FastQCFastQC Report
Tue 10 May 2022
EGAF00003666110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10687
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA440.4117151679610742No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG200.18714325816412464No Hit
TCTATGATCTGTCCCTGCTCCAATTTGTAAAAAACGCAAGATTCATCAGA190.1777860952559184No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA190.1777860952559184No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT190.1777860952559184No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT170.15907176943950593No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC160.14971460653129973No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG160.14971460653129973No Hit
GTACATGGGAGGTGTTTTTCCAGGTCTGACCACCTGGCCCTGCACATGAA150.14035744362309346No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA140.13100028071488726No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG140.13100028071488726No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA140.13100028071488726No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT140.13100028071488726No Hit
TCTCTACCTCTTCCCCAACGGCTCGCGGCTCGACGATTCGATCGAGGCGG140.13100028071488726No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT130.12164311780668102No Hit
ATTTTATTGAGTGGAAAGCTTACAAAAGGTCCACTGGCCCCTTCCCTCCC130.12164311780668102No Hit
GTACTAGGCATCGTCATCCAATGCAACGAGTCCTACACTATCTTGGATAT130.12164311780668102No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC120.11228595489847477No Hit
GCTTTAATGTATTAGGCAAAATTTTACATAAAATCAGAAATCTATGATCT120.11228595489847477No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT120.11228595489847477No Hit
GTACATGGGAACCTGGGAAGGAATTCAGGCTGGAAAGGAGCTCGAGGAGC120.11228595489847477No Hit
GACCATAATGTGATCCCTTCCGGCGATCGGTATAAAATCAGGCAGTTTTT120.11228595489847477No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC120.11228595489847477No Hit
TATCAACGCAGAGTACATGGGCCGCGGCAAGAATGCCGCCATGCTGATCC110.10292879199026855No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA110.10292879199026855No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA110.10292879199026855No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110.10292879199026855Clontech Universal Primer Mix Long (96% over 26bp)
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT110.10292879199026855No Hit
GTACATGGGAGCCTCTTCAGGCGCAGAACTTGATCTCCAGGCGGCCTTTG110.10292879199026855No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC110.10292879199026855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)