FastQCFastQC Report
Tue 10 May 2022
EGAF00003666278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430961
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG15430.3580370381542645No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT13120.30443590023227163No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC12600.29236984321087056No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG11660.27055812474910723No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA9160.2125482352231408No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC9090.21092395831641378No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG8860.20558704848002485No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC8440.19584138703966253No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG8010.18586368604119632No Hit
CTACAGCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGC7780.1805267762048074No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG7600.1763500641589378No Hit
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG7520.1744937476941069No Hit
ACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTC7410.17194131255496437No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA7380.1712451938806528No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA7240.16799664006719867No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA6820.15825097862683632No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG6560.15221795011613581No Hit
ACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTT6370.14780919851216234No Hit
TCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGT6230.14456064469870825No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG6170.14316840735008504No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC5940.13783149751369614No Hit
GTGTACCCTCTCCCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAG5780.1341188645840343No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC5750.1334227459097227No Hit
CCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCCACA5650.13110235032868403No Hit
GTACATGGGAGGGACCCGCAGCTCAGCTACAGCACAGATCAGCACCATGA5630.13063827121247631No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA5600.1299421525381647No Hit
GAGAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAA5390.12506932181798353No Hit
GTCCTGACACTTGTGGCATCAAATGCCTGGATCCTGTTGACACCCCAAAC5380.12483728225987965No Hit
CCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCC5350.12414116358556807No Hit
AGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAG5310.1232130053531526No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG5220.12112464933021783No Hit
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA5190.12042853065590622No Hit
GAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGC5140.11926833286538688No Hit
GTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAA5110.11857221419107529No Hit
TATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACACAG5060.11741201640055596No Hit
GGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACA4960.11509162081951732No Hit
GACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCAT4720.10952267142502455No Hit
GCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTT4520.10488188026294722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)