FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73.482587064676617Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41.9900497512437811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT41.9900497512437811Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41.9900497512437811Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41.9900497512437811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGCTGTCTCTTA31.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGCCCCACGAGCTGGGCGCCGTGTTCCGCCCGTCGGAGCCGAACGGCC31.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT31.4925373134328357Clontech SMART CDS Primer II A (100% over 26bp)
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACAAACCTCTGGTGTACC31.4925373134328357No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTGGGCCGAACCCCCTCGGGCCAGCTTTTCAGGAAGCGCTCCAGCGT31.4925373134328357No Hit
GCACGAGCACCACCACGATCAGCACGGCCAACCGGCCGAACAGCGTGTCG20.9950248756218906No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.9950248756218906Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAATGCCACCG20.9950248756218906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCAGAGCATCCCGTACCAGGTCATGGCCGCGACACCGAACGCCGTACG20.9950248756218906No Hit
GGTGGAGGGTCTACGCGGCCGAAGGGAGAAGGGGGCGGGCGAGAAGGTCC20.9950248756218906No Hit
ATACGCAGGTGCAACCATCCCAAGACGTACCGATCCGGAGATAGCGGCCA20.9950248756218906No Hit
CCCATGTACTCTGCGTTGATACCACTGCTATCAACGCAGAGTACTTTTTT20.9950248756218906Clontech SMART CDS Primer II A (96% over 25bp)
GTGGCGAGCTATGTCGCGTGAGGTCGTGGATCCGGCCCGGCTCGAGGAGG20.9950248756218906No Hit
GTGGGAAAGAGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.9950248756218906Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.9950248756218906Clontech Universal Primer Mix Long (96% over 26bp)
GCACGCGGATGCGCAGGCGCTGTCGTATCACCGGCCCTGATCGAGCCCTG20.9950248756218906No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGCGCCACGATCTACACCTG20.9950248756218906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCGATACGCGGCTGGCCATCGCAGGGCGCCACAACGTCACCAATGCCCT20.9950248756218906No Hit
TTCTAGTAGAGTATCTAGTCGGCGTAAGACCACCATTGGCAGAATCACAT20.9950248756218906No Hit
CCATTGGGAACTGACCGGGCTGCGGGTGAACGTTCAGGAACGCCTGTACG20.9950248756218906No Hit
CCGTTCAGCCGCTCGCGGCGACGCAGGCCCTCGCGGGCGATGGCGACCAC20.9950248756218906No Hit
GGATATAGCAGCCACGAACCCACCTCGACGTCGAGGGTCCCGACGTCGAG10.4975124378109453No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAA10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAA10.4975124378109453No Hit
TTCCAGGTGATCGCGCTTCCGGTCTCGACCTGGGTCCAGCTGACCTTCGA10.4975124378109453No Hit
GGAAGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.4975124378109453No Hit
GGTACACGCCGTGCCCGCGCCGGGCATCGCCGATTGCCGGCGCGTCGAGC10.4975124378109453No Hit
CTCTTCCGATCTGGTACCAACAGTCCTGAAAAACGAGAGCGTCGTGTAGG10.4975124378109453No Hit
GTCGGCATCGTCCCGTTCGTCATCACCGTCCCGCTGTCGCTGCAGTACGT10.4975124378109453No Hit
GTGTAGGGAAAGAGTGTGATTCGGGTCGTCATTCCCATGTACTCCTGTCT10.4975124378109453No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACA10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATCATCCAACGCCCGTGCTGCAACGCGGCTCAGGTTTCTCCGGCAAC10.4975124378109453No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTATGCGTTGATAC10.4975124378109453No Hit
GTAAAAAGGTAATTTCAATTCCTGTGTGGTGCGATTAAGAGTCTTGTTTC10.4975124378109453No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGCGCCGCCCCAATGCGAACCCACGCGGGAAGGACCTCACTGGCGG10.4975124378109453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.4975124378109453No Hit
CAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGT10.4975124378109453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.4975124378109453No Hit
GCAGAGTACATGGGCGCGAATTACCTTTCCCTACACGACGCTCTTCCGAT10.4975124378109453Illumina Single End PCR Primer 1 (96% over 27bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACCC10.4975124378109453No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGTGTAT10.4975124378109453No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACC10.4975124378109453No Hit
GACCATGGTCGGCATCTACAACGTCACCGAGTCCGTGCAGTCGCTGCCGA10.4975124378109453No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGTGTTGATAC10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTATGAAGCCCTGTCGTCGGCTCATCCAAGATATAGAAGGACTTACCAGT10.4975124378109453No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAATG10.4975124378109453No Hit
CTACGCCAGCGCCGAAGACGGCGAAGTCTTGCGCTCGCGCGGGCATCGCG10.4975124378109453No Hit
GTACATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA10.4975124378109453Illumina Single End PCR Primer 1 (100% over 41bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGCGTTTATA10.4975124378109453No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.4975124378109453Clontech Universal Primer Mix Long (96% over 25bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGACCATGATGCGGGG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 33bp)
CCGCGAGCCCGTGCCAGCCGCGGGCGTCGTCGCGGGGGACCAGGTACTCG10.4975124378109453No Hit
GTACATGGGAATGATACGGCGACCACCGAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453Illumina Single End PCR Primer 1 (95% over 22bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAATACTTTCTG10.4975124378109453Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTGAGTTTTTTCCCAGGGGGAGTGGGT10.4975124378109453No Hit
GAGTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCCCCATGTACTC10.4975124378109453No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACAGCACGGCACCGTCGTTCGAGCTGGTCGACTGGGACGCGGTGGTCG10.4975124378109453No Hit
TATCAACGCAGAGTACATGGGTGATACGGCGACCACCGAGATCTACACTC10.4975124378109453Illumina Single End PCR Primer 1 (100% over 29bp)
GTCTACAAGATCTACGCCGAATCGTTCCGCGGTCCCGAGCACCTGGCCCA10.4975124378109453No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGGTGCGTTTATACC10.4975124378109453No Hit
ATGCTCTTCCGATCTCTGGTACCTGCTGTCCCAAGTTCTGCAGGTACCAG10.4975124378109453No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA10.4975124378109453Illumina Single End PCR Primer 1 (100% over 36bp)
AAATAGGCGTCCGACTCCGCCGCCGGGACGCGACTCACCGTGCCCTCGAT10.4975124378109453No Hit
ACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 22bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGA10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCATGCATAATTAGTTTACCTGCTGTCACGTCAAGCTGTTGAACTTG10.4975124378109453No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGCAGG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTGATACGGCGAC10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCACAGGGTCCC10.4975124378109453No Hit
GTATAGATCTCGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC10.4975124378109453No Hit
GATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTACGCAGAGTA10.4975124378109453Clontech SMART CDS Primer II A (96% over 25bp)
GATGTACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT10.4975124378109453Illumina Single End PCR Primer 1 (100% over 46bp)
GGAAGACACAGATGCTCATCTTTCTAAGTGCTTTCATGTTAGTATTTCAA10.4975124378109453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGTCCCCGGGGATGACGAACCGGGCGGCGGCACGGTCGGCCAGCCGCTC10.4975124378109453No Hit
GTGCCGCCCGCATTCACGTTGCCGGTGACGTTGACCGTGCCCGCATTCAG10.4975124378109453No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATCTCGGGGTCTGTCTC10.4975124378109453Illumina Single End PCR Primer 1 (96% over 33bp)
TTCGGTGACCGAGCAGGAAATTGCCCAGCGGGTGGCGCATCACCGCGGTT10.4975124378109453No Hit
TGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453No Hit
GGTTACGGCGACACCGAGATCTCACTCTTTCCTACACGACGCTTGTAAAA10.4975124378109453No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCG10.4975124378109453No Hit
CTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.4975124378109453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTAT10.4975124378109453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAACTGGTGCCCAAGTA10.4975124378109453No Hit
TACCATGACATCACAGGGGTATGCATTCAGCAAGATTCGCTATTCCGCAA10.4975124378109453No Hit
AAAAAGTACTCTGCGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.4975124378109453No Hit
CCCATGTACTCTGCGTTGATACCACATGGGAAGCAGTGGTATCAACGCAG10.4975124378109453Clontech SMART CDS Primer II A (95% over 21bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAC10.4975124378109453No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GCCACTAACTGTGTGGTTTCAAGCTTGGCTATGATGTTTTTTTCACACTG10.4975124378109453No Hit
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA10.4975124378109453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
CACTCTTTCCCTACACGACGCTCTCCGATCTCTGGTACCGACAGAATCTG10.4975124378109453Illumina Single End PCR Primer 1 (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTACACCGGCGCCACCGAGCTGCCGGAAGACTTCTGGAAGCGCCACG10.4975124378109453No Hit
TTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT10.4975124378109453Clontech SMART CDS Primer II A (100% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4975124378109453Clontech SMART CDS Primer II A (100% over 23bp)
ACACAGCAACTCCAGTCCTTGCATCATGAGATCGGGAGAGCGTCGTGTAG10.4975124378109453Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTGATA10.4975124378109453No Hit
CAACAACTCTCGCTGCGAGCGGACTGAAACGTACTCGCCGGCGCCCATCG10.4975124378109453No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATTC10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGTCGCACCGAGACTAACACTTTCCCT10.4975124378109453No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTAAACCAAATCTAAC10.4975124378109453No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.4975124378109453Clontech Universal Primer Mix Long (96% over 25bp)
GCATGCGCAGCCGGTATTCCCGCGTGTTGTGCAGCTCGGGCATCCGGACC10.4975124378109453No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.4975124378109453No Hit
GTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACCCAGAGTACATGG10.4975124378109453No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGTCGCACA10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTCGCGCACGAGATCTACACTCTTTCCCTACACCTGTCTCTT10.4975124378109453Illumina Single End PCR Primer 1 (96% over 25bp)
GGATACGCGACCGAGATACACTCTTCCCACACGACGCTCTTCCGATCTCT10.4975124378109453Illumina Single End PCR Primer 1 (95% over 23bp)
TGCTGGGCGTGATCGGCCCCAGCCGCATGGCCTACGAGCGGGTGATCCCG10.4975124378109453No Hit
CTCGCCACCTGCAAAGGCCTCCCGCTGGCCTACAACCGTGATCTGCAGGA10.4975124378109453No Hit
GGATATAGCAGCCACGAACCCACCACGACGTCGAGGGTCCCGACGTCGAG10.4975124378109453No Hit
GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTGTA10.4975124378109453No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.4975124378109453Clontech Universal Primer Mix Long (96% over 25bp)
AGTTCACGCACAGCTGGGCGGTCCCGCCCGGGTAATGCAGGCTGGTGGCC10.4975124378109453No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTC10.4975124378109453Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGG10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATAGACCGATCTCTCTTTCCCTACACGACGCTCTTCCGATC10.4975124378109453Illumina Single End PCR Primer 1 (96% over 31bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGACCAC10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
CTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGA10.4975124378109453Illumina Single End PCR Primer 1 (100% over 28bp)
NAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTTTTTAC10.4975124378109453No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTCGTTTTTACCACTTATAGCGGTG10.4975124378109453No Hit
GGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACAT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATCTACACTCTCCCTACACGACGCTCTTCCTGTCTCTTATA10.4975124378109453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAGAGTACATGG10.4975124378109453Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 22bp)
ATTTTCAATTTTCTTGCCATATTCCACGTCCTTCAGTGGAATATGGCAAG10.4975124378109453No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAC10.4975124378109453No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTACAC10.4975124378109453Clontech Universal Primer Mix Long (95% over 21bp)
GTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAGGG10.4975124378109453Illumina Single End PCR Primer 1 (100% over 24bp)
TCTGAGGCAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTGGTGGTCGCG10.4975124378109453Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)