FastQCFastQC Report
Tue 10 May 2022
EGAF00003666109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10687
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA230.21521474688874334No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA230.21521474688874334No Hit
TCTATGATCTGTCCCTGCTCCAATTTGTAAAAAACGCAAGATTCATCAGA210.19650042107233087No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA190.1777860952559184No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG190.1777860952559184No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG170.15907176943950593No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT160.14971460653129973No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG160.14971460653129973No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT160.14971460653129973No Hit
GTACATGGGATGTCACCCCCATCAGATAATGTGTTTCATCCACAGAAGGG150.14035744362309346No Hit
GCTATGCATTGCATGGCATCCGCTACAAGAACGACCATATCAACCTGGCC150.14035744362309346No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA140.13100028071488726No Hit
ATTTTATTGAGTGGAAAGCTTACAAAAGGTCCACTGGCCCCTTCCCTCCC140.13100028071488726No Hit
GTACATGGGAGGTGTTTTTCCAGGTCTGACCACCTGGCCCTGCACATGAA140.13100028071488726No Hit
ACCTACACGTGTCCGTGGAGTCCCGTGGCCAGCCTGTCAAGATCTATGCC140.13100028071488726No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG140.13100028071488726No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT140.13100028071488726No Hit
GTACATGGGAACCTGGGAAGGAATTCAGGCTGGAAAGGAGCTCGAGGAGC140.13100028071488726No Hit
GTACATGGGAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCA140.13100028071488726No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA140.13100028071488726No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG130.12164311780668102No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA130.12164311780668102No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTATTT120.11228595489847477No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT120.11228595489847477No Hit
GTACTAGGCATCGTCATCCAATGCAACGAGTCCTACACTATCTTGGATAT120.11228595489847477No Hit
TATCAACGCAGAGTACATGGGAACAGCTATGCATTGCATGGCATCCGCTA120.11228595489847477No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT120.11228595489847477No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC120.11228595489847477No Hit
GTACATGGGCGGTCGCGCTGGGCAACAAGGTCTCGCTCGCGGGCATCGGG120.11228595489847477No Hit
GTATCAACGCAGAGTACATGGGAAGGGATCAACCTTTCCCGGCAAGGCGG120.11228595489847477No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT120.11228595489847477No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG120.11228595489847477No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA110.10292879199026855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTT110.10292879199026855No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT110.10292879199026855No Hit
GTACATGGGATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTG110.10292879199026855No Hit
GTATCAACGCAGAGTACATGGGATAGAAGCCGATACCGTGAAAGAAGAAA110.10292879199026855No Hit
TATCAACGCAGAGTACATGGGAACCTGGGAAGGAATTCAGGCTGGAAAGG110.10292879199026855No Hit
GTCATGGGCGCCTCCTTCCGCAACACGGGCGAGATCAAAGCACTGGCCGG110.10292879199026855No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC110.10292879199026855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)