FastQCFastQC Report
Tue 10 May 2022
EGAF00003667001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003667001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22711
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAATCTCCTGCCCCTGCTCCGGGTACCCCAGGGCTGCCGCCCATCAG890.39188058649993396No Hit
ATCATAGGTTGCACCATGCGTCTCCGGGCATTAATGAACCAGTTGTTGAC730.3214301439830919No Hit
GTACATGGGAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC610.2685923120954603No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG560.2465765488089472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.24217339615164457No Hit
GTCCACGAGCAGTCCGTTGCCGCCGTTCATCAGCTTCGCGATGCGAACGA480.21135132755052619No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG450.1981418695786183No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC450.1981418695786183No Hit
GAATAATATATGGATTCGAGACCATGAGGATAGCGGGTCTGTACATTTGG410.18052925894940777No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG410.18052925894940777No Hit
CCCCAGGGCTGCCGCCCATCAGGGTAAAGTCCAAGTGGCCAACATGATGC400.17612610629210515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.17172295363480253No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG380.16731980097749988No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA360.15851349566289463No Hit
GGGTATTGTTGATCTTGAGAAGATTTTGTAGAGTGGCAGCAAATCCTGGT360.15851349566289463No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT360.15851349566289463No Hit
ATATTATTCTGGTCTGGGAGGCTGAGGCAGGAGGCTGGGTAGTCCTCGAA350.154110343005592No Hit
GTCCTACTTGCCATAGGGAAGGAGACATTCCATTGAGGTCTTGGGTATTC340.1497071903482894No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA320.14090088503368411No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT320.14090088503368411No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC310.1364977323763815No Hit
GTACATGGGGAGGGGGTTGGCGAAGCACACGAAGTGCGCGAAGCCTGGGG310.1364977323763815No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA310.1364977323763815No Hit
GTATTGTTGATCTTGAGAAGATTTTGTAGAGTGGCAGCAAATCCTGGTTG310.1364977323763815No Hit
TATCAGCACTGTCAGTTGGGGTCTGTCCCCAGGACTCCATCCATGTTGCC300.13209457971907887No Hit
GGACAACCAGTGGACGCAGCTGCTCGTCGGCGGCGCCGCGGGCGCGGCCT300.13209457971907887No Hit
GTACATGGGCACCAGGGGCTGGCTCTCGGCGTCCTGGAGCTTCGAGAGCA290.12769142706177622No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG290.12769142706177622No Hit
GCCTTGGCGGGGTAGCCCGCGACCTTCAGGTCCTCGGCCACGCCCTTCTT290.12769142706177622No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC280.1232882744044736No Hit
CCAGTGGACGCAGCTGCTCGTCGGCGGCGCCGCGGGCGCGGCCTCTGCCG280.1232882744044736No Hit
ATGTTGAACCCCGTGTCGGCGAACCCCGTGAAGGACAACCAGTGGACGCA280.1232882744044736No Hit
CACACACACAGTGGCCAAAAAGAAAGCCTCACTGTGTAATCTCCTGCCCC280.1232882744044736No Hit
AGCCGGGAGCCTGCGCTGCTCCCTGCGGCCATGCCCGTGCGGGCGAGGCC280.1232882744044736No Hit
TTCCAGGGCAACCCGGTGGCACGCGAGATCGACTTCAAGAGTTCCTGCGA270.11888512174717097No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA260.11448196908986834No Hit
CACACACAGTGGCCAAAAAGAAAGCCTCACTGTGTAATCTCCTGCCCCTG260.11448196908986834No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA250.11007881643256572No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC250.11007881643256572No Hit
GTCTATGGGGACAGTGAGTACAGCCAGCTCCTCAGAGGGCACGTGGCCTC250.11007881643256572No Hit
CTATGGGGACAGTGAGTACAGCCAGCTCCTCAGAGGGCACGTGGCCTCCG250.11007881643256572No Hit
GTACATGGGCCCCGTGATGGGGCCGGAGCTGGGCTGGGGACACCCCGCGG250.11007881643256572No Hit
GCGCTACCTGGTGCAGAACTCGGTGAGCTATGAGAACGTGGCCGGCGCGC250.11007881643256572No Hit
ATGTTGGTGGCCACCTTGGGGAAGATCCCCCTCTTCTTGTTTCGCCGTGG250.11007881643256572No Hit
GAACTGGAAGATTGTCATTACTGCCGCAGCATGGAGACCCAGGATGGGAG250.11007881643256572No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC240.10567566377526309No Hit
GTGGTGGACCGCGCCAGCGGCGAGGTCATCCGCCAGATTCCCAACGAGGA240.10567566377526309No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG240.10567566377526309No Hit
ACATGATGCCTCGGGCCCTCCAAACCCAACCTTGCCAGCTGGCCCAGCTT240.10567566377526309No Hit
GTACATTTGGGGACCCCAGGTCCATCCAGCGGGGGCCTGGCCTCCCAGAG240.10567566377526309No Hit
GTCCAGGTTGGAGTGCAGGGGCGCAATCTCGGCTCACTGCAACCTCTGCC240.10567566377526309No Hit
GTCCCCAGGACTCCATCCATGTTGCCACCATCTCTCCCTGGGCATCTGCT240.10567566377526309No Hit
GTACATGGGAGGGGCGTGTGGCTCACGTGTGTGACTCAGGTGTCTCTGGC230.10127251111796046No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG230.10127251111796046No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA230.10127251111796046No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT230.10127251111796046No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT230.10127251111796046No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT230.10127251111796046No Hit
GTCCACCACCTTGACGATGACCATGTCGGTCTCCTTGTCGACCTCGAACT230.10127251111796046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.10127251111796046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)