAGGAATGAGCCCATGTACTCTGCGTTGATACCACTG10.9433962264150944Clontech SMART CDS Primer II A (95% over 23bp)
AACTTCATGATTTAGATAGTTCAGATACAAGCAAATAAGGGATTCCAAAA10.9433962264150944No Hit
ATCCGCGATAGCCGTTGGCCTTGCACCAGCGCCGCCCCGCCTGATCCTCG10.9433962264150944No Hit
GGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 21bp)
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAA10.9433962264150944Illumina Single End PCR Primer 1 (100% over 27bp)
CCTTCCCGGTAGGCAGCGTTTCGGTGTTCCACCGATGGCCAGCCTCGCTC10.9433962264150944No Hit
CTTTAAGGCAGCAAGCGCGACCTCGCAGTGTTCAAAATGGCCATCGACGG10.9433962264150944No Hit
CCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
GATATAAAATGTGGCGTCAGGTATTACAACAATCGAGCAATACCTGTCTA10.9433962264150944No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTCGATGGGGCCGGGCAGGTTGGCCGCCCGGGAGGCGATCGTGAACT10.9433962264150944No Hit
GCCGACGACGTGCGCAACCTGATGCTCAGCCCTGCCGCCGGCCTCGACCC10.9433962264150944No Hit
GCTGCTGGACGCGGGCAAGGGCGCGGCCGCCCTGCTGATCGCACGGCATC10.9433962264150944No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.9433962264150944Clontech Universal Primer Mix Long (96% over 25bp)
TCACTGCACTGGCCGGTCTAGCTGATGCGGAAGGTCGCGCCAGGCGCCAA10.9433962264150944No Hit
GCCCTCGGCAGCGCGCGCGCCAGGTGCCAGCTGCCGCCGCCGTCGGGCGA10.9433962264150944No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.9433962264150944Illumina Single End PCR Primer 1 (100% over 25bp)
GTTGGGTAATAATCAATGAGTTGACCGATTGTAATAGAAATATCCTGTCT10.9433962264150944No Hit
GCCCGAAGATCGTCGCCATCACCGGCACCAACGGCAAGTCCACCACCACC10.9433962264150944No Hit
GCACTCCCTCGAGCCCCTGCGCCCGTGGAAGGCCGAGCAGCTCGGCGGCG10.9433962264150944No Hit
ACGCAGAGTACATGGGTCAACGCAGAGTACATGGGAATGATACGGCGACC10.9433962264150944No Hit
ATAAATGGGAAGCATTGGTAACAACGCATAGAACAAGTGAAGCAGAAGTA10.9433962264150944No Hit
GTGGGCGTGTACTCGATCATCCGCACCGAGACCGTGCTCTTCCCCGGGGA10.9433962264150944No Hit
CAGGGGGCTCGGCCTTGGCCTGCGGGGCGGGCTTGGCGACCTGAGGTTCC10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC10.9433962264150944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACGAGACTACACTCTTTCCCTACACGACGCTGTCCCAAGTTCTGCAGG10.9433962264150944Illumina Single End PCR Primer 1 (96% over 26bp)
TCTCCGGGCCCCGCACCGCGGGGTGCGCCCCGGGGTCACCGGCGGAGCGG10.9433962264150944No Hit
ACCTGGGCCTGCGCGATGCGACGCACCGGGTGCTCGCCGCGTGGGGCGGC10.9433962264150944No Hit
GGTCAAGGGCACCGAGTTCATCGACGTGCTGGAGATGTTCCTGGCCGACG10.9433962264150944No Hit
CTGCCAGGCCGTCATGCCGCCGCGGTGGCTGGGCACCGCCGGCTACGAGG10.9433962264150944No Hit
GATCTGCATCGGCGACACGGTCGCGGTTTTCGCGCAGGGCCCGATCAGGC10.9433962264150944No Hit
TCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.9433962264150944Illumina Single End PCR Primer 1 (96% over 31bp)
GTCGAGGGAGTCGTCCAGGACCTTCTCCAGACCCAGGTAGGAGAACTGCG10.9433962264150944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACG10.9433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
CTCGAACACGCCGCTGTGGACCTCGCCGGGCTCGGCATCGCCGATCGGCA10.9433962264150944No Hit
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAAGAGCGTCGTGTAGG10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph