FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42.564102564102564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTGGGTCATCCTGGCTGCGGGGTGCGCCATCGGTCTAGGTACCTACT31.9230769230769231No Hit
TCATCGAGGTGGCGTTGGTGCTGACGGTATCACTGGTGATGGTGCGGGCA31.9230769230769231No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC31.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAGTAGAAGAGCTTCAAATGAACCAGAAGAAAGGAAAAGAAATTATCCAG31.9230769230769231No Hit
GTAGGGAAAGAGTGTAGATCTCGTGTCGCACATCCCATGTACTCTGCGTT21.282051282051282Illumina Single End PCR Primer 1 (95% over 23bp)
AGCAAACACAAGTACAAAGTAAAATAGAACCACAAAATAATGAACTGCAT21.282051282051282No Hit
GGTATCAACGCAGAGTACATGGGTACGCGACCACCGAGATTACACCTGTC21.282051282051282No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTACTGGTATCG21.282051282051282Illumina Single End PCR Primer 1 (100% over 38bp)
CCTGTAATCCCAGCTACTCCTGCTGAGGAGTAGCTGGGATTACAGGCGGA21.282051282051282No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA21.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGCAGTGGTATCAACGCCTGTCTC21.282051282051282No Hit
GGACAGCGTCGTGTAGGAAAGAGTGAGTCCGGGCCCTTTCCCATGTACTC21.282051282051282No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
ATTAATCAGAAAGAACCACAAAAATAGGTTTCACTAAAAGCTAACACTGC21.282051282051282No Hit
AGACTAGAGAATGGCGATGACTTTTACCAAGTATACTACTTGTAAACATT21.282051282051282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAG21.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
CATGGGAAGCAGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAAAGT21.282051282051282No Hit
GATCGGAAGAGCGTCGTGAGGGAAATTAGATCTGTCCCCCATGTACTCTG10.641025641025641No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCATAGAACATGA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAA10.641025641025641No Hit
GGGTGCGCCCCTGCGGCACAGTTGAGGCCTGTGTCGACACCACCACCCGC10.641025641025641No Hit
GCCGCATGACCGAGACCGGGATGGTTCCTTCCCCTCTGAGCCCGGGCTTC10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCTGTTGGTAC10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATCAGTG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GCCGGGGATAGGGTAGGCATCAAACCTGCTGGTGCTGAGAACCACCCCTC10.641025641025641No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCAACAACCAAATTAGATTACTCACCATTCCCCACACTGCCTTGTG10.641025641025641No Hit
ACGCAGAGTACATGGGAAGTACGGCGACACCGAGTCTACACCTTCCCATG10.641025641025641No Hit
TGTAGGAAGAGTGTAATCTCGGGGTCGCTATCCCATGTACTCCTGTCTCT10.641025641025641No Hit
TAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTTCTACCATGTACTCTGC10.641025641025641Illumina Single End PCR Primer 1 (96% over 33bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGA10.641025641025641No Hit
AGTGGTATCAACGCAGAGTACATGGGAGAACGCGCACCGAGACACCTCTT10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGAGTTGAAACCACAGAGTCA10.641025641025641No Hit
CTTCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCA10.641025641025641Illumina Single End PCR Primer 1 (100% over 23bp)
CATCGATGATACCACAGCTACCCATGTAAACTTCGTTGACAGTCTCTTAT10.641025641025641No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CAGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
CGCGTCCACACCCCGCTGCTGCATTTCCCCCACGGCGCAGAGCTTCCGAG10.641025641025641No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGTGGTCTAACCTCGAT10.641025641025641Illumina Single End PCR Primer 1 (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
ATATAAAATGTGCCATTTTAACCATAGAGATGTACAGTTCAGGGGCACTA10.641025641025641No Hit
GAGTCGATTACGCCCACGATGTCGCCGATCTGGCGCGAGCTGGCATGGAT10.641025641025641No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
AAAGAGTGTAGACTCGGTGGTCGCCGTATCCCATGTACTCTGCGTTGATA10.641025641025641No Hit
TTGTTATTGGGACAGCCCAGCTGGGGTTGACCCACAGGATGGGGCTTAGG10.641025641025641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACAAGGGAAGATA10.641025641025641Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.641025641025641Clontech Universal Primer Mix Long (96% over 25bp)
CAACGGCAGAGTACTTTTTTTTTTTTTTTTTTTTTATGCGTTTATACCAC10.641025641025641No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACTGTCTCTTATACACATC10.641025641025641No Hit
GAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACTCTGCGTTGATAC10.641025641025641Illumina Single End PCR Primer 1 (96% over 26bp)
AGGTCTTTTCATTAGCCATTTTTACAGGCCCCCTGCTTCTAATCAACCAA10.641025641025641No Hit
ATTCCGGCCGACACCGGCGACTTCCGCGTCATGAGCCGTCGTGCCGTTGC10.641025641025641No Hit
AGTTAGCACAGACCCTGCCAGGTTAGGAGCAGGGATCCCAGCAAGTCTGC10.641025641025641No Hit
CGCTCTGTCTCCCAGGCTGGAGAGCAGTGGCGCGATCTCGGCTCACTGCA10.641025641025641No Hit
GTCGACCACCTTCCACGATCTACGCGACGGCCAGCCGTGGGAGGTGCCGC10.641025641025641No Hit
ACATGGGTAATACCGAGATCCACTCTCCCTACACGACGCTCTTCCTGTCC10.641025641025641No Hit
GATCTACACCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTAC10.641025641025641Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CCGATCTACACTCTTTCCTACACGACGCTCTCGTTTTTCAGGACCTGTTG10.641025641025641No Hit
CACGAAAAATACCAAATTACAAAAACTACCATATAACGAACTCTCTAAAA10.641025641025641No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
