FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003666280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTTTACCTTTATACCAAGAAGGTTGGGAAAGCACCAAAATCTGCATG42.7586206896551726No Hit
GGTTGTGCGGCCTGCACTCCTTGTCCCACAGCTGGCATTTTTCCTCTGCC32.0689655172413794No Hit
CAATAATGCAGAGTGAGGTTGGTAGGCTGGGGCTCAGAGCTGCTGTTCCA32.0689655172413794No Hit
AGTAATTTACAAAAGCAGGTTTCAGTGAAGCCATCTGGTTGTTACCCTTG32.0689655172413794No Hit
GTACATGGGACTTCCTGTGAGCCCGGCGGTGACAACGGCAACATGGCCCT32.0689655172413794No Hit
ATTGGGGGCCATGGGCTGGCTGATTTCAGTCAAAGGCCACACATTTGATC32.0689655172413794No Hit
GACGTCCCCCATAGATCTTGGTCATGGAGCCAACCCCAGCGCCACCCCGG32.0689655172413794No Hit
GTATCAACGCAGAGTACATGGGACATTGTGCAAACCTTCTCTCCTGTCAA21.3793103448275863No Hit
CATCTTCCGCCGGCGGATCTTCAGCACGTTTTTGCACTGAATTTGAGGCG21.3793103448275863No Hit
GTACATGGGATCTGTGCCCTGAGCATGGCTCTGCCCCCAGGTCGTCTTGC21.3793103448275863No Hit
TTGTGGAAGGTGTAGGGAGTGAAGTCTCAGATAAGAGGACCTGTGTGAGC21.3793103448275863No Hit
GAGTACATGGGTCCAGACCTCCTCTTGGCTTCGAGATGGCTTCGCCACAC21.3793103448275863No Hit
GACACAAGGTTGGGCTGTCTCTCCACCTCCTCACATTAGGCTAACAGGGA21.3793103448275863No Hit
GCAGGAGTGAGATGGAGGTGGACGCACCGGGTGTTGATGGTCGAGATGGT21.3793103448275863No Hit
TCTGTATCGCGGTAGGAGTTGGAATGAGGTGCAGGGTGGGCTTCAGGAAT21.3793103448275863No Hit
ACATGGGATCTGCTGGAGGTCTGTCGGGCAGATGTCAGGGCAGTAGGTGA21.3793103448275863No Hit
GGCCAGGGTCACCAAGGTCCTGGGCAGGACCGGTTCTCAGGGACAGTGCA21.3793103448275863No Hit
GAGTACATGGGGAAGCTGCAGGCTCCCTGCCCATAAAACAGGGTGTGAAA21.3793103448275863No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC10.6896551724137931No Hit
GCCCAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAATGATTGG10.6896551724137931No Hit
ACAGAGAAGGCGGCGCTTCCCAAAGGCACCGATGGAGTGCAGGGTAGCCG10.6896551724137931No Hit
CTTCTATGATGGAATCAAACGGCTCTATAATGAAGATAATGTCTCAGAAA10.6896551724137931No Hit
CCTCCGGGCTTCTCGCTCTGACTCCAAAAGGGTGAGCACGTCGCCCTCGC10.6896551724137931No Hit
AACGCAGAGTACATGGGATGGCTGTTCTGTGGGCCCTGGTGTCCGTGAGC10.6896551724137931No Hit
ATTTCAGCATCCTGCAGCTTCGAGCCAATGGTGAGCTCCTTCTGGATCAG10.6896551724137931No Hit
CACGAAGAGACCCGAACCAAGCAGGCCGCCCTGGATGGTGAGCCACTCGG10.6896551724137931No Hit
GTTGTTGTTGTTGTGCAAAGAATAAAAGAAATCATGAACACCGCTTATTC10.6896551724137931No Hit
TGGGAGACCTGGTGCTGACAGTGCTCATTGCCCTGGCCGTGTACTTCCTG10.6896551724137931No Hit
CTCCGTGCCTGACCATGTCGTCTGGTCCCTGTTCAACACCCTCTTCATGA10.6896551724137931No Hit
CTGAATAGCTCTAGGGATCTCAGCAGGGGTGGGAGGAACCAGCTCAACCT10.6896551724137931No Hit
GCCTTTATTGGGGGCCATGGGCTGGCTGATTTCAGTCAAAGGCCACACAT10.6896551724137931No Hit
TATCAACGCAGAGTACATGGGAACGAGAGACTGCTTGCTGCGGCAGAGAC10.6896551724137931No Hit
TCCTGATCTATCGCTGGGCCTGGACGACCAACACTGGGATGATGATGAGC10.6896551724137931No Hit
TCTAGGATCTGACTCCACTTCACTGTGAGGGCTCTGGCTCCACGGTGACG10.6896551724137931No Hit
CTATACATCTGGCCCCATCACCATGGAAACAACTCCAAAGCCTGCCTGGG10.6896551724137931No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG10.6896551724137931No Hit
GTACATGGGAGTCTCGCCAGCTGCCGGTCTTTCGGGGGCTCCGTAACTTT10.6896551724137931No Hit
CTTGCACCTCGGCGATCCCCGACTCCCTTCTTTATGGCGTCGCTCCTGTG10.6896551724137931No Hit
GCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTT10.6896551724137931No Hit
GCCTATGATATCAGTGAGGATGAAGAAGACTAATGTTTCCTCCCTCCTTT10.6896551724137931No Hit
CCGAAGATGTGTAGATCAATGACATATCCCTGCAGGATTACATTGCAGTG10.6896551724137931No Hit
CATCTTGCTTCCTTATGGTCCATGATGCCAGCCGAGGTTGTCAGTGCAAT10.6896551724137931No Hit
ATATGAGCAAGCTCAATGATGAATTTTCCTTCCTTATATTTCTGACTGCT10.6896551724137931No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG10.6896551724137931No Hit
GGCTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGC10.6896551724137931No Hit
GACTTATTTCTTCTTGGACACACCCACGGTGCGGCCACGGCGGCCAGTGG10.6896551724137931No Hit
AGTTCAGATAGTGAAAGGCCTGAAATATATGCTGGAGGTGGAAATTGGCA10.6896551724137931No Hit
CCATGGGCTGACTGATTTCAGTCAAAGGCCACACATTTGATCTTGAAGTC10.6896551724137931No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GCACAGGAGGACCCCAGCCCCATGCAGATGGTAGCCCAGGGGCGGGGGTG10.6896551724137931No Hit
CCTCGAAAAGGGAAGGAAAAGAAGGAAGAACAGGTCATCAGCCTCGGACC10.6896551724137931No Hit
GACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACAC10.6896551724137931No Hit
GTACATGGGACAGCCGGCAGCTTAGACAGGCGCCTGGGCCTCCAGAGGAG10.6896551724137931No Hit
ATCAACGCAGAGTACATGGGAGGGTGTGAAAGGCATCTCAGCGGCTGCCC10.6896551724137931No Hit
GCTCCGGACGAGGTACTGGGGGCTGGGGCGCAGGTCGAGAGCCCAGGGGG10.6896551724137931No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG10.6896551724137931No Hit
ATCCTTCAGTCTTGTTTCAATTAGATCATACTTTTGCCAGCGAGAGAGGG10.6896551724137931No Hit
CCTTCACATACTCCCAGCCCTCCTTGGAGTACTCGCGGGCCTTGGAGGGG10.6896551724137931No Hit
GTCTTCATCATTGTCCTCATCCCTGCTCTTGTCTTGCTCTTCCGAGTCTG10.6896551724137931No Hit
TCCTGGGAGAGGGACTGCGGGGCCTTTGGCAGAGCTCTGGCTGATGGAAC10.6896551724137931No Hit
GTCCAGGCCCAGGCCCAGAGCGATTGCAGTTGCTCTACGGTGAGCCCGGG10.6896551724137931No Hit
GAGTACGGTTTTTATTTTTTTTTTTTTTTTTTAAGGTAAAACCAACGCAC10.6896551724137931No Hit
GGTTGCCCAGGAGCATGGCTGCAAGGAGCAGGAGGGCCCCCAGGGTGACC10.6896551724137931No Hit
GTATCAACGCAGAGTACATGGGCCTCCACGCCGGCTCTTCCCCCCCCACA10.6896551724137931No Hit
GGTATCAACGCAGAGTACATGGGGACCTGTGACAAAGGAGTGTTCAGATT10.6896551724137931No Hit
GGAGGAAGTCACCTGGCTCCAGTGAACACGTGTGGAACAGAAATCAGGGC10.6896551724137931No Hit
TCTCTCAACTCCTCCTCGCATGGGTAGCGAGGAGGAGTTGAGAGAACGGA10.6896551724137931No Hit
GTATCAACGCAGAGTACATGGGAAAAACGCTAGAGCGGCGAGTTGTTACC10.6896551724137931No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.6896551724137931Clontech Universal Primer Mix Long (96% over 25bp)
ACAGATGGATGTAACTGAATCTACGAACGAGTGTACTTGCCCCAAATGTG10.6896551724137931No Hit
CCCTGTCTCTATCTCTCTGTCTCTCTCTCTCTCTCCGTCTCGATCTCTCT10.6896551724137931No Hit
GTATTGGGGAGCGGTCTGGTCTCTCAGGGATGGCACTCTGTGGGTCTGTA10.6896551724137931No Hit
TTTTTTGGTAGTCGGATTTGTTATGAATTTCTCTCTCCCCTCACCCCTCT10.6896551724137931No Hit
CCCCGAAATGCAGCGGCCCCACCGTCTCCTGAGGCCGGTGAGGCCAGGGG10.6896551724137931No Hit
ACTTGAGAGGGATGAAGCGGGAGGAGTGGGTGGCCCCGATGCCGGGCCGG10.6896551724137931No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCTGGGAACCA10.6896551724137931Clontech Universal Primer Mix Long (95% over 24bp)
CTCTGGGTGTGAGGCCTCCATATGGAGTCAGCAGAGGATCTGGGAGGGAT10.6896551724137931No Hit
ATCCTTCAGTCTTGTTTCAATTAGATCAAACTTTTGCCAGCGAGAGAGGG10.6896551724137931No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA10.6896551724137931No Hit
ATCAACGCAGAGTACATGGGTGAAGCGGAGGCGAAGGGGAGTTTAAGCCC10.6896551724137931No Hit
CTCTAAAGAAGTATCATTCTGGCCCAGCCTCCTCACTCATAGAAGTGCTC10.6896551724137931No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT10.6896551724137931No Hit
CCTGAAACACTTCTGCTGCTTTGACTGTGATAGCATTCTAGCTGGGGAGA10.6896551724137931No Hit
CCAATACCATGAAGCTCTGCGTGACTGTCCTGTCTCTCCTCATGCTAGTA10.6896551724137931No Hit
TCTGGGCCCTGATTTTGGGCATCTTCATGACCATTCTGCTCATCATCATC10.6896551724137931No Hit
GTTGACCAAAACACAGGAGCTGGGCCGTGACTACAGGACCTGTCTGACGA10.6896551724137931No Hit
TCCCGATTCACCCTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCC10.6896551724137931No Hit
CGCTCTGCTTTTTCATATTCTTCTGGCGGGCGAGCTCACGCTGGTTACCG10.6896551724137931No Hit
CCATTTTATAGATAGGTGACAGCTTCAGAGGCCACATTACCCAGTCAGTA10.6896551724137931No Hit
ACGCAGAGTACATGGGGGAAATCGCCAGCTTCGATAAGGCCAAGCTGAAG10.6896551724137931No Hit
ACGCAGAGTACATGGGAAGTCCGGACGCAGTAGCTCCCTGAAGCGGAGGC10.6896551724137931No Hit
GACCGTGATCCACATCCGCAGCGAGACCTCCGTGCCTGACCATGTCGTCT10.6896551724137931No Hit
ACCCTGGATGCCACCCCCCAGCGGGTACCAGAGGAAAGCTGGCAGCAGGC10.6896551724137931No Hit
TCCTCATCCCTGCTCTTGTCTTGCTCTTCCGAGTCTGTTTTTTTGTCTTT10.6896551724137931No Hit
GCTCCCTGAAGCGGAGGCGAAGGGGAGTTTAAGCCCCAGCGGCGGCAATG10.6896551724137931No Hit
ACTTTGGATAGCTATGGCTCGATACTTCTGGGTGCCCTCCTCCTAAGACA10.6896551724137931No Hit
ATGTTCTTGCTGTCGTTCTGTTGCAGCTCTTAGTCTGAGGTCCTCTGACC10.6896551724137931No Hit
AAATGACCCTTGGCTCTTATTGTTCTTGCTGGTGTGGTATGTTCACGGCT10.6896551724137931No Hit
TTATAGGGGTAGGGCCAGCTGGTGTTAATGGCATGCAGGCATATGTGATG10.6896551724137931No Hit
TCAACGCAGAGTACATGGGAGGCCATCATGGCGTCTCCCAGTGGGAAGGG10.6896551724137931No Hit
TCAACGCAGAGTACATGGGACATTGTGCAAACCTTCTCTCCTGTCAACAG10.6896551724137931No Hit
GTCTAGCTCTATCGCCAGGCTGGATTGGAGTGCAGTGGCACAATCTCAGC10.6896551724137931No Hit
AAGTATGCCAAGTACCTGCCTCACAGTGCAGGGCGGTATGCCGCCAAACG10.6896551724137931No Hit
CGTTGGGGGAGGAGGCGCCTGCTGCCAGCTTTCCTCTGGTACCCGCTGGG10.6896551724137931No Hit
GATTTCAGTCAAAGGCCACACATTTGATCTTGAAGTCACCGTCAGCTGCC10.6896551724137931No Hit
CTGCAGGAGAGACCCACAAAGAAGCCTCCTCCCTGTGGCCTGGCTCCCAT10.6896551724137931No Hit
GCCTAAGACAGAAATTCGGGAAAAACTAGCCAAAATGTACAAGACCACAC10.6896551724137931No Hit
GATGTCTATAGGAATATGCGTGTCAATCGCGCAGCGGGTCCCCTCCTCCT10.6896551724137931No Hit
CCCAAGTTCCCTCCCACATGGACGGGATGGAGGCTGGAGCCCAAGGTTTC10.6896551724137931No Hit
GGGCAGGACCGGTTCTCAGGGACAGTGCACGCAGGTGCGCGTGGAATTCA10.6896551724137931No Hit
GGTCGAAGGTGATGCACACCTCTGCAACACTTCCAGGCTGGAAGGGAAAG10.6896551724137931No Hit
GACTGGCAAGGTTTAAAAGTTTGAAGACTCTCACAGGTAAGTGCAGCTCA10.6896551724137931No Hit
CCATGGGCTGACTGATTTCAGTCAAAGGCCACACATGTGATCTTGAAGTC10.6896551724137931No Hit
GGTTGCTGACGTTCCCGGGATCTGAGGACAGGAGCCTGTGCTTCTTCCAC10.6896551724137931No Hit
CAAGCCACATGGGTGAGAGACGTGGTCAGGAGCATGGACAGGAAATCCAA10.6896551724137931No Hit
GGTATCAACGCAGAGTACATGGGAAGGACCCAGGAGTCTGGGTGCACAGC10.6896551724137931No Hit
GCCCCGCACTGCGGTGGTAAGGCAGAGTCTCAGTTCTCCTCGGGCTTGTC10.6896551724137931No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTAACTCTCCTTTGA10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
CACAGCTACAGCACTCTATGTACAAGCACATTGACGCTCCTGACTATCCT10.6896551724137931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)