FastQCFastQC Report
Tue 10 May 2022
EGAF00003666277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430961
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG16860.3912186949631173No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT13420.31139708697538754No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC12810.29724267393105175No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG12220.28355234000292373No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA9970.23134343942955393No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG9320.2162608681528027No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC9300.21579678903659497No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG8130.18864816073844268No Hit
CTACAGCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGC8100.1879520420641311No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC7910.18354329046015766No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA7600.1763500641589378No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG7340.1703170356482373No Hit
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG7230.16776460050909478No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA7200.1670684818347832No Hit
ACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTC6880.1596432159754595No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG6670.15477038525527834No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA6600.15314610834855125No Hit
ACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTT6400.14850531718647395No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG6270.14548880293112368No Hit
TCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGT6110.14177617000146187No Hit
GTACATGGGAGGGACCCGCAGCTCAGCTACAGCACAGATCAGCACCATGA6010.1394557744204232No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC5920.1373674183974884No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA5900.13690333928128068No Hit
AGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAG5860.13597518104886522No Hit
CCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCCACA5720.1327266272354111No Hit
CCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCC5680.13179846900299563No Hit
GTGTACCCTCTCCCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAG5670.13156642944489177No Hit
GAGAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAA5580.129478073421957No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC5400.1253013613760874No Hit
GAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGC5310.1232130053531526No Hit
GTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAA5310.1232130053531526No Hit
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA5170.11996445153969848No Hit
GGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACA5160.11973241198159462No Hit
GTCCTGACACTTGTGGCATCAAATGCCTGGATCCTGTTGACACCCCAAAC4740.10998675054123228No Hit
GTCTCAGGGTGGAAAGGACCTGGACCACACAGAGCAGGACTCCAGAGCCT4720.10952267142502455No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG4610.10697023628588201No Hit
GACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCAT4560.10581003849536269No Hit
TATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACACAG4450.10325760335622017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)