TAGGGAAAGAGTGTAGATCTCGGTGTCGCCCCCATGTACTCTGCGTTGAT10.641025641025641Illumina Single End PCR Primer 1 (96% over 25bp)
ACTGCGCACCTTCGGCGCTTGCGGCCTGGCGGCCGTCTTCGATCTTTGAC10.641025641025641No Hit
CCGTTTGGAGGGCCGCGGCACCCCGTCCGAGGCGAAGGAACCCCCCCAGC10.641025641025641No Hit
CTTCATGAACTCGTGAACAAACTGCTCCACTACACTGAACTCAACTGACT10.641025641025641No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.641025641025641Clontech SMART CDS Primer II A (100% over 26bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATCGGTGTTAAC10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATCTGGGTTTATACACA10.641025641025641No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTATTCTGCGT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GGGATAGAGCTTGTGACCAGAAGGTCTGAACTTTAAAAATGTCATCCAGA10.641025641025641No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATC10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAACAATGAATCATATAAAAAAAACCAAACAAAATAGAAATCAAATAC10.641025641025641No Hit
GGGAGGTAGGTTTGTGCCTTTCTCCGAAGATGATTTTGAGAGCTTCAATA10.641025641025641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.641025641025641No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACATGGGAAGC10.641025641025641Clontech Universal Primer Mix Long (95% over 24bp)
CACTGCATCCTCGAACTTCCGGGCTCAAGTGGTCCTCCTGTCTCAGCCAT10.641025641025641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATAA10.641025641025641No Hit
GCAGTGGTATCAACGCAGAATACATGGGAAGCAGTGGTATCAACGCAGAG10.641025641025641Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAAACTACATAACTTATAACCTAACACATATAAAAATATCCCAACAC10.641025641025641No Hit
ACTCAAGCCCAGTATTTACCTTTATTTCCACTCCAAGGTACTAATAATCG10.641025641025641No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGTGCGCCACGAGTCTACATCTTCCCTACACGACGCTCTTC10.641025641025641Illumina Single End PCR Primer 1 (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
AAAATGACCTGTAATCTCATCTCTCAGGTATACTTGCTAGTATATTTAAT10.641025641025641No Hit
TAGGAAAGGTGAGTCTCGGGTGCCCTCCCATGTACTCTGCGTTGATACCA10.641025641025641No Hit
CCACAAGGATGTCACGTTGTGCTTTGAACAACAGAGACGTGGCTCTGCTG10.641025641025641No Hit
TTCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAG10.641025641025641No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG10.641025641025641No Hit
CGCCTAAGGGGTTCACCTTGCCCCCTGCCTAGACAGAGCCAATTCCTCAA10.641025641025641No Hit
CAACGGCAGAGTACTTTTTTTTTTTTTTTTTTTTTATTAATTTATAACAC10.641025641025641No Hit
GTACATGGGAAGCAGTGGTGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT10.641025641025641No Hit
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT10.641025641025641Clontech SMART CDS Primer II A (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTTTAACAATGCTTCCCA10.641025641025641No Hit
CCGAGAGGGTTGCCCCTGGCACTCCAACCGGTGTTTACGCTCAGTTCCTG10.641025641025641No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATTACAT10.641025641025641Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTTAA10.641025641025641No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAG10.641025641025641Illumina Single End PCR Primer 1 (100% over 43bp)
GCGCTGAATTACGTCGTAGGCAAAGCTTTTGATTCGACAAATGGTTGCTG10.641025641025641No Hit
GGCGATCGCGAGCGCAAGGCCCAGGCCATGGCTGAGGGCGTTGAAGAGCT10.641025641025641No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GCGCGTGGATGCTCTACCGCGTCGGGGCGCTGGTCGGGTACGAGCGGCTG10.641025641025641No Hit
AAAAATAATTACAACCAAAATACAAAAAAACCCAAAAATACGAACATCAC10.641025641025641No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGT10.641025641025641Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTC10.641025641025641Clontech Universal Primer Mix Long (95% over 21bp)
AAATAAACAATTACATAAAATACTCTCATCAGATAAAATCATTCAAGCCC10.641025641025641No Hit
CTACACTAATTCCAAAAAAAAACATCTACCAAACAAATATACAAGTAATG10.641025641025641No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCAAATCTTCCCC10.641025641025641No Hit
TCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTGTGGTACCA10.641025641025641Illumina Single End PCR Primer 1 (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGTTGATACCACTGCTTC10.641025641025641No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG10.641025641025641No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATTTGCGTTGATACCACT10.641025641025641No Hit
GAATAAGAGAATGAACTAACATTTGTGAAAATAATTTCCACAAAGCATGT10.641025641025641No Hit
CTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAGA10.641025641025641No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAT10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC10.641025641025641No Hit
GTCTTAACTCATTCCAGCATTAACTTTCTTAAGAACAAAAGCTGGAATGA10.641025641025641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG10.641025641025641No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCGTACCTGTCTCTTATAC10.641025641025641Illumina Single End PCR Primer 1 (96% over 33bp)
TCGGAAGAGCGTCGTGTAGGAAAGAGTGAGATCGGTGTCCCGACTCCCAT10.641025641025641Illumina Single End PCR Primer 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph