FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTA26.896551724137931No Hit
TTATTGGAATGCTTATACATCTTTAGTCTTGCTTCAATAATCTTTGTCAG26.896551724137931No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG26.896551724137931No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26.896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCAGAATCGTCGCGGGGAGCCACGACGACGAGCCATAGCTAAAAGAAC26.896551724137931No Hit
GTATTAGGAGGTGGGGCTCAGGAAGATAATTAGTTCATGAGGGTCGTGCT26.896551724137931No Hit
GGAAGTTTAAAATCAAAGACTCCTGGGTCTGGGGACTCAAAGCACCCAAA26.896551724137931No Hit
CCTTTATCATTTTTTATTGCGTCTATTTGATAGTTCTCTCTTTTCTTCTT13.4482758620689653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTA13.4482758620689653No Hit
GAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT13.4482758620689653No Hit
TTAAAACAGATCTGCGCTTCCAGAGCGCAGCTATCGGTGCTTTGCAGGAG13.4482758620689653No Hit
GGCGGTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCG13.4482758620689653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGCA13.4482758620689653No Hit
GACTCAAATAGAATGGACTTAAAAGAAAAGGTCTTGAATGGAACTAATAC13.4482758620689653No Hit
GGCAGCAGGCAGAGAGCCTTTACTGTCATAGTGAAGAGCTTGCCAAGGAC13.4482758620689653No Hit
CTTTCACTCAGATGGGAATGCACTGGCATGGCCTTGGCAGCCTCGAACTC13.4482758620689653No Hit
TAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGC13.4482758620689653No Hit
CCTTTATCATTTTTTATTGCGTCTATTTGATACGTCTCTCTTTTCTTCTT13.4482758620689653No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGTCTCTTATACACATCTG13.4482758620689653Illumina Single End PCR Primer 1 (96% over 30bp)
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG13.4482758620689653Illumina PCR Primer Index 1 (96% over 28bp)
GGTATCAACGCAGAGTACATGGGGTCCAAGATGGCGGATGAAGCCACGCG13.4482758620689653No Hit
GGCACACTTGGCACAGCCCACGGGGCAGCAGGAACAGCAGCTCTTCTTGC13.4482758620689653No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)