FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA412.903225806451612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGATACTAGCTGATTATTTAAGGAAGAAGGCTATTTAATGAGAGGAGG26.451612903225806No Hit
GTACAATGTTCGAGCCAAGGGTCATGTCGACAAGCTCTGCAAGGAGCTTA26.451612903225806No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26.451612903225806Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGATGTTTAGCTAAATCTATAGTGCTTCTTGATGCTCTTAGTTGTT26.451612903225806No Hit
ATGTTCATCAAGGATATTGGTCTAAAATTCTCTTTTTTTTGTTGTGTCTC26.451612903225806No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13.225806451612903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA13.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
AAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTC13.225806451612903No Hit
GGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTT13.225806451612903No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13.225806451612903Clontech Universal Primer Mix Long (100% over 23bp)
GCTGTGTCCTGTAATAAGAGCATCACATGAGATGTGGAGAAGGAAAACAT13.225806451612903No Hit
GGGAGAGAGAGAGAGCAAGAGAGCGAGCTGTGAGCTGTGCTGCCGCCGCC13.225806451612903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTATGC13.225806451612903No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGC13.225806451612903No Hit
GGGTACCACGGCGTGGGTGAGCTTCTCTGGCAACACTTTGTGTGCGGTCA13.225806451612903No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACATTGTTGTTGAGG13.225806451612903No Hit
TAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACAT13.225806451612903No Hit
CAGCTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCC13.225806451612903No Hit
CCTGAATGGTATTGCCTAGGTTTTCTTCTAGGGTTTTTATGGTGTTAGGT13.225806451612903No Hit
GGCAGAAGAAATCTTGGAGAAGGGTCTAAAGGTGCGGGAGTATGAGTTAA13.225806451612903No Hit
AAGTTTGTACATTTTGCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTT13.225806451612903No Hit
ATGCCATGATGTTGCCCTAAGCTTAGGGTATTGAAGTTGAAGCAGAAGTT13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)