FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGACACCCTAACATCACAATTAAAGGAACTAGAGAAGCAAGGGCAAAC26.896551724137931No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC26.896551724137931No Hit
GCCTGGAGAGGGGTAAGCAGCAGGATGGGCACAGTGGAGAGAGAGAACTG26.896551724137931No Hit
TGTCTAACCCAAGTAAATAAAACCTATGAGACACAAAGTAGCCAAAAACA26.896551724137931No Hit
GAACCGCACAGAGCCGCGGGCCCTTTGCAGCTGCGATTTTCGCCCTACGT26.896551724137931No Hit
TTCCTACACGACGCTCTTCGATCTCTGTCCCAAGTTCTGCAGGTACCAGA26.896551724137931No Hit
GAGCGGCGTGATGCGCAGGATCATGGCGGACCGCCTCAGGCGTTCGCCCG26.896551724137931No Hit
CCATAACACGATCTAGTGAAACCTTCAAGCATGAATTGTAGCTGACGGCA13.4482758620689653No Hit
AGCTGGGCCTCTGCGCCCGGCCCGACCTCCGTCTATAAATAGAGCAGCCA13.4482758620689653No Hit
GAACTGGGCAATGACCAGCCTGGGCAACATGGTGAGACCCTATCTTTACA13.4482758620689653No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCTGTCTCTTATACACATCTC13.4482758620689653Illumina Single End PCR Primer 1 (96% over 31bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGTAG13.4482758620689653No Hit
CTAGCTGGATGTCTTTTGGCATAATTGTTACACGTTTGGCATGGATAGCA13.4482758620689653No Hit
TCCTTGTTGGACTCCAGTCGGAACCAATCGTTGTCAGCATTCTTGTTGTT13.4482758620689653No Hit
CAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAG13.4482758620689653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.4482758620689653No Hit
GCCTCGGTTAAATAGAGAGTGAAGAATGCTGGACAGGAACCTCCACCCTC13.4482758620689653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.4482758620689653No Hit
CCCTTCCACTGCATTCCATTCGATTCCATTCCATTGCATTGCATTCTATT13.4482758620689653No Hit
CCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTAC13.4482758620689653No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA13.4482758620689653Illumina PCR Primer Index 1 (96% over 28bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA13.4482758620689653No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)