FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGACGGCCGATACCCGAGCCCAGAATCGATCCCGAACAGACATGGGT31.9230769230769231No Hit
TTTCAACATGTTCAACGCCTCTAATATCTACAAAGACTTGTTGAATGTAA31.9230769230769231No Hit
GCCTATTTCATGTCTTTTTTAACGTCTCTTTCATATTTTCTGTCTTTCTG31.9230769230769231No Hit
AAATATGAGCATCTTACCAACAGAATCTCTTTGGTTCCTTTTAGCATGAA21.282051282051282No Hit
GATCAGACTGTTACTGTGTCTATGTAGAAAGGGAAGACAGAAGAGACTGC21.282051282051282No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT21.282051282051282Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAAGGGCCCGGACTCACTCTTTCCTACA21.282051282051282No Hit
ATGCAGTTCATTATTTTGTGGTTCTATTTTACTTTGTACTTGTGTTTGCT21.282051282051282No Hit
GCGTTGATACCACTGCTGCTTCCCATGTACTCTGCGTTGATACCTGTCTC21.282051282051282No Hit
GTGTAATCTCGGTGGTCGCGTACCCATGTACTCTGCGTTGATACCCTGTC21.282051282051282No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT21.282051282051282Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCAGAGTACTTTTTTTTTTTTTTTTTTTT21.282051282051282Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTACCAGAGATCGGAAGACGTCGTGTAGGGAGGTATCGGCCCATGTACTC21.282051282051282No Hit
GCCATTAAGTGTGGAGCTAAATAGTTCATTTTCTTCTGTGGGATAGAATA21.282051282051282No Hit
GGCCTGTTGGTACCAAAAAAGGGATAGATCGGAAGTGCGTCGTGTACGGC10.641025641025641Illumina Single End PCR Primer 1 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAT10.641025641025641No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCTGTCTCTTATACACATC10.641025641025641No Hit
ACCAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTGTCTC10.641025641025641Illumina Single End PCR Primer 1 (97% over 37bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCTTAT10.641025641025641Clontech Universal Primer Mix Long (95% over 23bp)
ACTTTTTTCAATCTCATGTATATAAAACCACCAACCTGTTTACTGACTCC10.641025641025641No Hit
CAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAGATGCGTT10.641025641025641No Hit
CCACTGCACTCCAGCCTGGGCAACAAGAGTAAAACTTCATCAAAAAAAAA10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGATACCAATGGTTAC10.641025641025641No Hit
CTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAACAGATCTACACTCTT10.641025641025641No Hit
ATTTGCTGATCATACATATGATAAGGGATTGATATCCAGAATATATAAAG10.641025641025641No Hit
GTACGGGACCACCGAGATCTACACTCTTTCCCTACACCTGTCTCTTATAC10.641025641025641Illumina Single End PCR Primer 1 (96% over 33bp)
GCTTTACCACTGGGGCCGTATCGAAGTCCGGGTTCTCCAAGGATTCGCTG10.641025641025641No Hit
GCTTCCCATGTATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.641025641025641Clontech Universal Primer Mix Long (95% over 24bp)
GTGTAGTCTCGGTGGCCCGAGCACCCATGTACTCTGCGTTGAGACCACTG10.641025641025641Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GTATCAACGCAGAGTACTGGCTGACGGCGACCACCGAGATCTACCACTTT10.641025641025641Illumina Single End PCR Primer 1 (95% over 21bp)
CTCGGAGATGTGTATAAGAGACAGGGAAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAGTAACAA10.641025641025641Clontech Universal Primer Mix Long (95% over 21bp)
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10.641025641025641No Hit
TATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCC10.641025641025641No Hit
AGATTTCACTCTACATTTTTTTTAGTTATTTGTAAAAGCAAGAAAGAAAA10.641025641025641No Hit
GAGTACATGGGAAGCATGGTATCAACGCAGAGTACATGGGAAGCATGGTA10.641025641025641No Hit
GCTCCGGGCCGGGCGTTCACGCGGGAGTCGGACGGAGCCTCCTGCCGCCT10.641025641025641No Hit
GGGAAAACAAGACCCTGTCTGTACAAAAAATTAAAAAAGCTGGGTGTGGT10.641025641025641No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
CATGGGAAGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAATT10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAGTATCAA10.641025641025641Clontech Universal Primer Mix Long (95% over 21bp)
GTAGGCAGGCACTCTCGGCTCCCGCCCCGACACACTCATTGCGCACGGAC10.641025641025641No Hit
AGAATCAGGAGGGCAAAGAAAAGAAATATGGGAAGTAGACCGGCGGAGAA10.641025641025641No Hit
AGGCAGTGATGCCATCCACGGCAAAACGCAACAGCTTGCGGTAGTTGAAC10.641025641025641No Hit
GTATCAACGCAGAGTACATGGGTATACGGCGACCACCGAGATCTACACTC10.641025641025641Illumina Single End PCR Primer 1 (100% over 27bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGGCGTTGAAACC10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGCGGCACCA10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.641025641025641Illumina Single End PCR Primer 1 (100% over 25bp)
GTACATGGGAGCAGTGGTATCAACGCAGAATACATGGGAAGCAGTGGTAT10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTATCCCTTTTTTGGCCTGTTGGTACCAAAAAGGGATAGATC10.641025641025641No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.641025641025641No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAGTACTTTTTTTTTTTTTTTT10.641025641025641Clontech SMART CDS Primer II A (96% over 26bp)
CCAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGGTGTAATCTCGGGGC10.641025641025641Illumina Single End PCR Primer 1 (96% over 28bp)
GTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGG10.641025641025641No Hit
GTAGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTATTCTG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGGAAGAGCGTCGTGTA10.641025641025641No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACATCTTACCA10.641025641025641No Hit
AAATTCCAGTGATATTTCAGTTCCTGGTGTAGATCAGCAGAGCCGGCCCA10.641025641025641No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
TCCGATCTCTGGTACCTGTAGAACTTGGGACAGGTACCAGAGATCGGAAG10.641025641025641No Hit
GGTATCAACGCAGAGTACATGGGTGTACGGCGCCACCGAGATACACTCCT10.641025641025641No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.641025641025641Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAGGTCGATCCGGCCTGGGCTACGAGGTTCGGCACGGACCGCATGCT10.641025641025641No Hit
GTACATGGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.641025641025641Clontech Universal Primer Mix Long (100% over 27bp)
GGCTTCTGGCAGGCTCATGACCCCTAGCAGCTCGCTGAGGAGTGAGGGCC10.641025641025641No Hit
TTCCCTACACGACGCTCTTCCGATCTCACTGTCCCTGCTGGTACCAGTAG10.641025641025641Illumina Single End PCR Primer 1 (100% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCAATGCTTCCCA10.641025641025641No Hit
GAACTGAGCGTAAACACCGGTTGGAGTGCCAGGGGCAACCCTCTCGGCTG10.641025641025641No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATACAA10.641025641025641Clontech Universal Primer Mix Long (95% over 21bp)
AACAACTTCAAAGGCCACCAGTTTGAGGAGGACTGGATGGAGTGACGTCA10.641025641025641No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGGGTTTTTA10.641025641025641No Hit
GTATCAACGCAGAGTACAGGGGTATACGGCGACCACCGAGATCTACACTC10.641025641025641Illumina Single End PCR Primer 1 (100% over 27bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGACGCTTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGG10.641025641025641No Hit
GAGTACATGGGATAGCGACCCCGAGATTACACTCTTCCTACACTGTCTCT10.641025641025641No Hit
GCAATGTAGGTAGGGTTAGTTGGTAGGAGGAGAAGCCATCCTTGAAATCG10.641025641025641No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGTGCGCGACCCCGAGATCTACACTCTTCCTACACGAC10.641025641025641No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGAATAACTCGTTGTTCGCTTA10.641025641025641Illumina Single End PCR Primer 1 (96% over 29bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.641025641025641Illumina Single End PCR Primer 1 (100% over 30bp)
CGGCTCTGTGTGAATTACTCTTTCTCTGTTGCAATTCCCACATCTTGAGG10.641025641025641No Hit
CGAGAGCGAAACTCCATTTCAAAAAAAGAAAGAAAAAAAAAAAAAAAAAA10.641025641025641No Hit
AATCTGAGGCAAGAGCGTCGTGTAGGGAAAGAGGTGTCTCGGTGCGCGTT10.641025641025641Illumina Single End PCR Primer 1 (95% over 23bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.641025641025641Clontech Universal Primer Mix Long (96% over 28bp)
AGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC10.641025641025641No Hit
GCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
GAATAGGCGACCCCGAGATCTACACTCTTCCCTACACGACGCTCTTCCGA10.641025641025641Illumina Single End PCR Primer 1 (95% over 24bp)
GGAAGTAGTGGGCGCCATGCAGGACATCCATGCCAGCTCGCGCCAGATCG10.641025641025641No Hit
CATCCCTTCTTAATAGTCCCCCAAAGTCTTAACTCATTCCAGCTTTTGTT10.641025641025641No Hit
GGCCAACATGGTGAAACCCTGTCTCTAATAAAAATTCAAAAATTAGCTGG10.641025641025641No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGATCTCGGAGAAAAAAAAAAA10.641025641025641Illumina Single End PCR Primer 1 (96% over 29bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTGATACCA10.641025641025641No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAGAACGGCGAC10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCCTATATTATTAAGCACGTGACCAACATGCTTTGTGGAAATTATTTTC10.641025641025641No Hit
GTACATGGGAGTCGGGACACCGATCTCACTCTTTCCTACACGACGCTCTT10.641025641025641No Hit
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAA10.641025641025641Illumina Single End PCR Primer 1 (100% over 27bp)
GCGCGCGCACGCGGCGCAGCAGTTGCAGCTTGAGCTTGTCCACCATCAGG10.641025641025641No Hit
TGTAAAATCCCTATGTAAACTCTAGAGCCCCATATCATTGCAATTGTGTT10.641025641025641No Hit
GCGGTGAGCAGGCTGAAGCGCAGAAGCGGCATGCCGGTGAGCCCGGCCGG10.641025641025641No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACC10.641025641025641Clontech Universal Primer Mix Long (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAA10.641025641025641No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCACGGCTT10.641025641025641No Hit
GTGGTTTGGCAGAGCCAGCAGGAAAGCATGGAGGGGTGGTTCTCAGCACC10.641025641025641No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTATACCACTGGGTCC10.641025641025641No Hit
ATTACTAGCTGCTAAAACCACCAAAACCGAGCGGCGATTATTAGTACCTT10.641025641025641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATGCGTT10.641025641025641No Hit
GAGAGAAGACGCAGGAGCCCAGTGTCCTGGACCCTCCTGGCCTTCCCCTA10.641025641025641No Hit
GTGGGAGCAGCAACCATTTGTCGAATCAAAAGCTTTGCCTACGACGTAAT10.641025641025641No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.641025641025641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGGCTCCAGCAAGTTTCCTCCCATTAAGTGGGAGGAAACTTGCTGGA10.641025641025641No Hit
GTGGTCGCCGTATCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.641025641025641Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCGTCGTGTAGGAAAGATGTAGACGGTGGTCGCCCATGTA10.641025641025641Illumina Single End PCR Primer 1 (96% over 25bp)
GTTGTATTCTTTTGAGTCTTTGATCAGCCTTTCACTGAATACACAATATA10.641025641025641No Hit
GTACATGGGTGGCCCCCGAGTTACCTTTCCCTACACGACGCTCTCCGATC10.641025641025641Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph