FastQCFastQC Report
Tue 7 May 2024
EGAF00003666925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003666925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23924
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1550.6478849690687176No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1230.5141280722287243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.2048152482862398No Hit
TCTTTACATAGGTCTTCAAGAAGCGCGATCATAGAAATTATCCACCACAA430.179735830128741No Hit
CTATTAGTTTATGTAGATGGCCTGGTAGATAACGAGATTATCGATACCCA360.15047650894499248No Hit
CCTCTTTACGTATATGATGTGGCTTTAATACGTGAGCGTGCTAAAAGCTT360.15047650894499248No Hit
GTCCCGGACCGCGTCGCGCAATCGGCGGCTGGCGGCTCCTGCTGCCGCTT340.1421167028924929No Hit
CTTCTACTCCGGGCGTGATCCGAAGAAGAACATCGATGGAGTGACCCGTT320.1337568968399933No Hit
GTTCTAATGCTGCGAAAATCCTGGTTTTATTTACGCAGAAAAAAGAAGGC320.1337568968399933No Hit
GTCGTATACCGACTGCAGCCGCTTCTTCATCGCATCGCCGCGCAGCCGCT310.12957699381374352No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG300.12539709078749373No Hit
CTCTTATTGTTTCCATGAAAGTGGATGCGTTCTGCCGGAAAGCCTGCTGC300.12539709078749373No Hit
ATGTACGGCTGTTGCAGCAGGCTTTCCGGCAGAACGCATCCACTTTCATG300.12539709078749373No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA290.12121718776124395No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA290.12121718776124395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12121718776124395No Hit
AACTAATAGAGCCGCCAATCCACCTGTACCACCGATTTTCATCCTGCGGT280.11703728473499415No Hit
GTATTAGTACGGAAGCTCTCGGAAAAACCATCCCGGGGGCCACGGATCAC280.11703728473499415No Hit
GAGTAGAAGCGCATACGCATGACTACATTACAACGGGCCAGGAAGATTCA270.11285738170874436No Hit
GTACGGCATCGATGCTCGCGGGATCGACCGGCAGCATGTCCCAGTTGCGC270.11285738170874436No Hit
GCCCTACGCGGGGCCGGTCGGCCCGATCGGCAGCAAGGTGCTGGTGGCCT270.11285738170874436No Hit
GTATACGACGGTCGGCAACGCGGCGAGTGGCACGGGCAATGTGGCTGCTG260.10867747868249457No Hit
CGTATATGATGTGGCTTTAATACGTGAGCGTGCTAAAAGCTTTAAGCAGG260.10867747868249457No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT260.10867747868249457No Hit
GTTCTTACACGGCACAAGCAGACAAAATCAACATGGTCATTTAGAAATCG260.10867747868249457No Hit
GTATACGACCGCTTTCCGCGTCTGGCCGAACGCCGCTCGCAGCGCGCCGA250.10449757565624478No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA250.10449757565624478No Hit
TATATGATGTGGCTTTAATACGTGAGCGTGCTAAAAGCTTTAAGCAGGCG250.10449757565624478No Hit
GGCCATAGCAGCCCATCACCAGCAGGTCGGCGCTCAGGTCGGCAGCCAGC240.10031767262999498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)