FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39.67741935483871Clontech Universal Primer Mix Long (96% over 26bp)
CCATTCCCTGTCCCTCGGCCCCCTGAGTGAGTCCGGTCTCCCGGCGAAAG26.451612903225806No Hit
GTGCCGGAGAAGGGACTCGAACCCTCACACCACAAGGCACAGGAACCTAA26.451612903225806No Hit
TTTTCAGTTCCTTCATCTCCCTATTTGACAGTTTCTTGACATTTCACCTT26.451612903225806No Hit
GTCCAGAATCAGATGGATTCACAGCCAAATTCTACCAGAGGTACAAAAAG26.451612903225806No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC26.451612903225806No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGAACATAGTCCTCTGAAATAACACCACACAGCTACAACAATCTGATC13.225806451612903No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGATTAGACCGTC13.225806451612903No Hit
CTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTCCCAGCTCATTTA13.225806451612903No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTG13.225806451612903No Hit
CTATTATTATTCCCGTCATACAGATGAGGAAAGAGAGGAAGAGAAAAATT13.225806451612903No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13.225806451612903Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGACTTCAGTGAGTTCTGATGTGTGCTTTTTGCAAATACATGTTCTC13.225806451612903No Hit
AAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGA13.225806451612903No Hit
GTACTTTTTTTTTTTTTTTTAGTCGTGCACCTGGCTTGTCCCCGAGCGTA13.225806451612903No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTC13.225806451612903No Hit
CTGCGATGCTGAAACCTGGCCTACCCAGCACCACATAGAAGTCCAGGCCG13.225806451612903No Hit
GTTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGGTGGTGAT13.225806451612903No Hit
GGTATCAACGCAGAGTACATGGGATGGAGTCTAGGAGAGACCAAGCGTGA13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)